Effect of ascorbic acid on reduced glutathione level in arsenic-loaded isolated liver tissues of rat

Bangladesh Journal of Pharmacology (Impact Factor: 0.51). 01/2006; DOI: 10.3329/bjp.v1i2.491
Source: DOAJ

ABSTRACT Isolated liver tissues of rat were loaded with trivalent arsenic and were exposed in presence and absence of ascorbic acid. The amount of reduced glutathione (GSH) in normal liver tissue was 52.0  0.2 g/g protein. Addition of arsenic to the tissues reduced the amount of GSH to 11.5  0.3 g/g protein. But when the arsenic loaded liver tissues were incubated with ascorbic acid at the concentration of 20 g/ml, the amount of GSH was 14.2  0.1 g/g protein. There was 22.6% increase of GSH level which was statistically significant (p<0.001) when compared with arsenic alone. This study suggests that ascorbic acid increased the GSH level in arsenic-treated rat's liver.

  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Quest is still going on for a cheap, effective, and easily available remedy for chronic arsenic toxicity. This study was designed to investigate the effects of folic acid and tetrahydrofolate in lowering the arsenic burden in tissues. Rats received arsenic at 700 µg/day by gavage for 28 days except the control group. Arsenic accumulation was significantly lowered (p<0.05), in liver, kidney, heart, lung and skin in both the folic acid and tetrahydrofolate-treated groups compared to arsenic only treated group. The oxidative stress induced by arsenic treatment was reduced as evident by the reduction in rise of malondialdehyde level in both groups. But folic acid was found to be more efficacious compared to tetrahydrofolate.
    Bangladesh Journal of Pharmacology 03/2010; 5(1). DOI:10.3329/bjp.v5i1.4909 · 0.51 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: The present study was undertaken with a view to evaluate the efficacy of spinach against arsenic (As) induced toxicity in rats during the period between July to October 2008. Thirty six female Long Evans rats (age about 120days; average body weight at day 0 = 154.5g) were randomly divided into three equal groups (n=12) and marked as T 0, T 1 and T 2 groups. Rats of T 0 group were given normal feed and water and kept as control. Rats of T 1 and T 2 groups were given 5mg Sodium arsenite/kg body weight (BW) and 5mg Sodium arsenite/kg (BW) plus spinach extract 100 mg/kg body weight respectively daily for 30 days orally. Four rats from each group were sacrificed at 10 days interval in order to quantitatively determine the As content in liver, lungs and kidney by using Hydride Generation Atomic Absorption Spectrophotometer. Serum glutamate oxaloacetate transaminase (SGOT), serum glutamate pyruvate transaminase (SGPT) and serum creatinine were determined by Autoanalyser. No visible clinical sign were observed in any group of experimental rats except loss of body weight in the spinach treated group. Tissue (lung, liver and kidney) concentration of As was significantly (p<0.01) higher in T 1 group rats compared to that of T 0 and T 2 groups and the highest concentration of As was found in kidney followed by lung and liver in T 1 group rats. After 30 days of feeding, spinach significantly (p<0.01) decreased As from lung, liver and kidney. As intoxication significantly (p<0.01) increased SGOT values but insignificantly decrease SGPT values and spinach treatment improve these condition. There was no significant effect found in serum creatinine level. It can be concluded that feeding of spinach could reduce body burden of As in rats.
    Bangladesh Journal of Veterinary Medicine 09/2010; 7(2). DOI:10.3329/bjvm.v7i2.6005
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: The effects of sodium arsenite exposure on the hepatic maturation period of cellular and functional reorganization in developing rat livers were evaluated. Animals received intraperitoneal injections of sodium arsenite (1.5 mg/kg body weight) or distilled water on days 9 to 28 after birth. On day 29, the animals were sacrificed either by cervical dislocation or by perfusion fixation. The perfusion fixed liver tissue was processed for paraffin embedding, sectioning and hematoxylin and eosin staining. The fresh liver tissue was processed for cryo-sectioning followed by Sudan Black B staining and for biochemical estimation of reduced glutathione. Microscopic observation revealed comparable preserved hepatic lobular patterns and distributions of uninucleate and binucleate hepatocytes in the control and the experimental groups. The mean nuclear area and diameter of the hepatocytes was increased in the experimental group. Lipid droplet distribution pattern in Sudan Black B stained sections revealed higher staining intensity towards the centrilobular area in both groups. Semiquantitative estimation of staining intensity showed lower mean gray values in zone 3 than in zones 2 and 1 (suggestive of the setting in of the adult pattern) in both groups. The reduced glutathione levels in the liver tissue and the altered nuclear size of the hepatocytes in the experimental group suggested the impairment of morphological and biochemical processes induced by arsenic exposure during the postnatal period.
    Anatomy & cell biology 12/2012; 45(4):229-40. DOI:10.5115/acb.2012.45.4.229


1 Download
Available from