Global Discovery of Adaptive Mutations

Department of Molecular Biology & Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, New Jersey, USA.
Nature Methods (Impact Factor: 32.07). 09/2009; 6(8):581-3. DOI: 10.1038/nmeth.1352
Source: PubMed


Although modern DNA sequencing enables rapid identification of genetic variation, characterizing the phenotypic consequences of individual mutations remains a labor-intensive task. Here we describe array-based discovery of adaptive mutations (ADAM), a technology that searches an entire bacterial genome for mutations that contribute to selectable phenotypic variation between an evolved strain and its parent. We found that ADAM identified adaptive mutations in laboratory-evolved Escherichia coli strains with high sensitivity and specificity.

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Article: Global Discovery of Adaptive Mutations

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    • "Under this approach, a set of synthetic oligonucleotides representing the mutations found by WGS randomly undergoes recombination with genomic DNA, creating a library of allelic replacement strains. Second, array-based discovery of adaptive mutations (ADAM) uses genetic foot printing to selectively identify mutations that provide a competitive advantage to the cell (Goodarzi et al, 2009). When reconstructing phenotypes through allelic replacement, ADAM allows the researcher to avoid the wasted effort of reconstructing non-beneficial mutations. "
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