Article

Reclassification of the members of the genus Tetrathiobacter Ghosh et al. 2005 to the genus Advenella Coenye et al. 2005.

Department Sanidad Animal, Facultad de Veterinaria, Universidad Complutense, 28040 Madrid, Spain.
International journal of systematic and evolutionary microbiology (Impact Factor: 2.11). 07/2009; 59(Pt 8):1914-8. DOI: 10.1099/ijs.0.007443-0
Source: PubMed

ABSTRACT The taxonomic position of the genera Advenella and Tetrathiobacter was examined. 16S rRNA gene sequence analysis revealed that the two genera are closely related, representing a monophyletic cluster with high sequence similarity (98.1-99.7%) within the family Alcaligenaceae. The phenotypic characteristics of the type strains of Advenella incenata, Tetrathiobacter kashmirensis and Tetrathiobacter mimigardefordensis were re-examined using the API 20NE, API ZYM and API 50CH systems. Phylogenetic data together with similarities in phenotypic characteristics, G+C content and cellular acid composition suggest that they should be classified in the same genus. On the basis of the data presented, the two species of the genus Tetrathiobacter should be transferred to the genus Advenella, since this genus has nomenclatural priority. Therefore, Tetrathiobacter kashmirensis and Tetrathiobacter mimigardefordensis should be transferred to the genus Advenella as Advenella kashmirensis comb. nov. (type strain WT001T=LMG 22695T=MTCC7002T) and Advenella mimigardefordensis comb. nov. (type strain DPN7T=DSM 17166T=LMG 22922T). Emended descriptions of Advenella incenata and the genus Advenella are also presented.

0 Bookmarks
 · 
160 Views
  • [Show abstract] [Hide abstract]
    ABSTRACT: Advenella mimigardefordensis strain DPN7(T) is a remarkable β-proteobacterium because of its extraordinary ability to use the synthetic disulfide 3,3'-dithiodipropionic acid (DTDP) as sole carbon source and electron donor for aerobic growth. One application of DTDP is as a precursor substrate for biotechnically synthesized polythioesters (PTE), which are interesting non-degradable biopolymers applicable for plastics materials. Metabolic engineering for optimization of PTE-production requires an understanding of DTDP conversion. The genome of A. mimigardefordensis strain DPN7(T) was sequenced and annotated. The circular chromosome is composed of 4,740,516 bp and 4,112 predicted open reading frames, whereas the circular plasmid consists of 23,610 bp and 24 predicted open reading frames. The genes participating in DTDP catabolism have been characterized in detail previously, but knowing the complete genome sequence and with support of Tn5::mob-induced mutants, also putatively involved transporter proteins and a transcriptional regulator were identified. Most probably, DTDP is transported into the cell by a specific tripartite tricarboxylate transport system and is afterwards cleaved by the disulfide-reductase LpdA, sulfoxygenated by the 3-mercaptopropionate dioxygenase Mdo, activated by the CoA-ligase SucCD and desulfinated by the acyl-CoA dehydrogenase-like desulfinase AcdA. Regulation of this pathway is presumably performed by a transcriptional regulator of the xenobiotic response element family. The excessive sulfate that is inevitably produced is secreted by the cells by a unique sulfate exporter of the CPA (cation:proton antiporter) superfamily.
    Microbiology 04/2014; · 3.06 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: A Gram negative, non-motile, non- spore-forming, coccoid bacterium was isolated from stool sample of healthy human subject and formed cream colour colonies on tryptic soy agar. Almost complete length (1500 bp) small subunit ribosomal RNA (16S rRNA) gene sequence was generated and similarity search was conducted by BLAST. Result of similarity search indicated that bacterium belongs to [beta]- subclass of Proteobacteria of family Alacaligenaceae. It showed maximum sequence similarity (96.5%) with Pelistega europea CCUG 39967T followed by Advenella mimigardefordensis DSM 17166T (96.1%) and Taylorella assinigenitalis LMG 19572T (95.15%). DNA G+C content of strain HM-7T was 42 mol%. Strain HM-7T contained 14:0 (4.2%), 16:0 (18.6%), 16:0 3 OH (3.0%), and 18:0 (1.7%) as dominant fatty acids. Morphological, physiological and biochemical data also showed that strain HM-7T belongs to genus Pelistega, but at the same time it delineates with Pelistega europaea CCUG 39967T the only existing species of the genus. Based on polyphasic characterization we concluded that bacterium is a new species of genus Pelistega and proposed as Pelistega indica sp. nov. with strain HM-7T as type strain of the species (= MCC 2184T = DSM 27484T).
    INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 01/2014; · 2.11 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: "Vorarlberger Bergkäse" (VB) is an Austrian artisanal hard cheese produced from raw cow's milk. The composition of its rind microbiota and the changes in the microbial communities during ripening have not previously been investigated. This study used 16S and 18S rRNA gene cloning and sequencing to characterize the bacterial and fungal communities of seven pooled cheese rind samples taken in seven different ripening cellars of three Austrian dairy facilities. A total of 408 clones for 16S and 322 clones for 18S rRNA gene libraries were used for taxonomic classification, revealing 39 bacterial and seven fungal operational taxonomic units (OTUs). Bacterial OTUs belonged to four different phyla. Most OTUs were affiliated to genera often found in cheese, including high numbers of coryneforms. The most abundant OTU from 16S rRNA gene libraries showed highest similarity to Halomonas. Young cheese rinds were dominated by Actinobacteria or Proteobacteria, particularly by Halomonas and Brevibacterium aurantiacum, while Staphyloccocus equorum was most abundant in old cheeses. The most abundant 18S rRNA OTU had highest similarity to the filamentous fungus Scopulariopsis brevicaulis. Pairwise correlation analyses revealed putative co-occurrences between a number of OTUs. It was possible to discriminate the different cheese rind microbiota at the community-level by facility affiliation and ripening time. This work provides insights into the microbial composition of VB cheese rinds and might allow the processing- and ripening conditions to be improved to enhance the quality of the product.
    International journal of food microbiology 04/2014; 180C:88-97. · 3.01 Impact Factor

Full-text

Download
70 Downloads
Available from
May 16, 2014