Stability of blood analytes after storage in BD SST (TM) tubes for 12 mo
ABSTRACT Objectives: We studied the stability of 33 analytes related to clinical chemistry, bone, and vitamin metabolism, after storage in serum separator tubes (SST (TM)). Design and methods: Blood was collected from 6 subjects using SST tubes. Some serum remained in the tube in contact with the barrier gel and was stored at -80 degrees C for 12 mo. Results: Clinically significant changes Occurred only in 1,25-dihydroxyvitamin D and retinol-binding protein. Conclusions: Freezing SST tubes before sample analysis is a viable option for some analytes.
- Clinical Chemistry and Laboratory Medicine 09/2014; 53(3). DOI:10.1515/cclm-2014-0543 · 2.96 Impact Factor
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ABSTRACT: Abstract Context: In epidemiological research, it is very important to test the stability of biomarkers as function of both storage time and temperature. Objective: In this study, the stability of biomarkers of the iron status was tested up to 1 year of storage. Materials and methods: The biomarkers include total iron, unsaturated iron binding capacity, ferritin, transferrin, soluble transferrin receptor, ceruloplasmin and haptoglobin. Results: The concentrations of all biomarkers tested remain constant upon storage at -20, -70 and -196 °C. Conclusion: All biomarkers of the iron status were stable at the temperatures tested for 1 year.Biomarkers 04/2013; DOI:10.3109/1354750X.2013.781223 · 2.52 Impact Factor
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ABSTRACT: To assess a wide spectrum of biochemical markers (biomarkers) in a large cohort of individuals with (very) early symptomatic knee and/or hip osteoarthritis (OA). Secondly, to investigate associations between biomarkers and between biomarkers and demographics to demonstrate validity of the obtained dataset and further investigate the involvement and/or role of these biomarkers in OA. Fourteen biomarkers (uCTX-II, uCTX-I, uNTX-I, sCOMP, sPIIANP, sCS846, sC1,2C, sOC, sPINP, sHA, sPIIINP, pLeptin, pAdiponectin, pResistin) were assessed by ELISA or RIA in CHECK (Cohort Hip and Cohort Knee), a 10-year prospective cohort of 1,002 individuals with early symptomatic knee and/or hip OA. Quality controls revealed that gathered data were technically reliable. The majority of biomarkers showed relevant associations with demographic variables, which were expectedly different between genders and/or menopausal status for some. Principal component analysis enabled identification of five clusters, consecutively designated as 'bone-CTX-II', 'inflammation', 'synovium', 'C1,2C-adipokines', and 'cartilage synthesis' cluster. Notably, uCTX-II clustered with biomarkers of bone metabolism, while sCOMP clustered with biomarkers of synovial activity. The identified clusters extended knowledge on individual biomarkers from mostly smaller studies as did the observed associations between biomarker levels and demographics, from which validity of our data was deduced. uCTX-II may not only reflect articular cartilage but also bone metabolism and sCOMP may reflect synovial rather than cartilage metabolism. Major involvement of adipokines in joint metabolism was not identified.Osteoarthritis and Cartilage 04/2012; 20(7):745-54. DOI:10.1016/j.joca.2012.04.004 · 4.66 Impact Factor