Pygeum africanum: effect on oxidative stress in early diabetes-induced bladder.
ABSTRACT To evaluate the effect of Pygeum africanum on oxidative stress and functional changes of the bladder after diabetes induction.
Thirty-two adult Wistar male rats were treated daily for 8 weeks and grouped as follows: Control group (n = 6), Streptozotocin-induced diabetic group (n = 10), diabetes plus P. africanum group (n = 10), and control plus P. africanum group (n = 6). After diabetes induction for 4 weeks, the diabetes plus P. africanum and control plus P. africanum groups were fed with P. africanum (100 mg/kg, orally) in peanut oil for another 4 weeks. The catalase, superoxide dismutase activity, and malondialdehyde levels were measured as a marker of lipid peroxidation. The levels of inducible nitric oxide synthase were also evaluated. Urodynamic studies were performed to evaluate the functional changes of diabetic bladders after P. africanum treatment.
The catalase and superoxide dismutase activities significantly increased (P < 0.05) and maleic dialdehyde levels significantly decreased from diabetic plus P. africanum group compared with diabetic group (P < 0.05). Immunohistochemical studies showed a significantly decreased number of inducible nitric oxide synthase-positive cells in diabetic plus P. africanum group compared with diabetic group (P < 0.05). In diabetic plus P. africanum group, maximal bladder volume significantly decreased, while bladder pressure and maximal bladder pressure significantly increased compared with diabetic group (P < 0.05).
Early treatment with P. africanum could effectively suppress the oxidative stress status in diabetic bladder and may slow down the process of diabetic cystopathy.
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ABSTRACT: Selenite cataract, as an experimental animal model of nuclear cataract to mimic human senile cataract, is produced only when overdose selenite is injected to neonatal rats before eyelid opening. To clarify the cause of age differences on selenite cataract formation in rats, mRNA expression of GPx1, MsrA and MsrB1, as well as GPx activity in Wistar rat lens at different ages were assayed, level of lipid peroxidation, extent of lens damage induced by sodium selenite and barricade function of blood-retinal barrier (BRB) were investigated. The results showed that mRNA expressions and activity of antioxidant enzymes in neonatal rat lens before eyelid opening were the highest and then decreased with age, and revealed by transmission electron microscopy (TEM) using lanthanum hydroxide as tracer that higher selenite content entering eyes injured lens and resulted in cataract formation for immature BRB before eyelid opening, moreover, a little selenite content entering eyes was not enough to induce cataract formation after eyelid opening because of mature BRB.Toxicology Letters 11/2012; 216(2-3). DOI:10.1016/j.toxlet.2012.11.016 · 3.36 Impact Factor
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ABSTRACT: BACKGROUND: Several lines of evidence suggest that the generation of reactive oxygen species (ROS) is of major importance in the pathogenesis of scleroderma, and thus antioxidant therapy may be useful for patients with an impaired oxidative defence mechanism. AIM: To examine the effect of N-acetylcysteine (NAC) on skin fibrosis and oxidative stress in a bleomycin (BLM)-induced mouse model of scleroderma. METHODS: We used this mouse model to evaluate the effect of NAC on skin fibrosis and oxidative stress. Skin fibrosis was evaluated by histopathological examination and hydroxyproline content. To measure lipid peroxidation, we used a thiobarbituric acid-reactive species, malondialdehyde (MDA). Oxidative protein damage (carbonyl content) and the activities of catalase (CAT) and superoxide dismutase (SOD) were determined to evaluate oxidative stress in the skin tissue. RESULTS: Treatment with NAC attenuated the skin fibrosis induced by BLM, significantly reducing the MDA and protein carbonyl content in these mice. SOD activity in BLM-only mice and BLM plus NAC-treated mice was increased compared with control mice. However, there was no significant difference in skin SOD activity of mice treated with both BLM and NAC compared with those treated with BLM only. In addition, CAT activity was not altered in the BLM plus NAC mice. CONCLUSIONS: NAC treatment attenuates skin fibrosis in a BLM-induced mouse model of scleroderma, and this is associated with diminished oxidative stress. The results suggest that NAC may be a potential therapeutic agent for patients with scleroderma.Clinical and Experimental Dermatology 03/2013; 38(4). DOI:10.1111/ced.12033 · 1.23 Impact Factor
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ABSTRACT: Clinical research has revealed that stressed men are susceptible to develop benign prostatic hyperplasia (BPH). In this study, restraint-stress mice model was employed to mimic the physiological conditions of the population that was susceptible to develop BPH. Male mice were subjected to restraint stress after being subcutaneously injected with testosterone propionate (TP) for 14 cl. Results demonstrated that TP-induced BPH was significantly aggravated by restraint stress, as manifested by increases of prostate index, serum testosterone level, and prostate 5 alpha-reductase (5AR) and serum acid phosphatase (ACP) activities. These findings were further supported by results of prostate pathological examination. However, we found that anthocyanins extract (AE) from bilberry (Vaccinium myrtillus L.) had additive effect with pollen of Brassica napus L. (PBN), a widely used folk remedy for BPH in traditional Chinese medicine, on stress-provoked BPH in mice. The mechanism was associated with the protective effects of AS against stress-induced oxidative damage, as indicated by decreased lipid peroxidation level, increased oxygen radical absorbance capacity (ORAC) and glutathione (GSH) content, along with elevated superoxide dismutase (SOD) and glutathione peroxidase (GPx) activities. Our results proved that-stress-induced oxidative damage promoted the development and aggravation of BPH, while antioxidative defense contributed to the amelioration of BPH.Journal of Functional Foods 07/2013; 5(3-3):1357-1365. DOI:10.1016/j.jff.2013.05.003 · 4.48 Impact Factor