Article

Improvement of a real-time RT-PCR assay for the detection of enterovirus RNA

Department of Microbiology, ZNA Hospitals, site Middelheim, Lindendreef 1, 2020 Antwerp, Belgium.
Virology Journal (Impact Factor: 2.09). 02/2009; 6:95. DOI: 10.1186/1743-422X-6-95
Source: PubMed

ABSTRACT We describe an improvement of an earlier reported real-time RT-PCR assay for the detection of enterovirus RNA, based on the 5' exonuclease digestion of a dual-labeled fluorogenic probe by Taq DNA polymerase. A different extraction method, real-time RT-PCR instrument and primer set were evaluated. Our data show that the optimized assay yields a higher sensitivity and reproducibility and resulted in a significant reduced hands-on time per sample.

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