Nitrogênio e fósforo no crescimento de plantas de ginseng brasileiro [Pfaffia glomerata(Spreng.) Pedersen] cultivadas in vitro

Ciência Rural (Impact Factor: 0.38). 02/2003; 33(1). DOI: 10.1590/S0103-84782003000100009
Source: DOAJ


O ginseng brasileiro [Pfaffia glomerata (Spreng.) Pedersen] apresenta propriedades medicinais marcantes e, por isso, atualmente, é largamente explorado de forma extrativista, tanto por parte dos coletores de ervas como pela indústria farmacêutica. Este trabalho objetivou caracterizar o efeito da variação isolada da concentração de N e P do meio MS no crescimento de plantas de P. glomerata cultivadas in vitro. Segmentos nodais de 1,0cm de comprimento e sem folhas, de plantas já estabelecidas in vitro, foram cultivados em meio MS contendo cinco concentrações (0, 25, 50, 100 e 150% da concentração padrão do meio de cultura MS) de nitrogênio ou fósforo. Aos 15 dias após a inoculação (DAI), o número de raízes e o percentual de enraizamento são maiores na concentração de N e P equivalentes a 50% daquela do meio de cultura MS. Aos 40 DAI, o crescimento em altura das brotações, número de segmentos nodais, índice de área foliar, número de folhas, matéria seca de raízes, da parte aérea e total da planta é maior na concentração de N e P, em média, próxima a 80% daquela do meio de cultura MS.

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    • "This might have affected their growth in height (Fig. 3C), as previously described for the plants of Brazilian ginseng (Pfaffia glomerata Spreng.) where growth was higher at the typical concentration of nitrogen in MS medium and decreased as the organic nitrogen concentration increased (Russowski and Nicoloso 2003). The al deficiency of A. tenella with the treatments used in the present study influenced the fresh biomass of these plants. "
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    ABSTRACT: The aim of the present study was to investigate the influence of tyrosine on the in vitro growth and the production of the betacyanin pigment in Alternanthera philoxeroides and A. tenella. Nodal segments were inoculated in MS medium containing different concentrations of tyrosine (0, 25, 50 and 75 μM), and the number of sprouts and buds, height, root length, fresh matter of shoots and roots and betacyanin content were evaluated. In A. philoxeroides, the highest production of betacyanin (51.30 mg 100 g-1 FM) was in the stems with the addition of approximately 45 μM tyrosine, while the increase in the leaves was proportional to the tyrosine concentration, and the best average was obtained with a tyrosine concentration of 75 μM (15.32 mg 100 g-1 FM). Higher tyrosine concentrations were deleterious to the growth of A. tenella plants, and a concentration of 75 μM was considered toxic. However, a tyrosine concentration of 50 μM benefitted betacyanin production, which reached 36.5 mg 100 g-1 FM in the plant shoots. These results showed the positive effect of tyrosine on the production of betacyanin in both species; however, application at high concentrations hampered the growth of Alternanthera plants.
    Brazilian Archives of Biology and Technology 12/2012; 57(AHEAD). DOI:10.1590/S1516-89132013005000012 · 0.55 Impact Factor
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    • "In vitro propagation is a promising alternative for providing a regular supply of high quality, disease-free and homogeneous plant material within a shorter period of time (Mosaleeyanon et al., 2004). A number of studies have evaluated factors that can affect the in vitro development of P. glomerata (Nicoloso et al. 2001, 2003; Russowski and Nicoloso 2003; Skrebsky et al. 2004; Maldaner et al. 2007; Alves et al. 2010; Flores et al. 2010). "
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    ABSTRACT: Pfaffia glomerata (Spreng.) Pedersen is a medicinal species of great interest because it produces the phytoecdysteroid 20-hydroxyecdysone (20E). Generally, because of atypical growing conditions, in vitro propagated plants function less efficiently as autotrophs and have poorly developed morphological structures. This study analyzed the autotrophic potential of P. glomerata propa-gated in vitro and evaluated the influence that this has on 20E biosynthesis. Physiological and structural parameters of plants subjected to heterotrophic, photomixotrophic and photoautotrophic growth conditions were evaluated. Levels of 20E were measured by HPLC. Plants were acclimatized in a mixture of soil, sand and substrate, in a greenhouse. Conditions that provided higher carbon input led to an increase in plant growth, and the presence of sucrose was critical, in closure systems without a gas permeable membrane, for normal anatomical development of the micropropagated plants. The absence of sucrose increased photosynthesis and conditions that enhanced photoauto-trophy induced greater levels of 20E. The increase of 20E levels by the photoautotrophic system offers new prospects for increasing the commercial production of this species, and for studies that could elucidate the biosynthetic path-way of phytoecdysteroids in plants.
    Plant Cell Tissue and Organ Culture 08/2012; 110(2). DOI:10.1007/s11240-012-0145-6 · 2.13 Impact Factor
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    ABSTRACT: The species Pfaffia glomerata (Spreng.) Pedersen, Amaranthaceae, occurs naturally all over Brazil. Its roots are used as tonic, anti-tumor, aphrodisiac and food complement, besides other indications. The species has been drastically harvested in nature, and this fact, summed with the natural habitat destruction and growing economic interest in medicinal plants is threatening the wild populations. A strategy to reduce the ecological pressure of harvesting over P.glomerata populations is to cultivate the species. But prior to the cultivation, it is necessary to develop studies that will help in the domestication process. The aim of this research was to evaluate a population of clones of P. glomerata and its progenies, with estimatives of existing variability for breeding; correlation among relevant features to predict the agricultural potential of individuals; heritability of analyzed features; and the expected progress with these features selection. The agricultural experiments were conducted with 7 clones selected from a cultivated population of CPQBA-UNICAMP, in two sites, with three repetitions, using an experimental design of randomysed blocks. The results could not address the expected conclusions. The clones and the progenies did not show variability during the period of the study. The sexual propagation presents advantages over the vegetative propagation for this species. From this study it is possible to make recommendations regarding the methods used for future agricultural experiments.
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