Allanblackia floribunda is a tree employed in Nigeria and other countries to treat skin disease and other microbial diseases. The ethanol extract, n-hexane, chloroform, ethyl acetate and butanol fractions of the leaves, stem bark and root bark were evaluated for antimicrobial activities against Staphylococcus aureus NCIB 8588, Bacillus subtilis NCIB 3610, Escherichia coli NCIB 86, Proteus vulgaris NCIB 67, Pseudomonas aeruginosa NCIB 950, Klebsiella pneumoniae NCIB 418, Candida albicans and Aspergilus flavus , using agar diffusion method to validate the ethnobotanical uses of the plant. Among the extracts, the ethanol extract of the leaf gave the most significant antibacterial activity. However, no extract showed antifungal activity. Generally, the fractions obtained from the extracts elicited better activity, including antifungal activity against C. albicans . The highest inhibitory effect was exhibited by leaf extract against Ps. aeruginosa NCIB 950, while the ethyl acetate fraction of the stem bark gave the least inhibitory effect against B. subtilis NCIB 3610. The plant extract and fractions produced inhibition zone range between 5 and 35 mm.
"Its bark has long been used to cicatrize wound (Okunomo and Egho, 2010), and for the treatment of leprosy, dysentery, anaemia, spitting blood, debility, stiffness, tonsillitis and skin diseases (Dalziel, 1937; Hutchinson et al., 1963). The leaves are often crushed and the juice released to treat generalized skin diseases such as scabies, ringworm, rash and wound, while the stem or stem twigs are employed as chewing sticks for oral hygiene (Igoli et al., 2005; Ajibesin et al., 2008). When chewed with kolanut, its leaves serves as an antiemetic, while its leaf sap could be used for treating ear infections, fever, headache, malaria and cephalgy (Bouet, 1980). "
[Show abstract][Hide abstract] ABSTRACT: African pear, Dacryodes edulis, seed extract has been used for the treatment of ailments in traditional medicine. However, the effect of boiling on its antioxidant properties is still poorly understood. Therefore, the present study investigates the effect of boiling on the antioxidant properties of D. edulis seed extract using in vitro parameters such as free radical scavenging ability against 2, 2-diphenyl -1-picrylhydrazyl (DPPH) radical, iron (III) reducing and iron (II) chelating ability. Furthermore, the ability of both extracts (boiled and unboiled) to offer protective benefit against lipid peroxidation in cerebral and hepatic tissues of rat was assessed. Moreover, the effect of boiling on the phytochemical constituents (total phenolics, flavonoids and vitamin C) of the seed extracts was measured. Results indicate that boiling significantly (P < 0.05) potentiates the total phenolic [(Boiled 60.1 ± 0.88 mg/g (GAE); Unboiled 30.2 ± 0.68 mg/g (GAE)] and flavonoid [(Boiled: 50.02 ± 0.12 mg/g (QE); Unboiled: 35.8 ± 0.15 mg/g (QE)] content but mildly depleted the vitamin C content [Boiled: (36.9 ± 0.44 mg/g; Unboiled: 40.1 ± 0.21 mg/g]. Similarly, boiling markedly increased the antioxidant properties (free radical scavenging, iron (II) chelating, iron (III) reducing and inhibitory effect against pro-oxidant-induced lipid peroxidation) of the seed extract. From the foregoing, the wide usage of African pear as remedy for ailment in folk medicine may be due to its phytochemical constituents which are potentiated by boiling. Hence, information from this study would create public awareness especially to traditional medical practitioners who are involved in the act of boiling the fruit to get the extract used for medicinal purposes.
[Show abstract][Hide abstract] ABSTRACT: Allanblackia floribunda Oliver (Guttiferae) is an African medicinal plant used traditionally to treat a variety of ailments.
We investigated the antitumor, radical scavenging, antimycobacterial, antibacterial and antifungal activities of the root bark extract of A. floribunda and three isolated phenolics, namely 1,7-dihydroxyxanthone (1), morelloflavone (2) and 7'-O-glucoside of morelloflavone (3).
The 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) radical scavenging assay was used for antioxidant tests, while crown gall tumor assay was used for assay of antitumor activity. The p-iodonitrotetrazolium chloride (INT) colorimetry and Microplate Alamar Blue Assay (MABA) were used for antimicrobial investigations.
Moderate tumor reducing activity was observed with the extract, while better activities were recorded with compounds 2 and 3. The antimycobacterial and antitumor activities of the extract are being reported for the first time. The DPPH radical scavenging test showed that all the studied samples were able to scavenge more than 50% of the free radical, with compound 3 showing the best inhibitory activity (IC(50) of 49.08 µg/mL). Compounds 1 to 3 prevented the growth of Mycobacterium smegmatis and both extract and compound 2 were active on M. tuberculosis. The lowest MIC value for the extract (9.76 μg/mL) was recorded against Enterobacter aerogenes while the corresponding value for the compounds (4.88 µg/mL) was obtained with compound 2 on Trichophyton rubrum.
The overall results of the present work provide baseline information for the potential use of the root bark extract of A. floribunda as an antimicrobial, antitumor and antioxidant phytomedicine.
[Show abstract][Hide abstract] ABSTRACT: This study was carried out to investigate the claims on seed of Dacroydes edulis as a remedy for stomach ailments. Boiled seed extracts of D.edulis were subjected to phytochemical and antimicrobial analysis. Results of phytochemical screening showed presence of reducing sugar, free anthraquinone, saponins, tannins, steroid/ triterpenes, flavonoids and alkaloids. The anti-microbial screening result of extracts (hexane, chloroform, ethyl acetate and methanol)showed sensitivity against the following disease causing human pathogens: Staphylococcus aureus, Escherichia coli, Salmonella typhi, Shigella dysenterea, Pseudomonas aeruginosa, klebsiella pneumoniae, Candida albicans, Trichophytom rubrum and Microsperum sp. Chloroform extract exhibited highest inhibition against the pathogens that were sensitive to the extracts, the pathogen Klebsiella pneumoniae was most inhibited (32 mm).For Minimum inhibitory concentration (MIC), these extracts showed inhibition against all the pathogens. Chloroform, hexane and methanol extracts showed inhibitory effects at concentration of 1.25 mg/mL for most of the pathogens with MIC value of 0.625 mg/mL for Escherichia coli, Klebsiella pneumoniae and Shigella dysenteriae.The minimum bactericidal concentration/minimum fungicidal concentration (MBC/MFC) of extracts showed inhibition against all the pathogens at values of 5, 2.5 and 1.25 mg/mL. This justifies the trado-medicinal claims on Dacroydes edulis as remedy for stomach ailments.
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