Reduced CD38 expression on CD34+ cells as a diagnostic test in myelodysplastic syndromes.

Department of Haematology, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, Oxford, Oxford, UK.
Haematologica (Impact Factor: 5.87). 09/2009; 94(8):1160-3. DOI: 10.3324/haematol.2008.004085
Source: PubMed

ABSTRACT Diagnosis of myelodysplastic syndrome can be difficult especially in cases with a low blast count and a normal karyotype. Flow cytometry has been used to distinguish myelodysplastic syndrome from non-clonal cytopenias. No one single simple flow cytometric parameter has been proposed to be diagnostic of myelodysplastic syndrome. We have studied samples from 100 myelodysplastic syndrome patients and as control samples; 70 non-clonal cytopenias, 5 subjects with normal hematology, 31 patients with acute myeloid leukemia and 11 with chronic myelomonocytic leukemia or myeloproliferative disorder. We show that reduced relative mean fluorescence of CD38 below a threshold value on CD34(+) cells diagnosed low-grade myelodysplastic syndrome with 95% sensitivity (95% confidence interval, 87-99%) and 92% specificity (95% confidence interval, 82-97%). This simple flow cytometric test may be of value in the routine clinical diagnosis of myelodysplastic syndrome, especially in cases with a low blast count and normal karyotype.

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    PLoS ONE 02/2014; 9(2):e88706. DOI:10.1371/journal.pone.0088706 · 3.53 Impact Factor
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    ABSTRACT: The increased LSC in MDS has correlation with the progression to AML, which the mechanism of immune evasion is unclear. Our study showed the expression of CD47 on LSC of the patients in high-risk MDS based on IPSS/WPSS score was higher than that of in low-risk MDS and controls. The level of CD47 on erythroblast of MDS patients had a significant positive correlation with their peripheral RBC count. It suggested that the proportion of CD34(+)CD38(-)CD47(+) cells increased in high-risk MDS which might protect LSC from avoiding phagocytosis, and low-expression of CD47 on erythroblast in MDS might be correlated to anemia.
    Leukemia research 05/2013; 37(8). DOI:10.1016/j.leukres.2013.04.008 · 2.69 Impact Factor
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    ABSTRACT: BACKGROUND: Abnormal proliferation and differentiation of hematopoietic stem/progenitor cells, which reflect the malignant nature of clonal cells in myelodysplastic syndromes (MDS), can be detected by flow cytometry (FCM) and potentially applied to assist diagnosis and evaluate prognosis in MDS. METHODS: In this study, a series of immunophenotypes such as CD34, CD19, CD38, CD117, and CD7, which are related to proliferation and differentiation of HSCs, were determined by FCM in the patients with nonclonal cytopenias diseases and MDS. Based on the expression pattern of these immunophenotypes, a FCM progress scoring (FPS) system was constructed and evaluated. RESULTS: The FPS system showed good sensitivity and specificity (63.6% and 100.0%) in distinguishing MDS from nonclonal cytopenias diseases. Validation analysis of FPS system indicated comparable sensitivity and specificity (73.7% and 97.1%) and high agreement rate (82.6%) of FCM diagnosis with morphological diagnosis. The high-grade MDS had higher FPS score compared to low-grade MDS (P < 0.001). Noticeably, hypocellular MDS had lower FPS score (P < 0.001), most of which could not be diagnosed by FPS system. Besides, FPS score showed obvious positive correlation with WHO classification, IPSS score, percentage of marrow blasts, and cytogenetic prognosis scoring. Elevated FPS score predicted higher disease progression and shorter survival in MDS. CONCLUSION: The FPS system based on immunophenotyping in CD34+ blasts is a useful and simple tool for diagnosis and prognosis evaluation in MDS. © 2013 International Clinical Cytometry Society.
    Cytometry Part B Clinical Cytometry 07/2013; 84B(4). DOI:10.1002/cyto.b.21089 · 2.28 Impact Factor

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