Article

Determinação de proteínas totais via espectrofometria: vantagens e desvantagens dos métodos existentes

Química Nova (Impact Factor: 0.66). 01/1998; DOI: 10.1590/S0100-40421998000600020
Source: DOAJ

ABSTRACT Spectrophotometric determination of total protein is used in several areas such as clinical analysis, food science and technology, biochemistry, protein chemistry, physiology. Five spectrophotometric methods are mostly used: biuret, Lowry, Bradford, Smith and UV absorption. In this review a general overview of these methods is presented (interferences, applications); other methodologies are also discussed.

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