Article

[Ln(DPA)(3)](3-) is a convenient paramagnetic shift reagent for protein NMR studies.

Research School of Chemistry, Australian National University, Canberra, Australia.
Journal of the American Chemical Society (impact factor: 9.91). 08/2009; 131(30):10352-3. DOI:10.1021/ja9034957 pp.10352-3
Source: PubMed

ABSTRACT Paramagnetic lanthanide ions present outstanding tools for structural biology by NMR spectroscopy. Here we show that the 3:1 complexes between dipicolinic acid and lanthanides are paramagnetic reagents which can site-specifically bind to a wide range of proteins without formation of a covalent bond. The observed pseudocontact shifts can be interpreted by a single magnetic susceptibility anisotropy tensor, enabling its use for structure refinements. The resonance assignment of the paramagnetic spectrum is greatly facilitated by the rapid exchange between bound and free protein, leading to gradual chemical shift changes as the protein is titrated with the paramagnetic dipicolinic acid complex. The association with the paramagnetic lanthanide leads to weak molecular alignment in a magnetic field so that the reagents can be used for the measurement of residual dipolar couplings without the need of protein modification or anisotropic alignment media. The protein samples can be recovered by simple dialysis.

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    Article: The Utility of 2, 2-Bipyrimidine in Lanthanide Chemistry: From Materials Synthesis to Structural and Physical Properties
    Gaël Zucchi
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    ABSTRACT: This paper reviews the recent investigations undertaken on the use of 2,2 -bipyrimidine (bpm) as a ligand for designing molecular complexes as well as polymeric lanthanide materials. A special emphasis is put on the ability of this polydentate neutral ligand to yield compounds of various dimensionalities, to act as a connector between these large ions, and influence their emissive and magnetic properties. This ligand can adopt a terminal or a bridging coordination mode with lanthanide ions, thus generating a wealth of frameworks of various topologies with the 4f elements. The main focus of this review is to show the originality brought by bpm in lanthanide structural chemistry and solid-state photophysics and magnetism.
    Hindawi Publishing Corporation International Journal of Inorganic Chemistry. 01/2011; 13.
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Keywords

anisotropic alignment media
 
gradual chemical shift changes
 
NMR spectroscopy
 
observed pseudocontact shifts
 
paramagnetic dipicolinic acid complex
 
paramagnetic lanthanide
 
Paramagnetic lanthanide ions present outstanding tools
 
paramagnetic spectrum
 
protein modification
 
rapid exchange
 
reagents
 
residual dipolar couplings
 
simple dialysis
 
single magnetic susceptibility anisotropy tensor
 
wide range