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Human mesenchymal stem cell differentiation on self-assembled monolayers presenting different surface chemistries

Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA 30332, USA.
Acta biomaterialia (Impact Factor: 5.68). 08/2009; 6(1):12-20. DOI: 10.1016/j.actbio.2009.07.023
Source: PubMed

ABSTRACT Human mesenchymal stem cells (hMSCs) have tremendous potential as a cell source for regenerative medicine due to their capacity for differentiation into a wide range of connective tissue cell types. Although significant progress has been made in the identification of defined growth factor conditions to induce lineage commitment, the effect of underlying biomaterial properties on functional differentiation is far less understood. Here we conduct a systematic assessment of the role for surface chemistry on cell growth, morphology, gene expression and function during hMSC commitment along osteogenic, chondrogenic and adipogenic lineages. Using self-assembled monolayers of omega-functionalized alkanethiols on gold as model substrates, we demonstrate that biomaterial surface chemistry differentially modulates hMSC differentiation in a lineage-dependent manner. These results highlight the importance of initial biomaterial surface chemistry on long-term functional differentiation of adult stem cells, and suggest that surface properties are a critical parameter that must be considered in the design of biomaterials for stem cell-based regenerative medicine strategies.

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    • "Table 2 summarizes the main findings of the described studies. Phillips and co-authors analyzed [14] SAMs functionalized with four different functional groups, namely methyl (–CH 3 ,), hydroxyl (–OH), carboxyl (–COOH) and amino (–NH 2 ), and were able to demonstrate that the surface chemistry has an effect on the pattern of Fn adsorption, which in turn modulates the osteogenic differentiation of human MSCs (hMSCs). Differences in Fn conformation promoted different integrin–ligand interactions and the consequent activation of different intracellular signaling pathways [85]. "
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    Acta biomaterialia 08/2013; 9(11). DOI:10.1016/j.actbio.2013.08.004 · 5.68 Impact Factor
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    • "The cellular response to biomaterial surfaces, such as attachment and proliferation, is known to be mediated by the material's surface chemistry. Indeed, surface chemistry is also able to instruct cell function and direct cell differentiation [1] [2] [3] [4] [5] [6] [7] [8] [9] [10]. However, it has proven difficult to predict cell fate outcomes based on the molecular structure of the polymer substrate [11] [12]. "
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