Article

Estudio comparativo de dos técnicas de cromatografía de líquidos para la determinación de anfetamina y derivados en sangre y orina: CLAR-fluorescencia vs. CL-EM

Revista de Toxicología 01/2005;
Source: DOAJ

ABSTRACT El consumo de psicoestimulantes, tales como la anfetamina y derivados (éxtasis), ha experimentado un aumento en España en los últimos años. Así, el número de incautaciones de ¿pastillas¿ ha aumentado un 62,35% entre 2001 (860.000) y 2002 (1.396.000), según datos del Observatorio Español sobre Drogas (OED), situándose la máxima prevalencia del consumo de éxtasis, entre los 20-24 años en varones (14,3%), y entre los 25-29 años (5,1%) en mujeres. Por este motivo, es muy útil disponer en un laboratorio de toxicología forense, de un método rápido, sencillo y específico para la determinación de estas sustancias. En este trabajo se describen dos técnicas analíticas para la determinación de los derivados anfetamínicos en sangre y orina, concretamente de MDMA, MDA, MDEA y MBDB mediante cromatografía de líquidos de alta resolución con detector de fluorescencia (CLAR-Fluorescencia), y de anfetamina, metanfetamina, PMA, MDA, MDMA, MDEA y MBDB mediante cromatografía de líquidos acoplada a espectrometría de masas (CL-EM). Ambos métodos, validados y aplicados al análisis de casos reales que llegaron a nuestro Servicio de Toxicología, son comparados indicando sus ventajas e inconvenientes (CLAR-Fluorescencia, técnica más sencilla y económica; CL-EM, técnica más sensible y específica)

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