Antimicrobial Activities of Lactic Acid Bacteria Strains Isolated from Burkina Faso Fermented Milk
ABSTRACT Eight strains of lactic acid bacteria producing bacteriocin were isolated from Burkina Faso fermented milk samples. These strains were identified to species: Lactobacillus fermentum, Pediococcus spp., Leuconostoc mesenteroides subsp. meseteroides, Lactococcus. Isolated bacteriocin exhibited antibacterial activity against Enterococcus faecalis 103907 CIP, Bacillus cereus 13569 LMG, Staphylococcus aureus ATCC 25293, Escherichia coli 105182 CIP using the agar drop diffusion test. The inhibition diameters obtained with bacteriocin are between 8 mm and 12 mm. Gram-positive indicator bacteria were most inhibited. The activities of the bacteriocin were lost after treatment with all the proteolytic enzymes (alpha-chymotrypsin, trypsin, pesin), whereas treatment with lipase, catalase, alpha-amylase did not affect the activity of the bacteriocin.
Full-textDOI: · Available from: Imael H N Bassolé, Apr 19, 2015
SourceAvailable from: Prof. Ashraf Mahdy Sharoba[Show abstract] [Hide abstract]
ABSTRACT: Lactobacillus strains are a major part of the probiotics, microflora of the intestine as well as of fermented dairy products, and are found in a variety of environments. The aim of this study was to find out the ability of bile salts and acid tolerance as well as antibacterial properties of the three lactobacillus strains namely Lactobacillus acidophilus ATCC 20552, Lactobacillus casei DSM 20011 and Lactobacillus plantarum ATCC 14917. The acid tolerance test was studied under pH 2.0 and 3.0 with 6.5 as a control. The cell count for the acid tolerance test was obtained at an interval of 0, 1, 2 and 3 hours, and was pour plated on Man Rogosa, and Sharpe (MRS) agar to be incubated at 37 °C for 24 hours. All cells were selected for bile tolerance test in MRS broth containing bile concentrations of 0 % as control and 0.1, 0.3, 0.5 and 0.7 % as test and antibiotic susceptibility. Results showed that the three strains of Lactobacilli have ability to tolerate acid and bile salts and resistance to 0.4 % of phenol and were susceptible for streptomycin (10 µg), ampicilline (10 µg), chloramphenicol (30 µg), erytromycin (15 µg), amoxicillin/clavulanic acid (30 µg) and rifampicin (5 μg). All strains have antimicrobial activity against seven indicator bacteria included Bacillus cereus DSM 351, Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 12600, Listeria monocytogenes, Salmonella typhimurium ATCC14028, Klebseilla pneumonia ATCC 1705 and Pseudomonas aeruginosa ATCC 43495.
African journal of microbiology research 03/2014; 8(10):1012-1016. DOI:10.5897/AJMR2014.6632 · 0.54 Impact Factor
Dataset: بحث احمد حسانين