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Protective effect of Carica papaya L leaf extract against alcohol induced acute gastric damage and blood oxidative stress in rats. West Indian Med J

Department of Molecular Medicine, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia.
The West Indian medical journal (Impact Factor: 0.28). 10/2008; 57(4):323-6.
Source: PubMed

ABSTRACT The effects of Carica papaya leaf (CPL) aqueous extract on alcohol induced acute gastric damage and the immediate blood oxidative stress level were studied in rats. The results showed that gastric ulcer index was significantly reduced in rats pretreated with CPL extract as compared with alcohol treated controls. The in vitro studies using 2,2-Diphenyl-1-Picryl-Hydrazyl (DPPH) assay showed strong antioxidant nature of CPL extract. Biochemical analysis indicated that the acute alcohol induced damage is reflected in the alterations of blood oxidative indices and CPL extract offered some protection with reduction in plasma lipid peroxidation level and increased erythrocyte glutathione peroxidase activity. Carica papaya leaf may potentially serve as a good therapeutic agent for protection against gastric ulcer and oxidative stress.

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    • "It was reported that experimentally induced diabetic rats showed significant wound healing after treatment with the aqueous extract of the fruit of C. papaya [5]. The aqueous extract of the leaves of the plant showed significant protective effect against alcohol induced oxidative damage to the gastric mucosa in rats [6], and it was effective in healing induced wound in rats [7]. The aqueous extract of the leaves also exhibited the ability to inhibit tumor cell lines [8] and this activity was potentiated with phenolic compounds [9] [10]. "
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    ABSTRACT: The subchronic toxicity effect of the leaf extract of Carica papaya Linn. in Sprague Dawley (SD) rats was investigated in this study. The extract was prepared by dissolving the freeze dried extract of the leaves in distilled water and was administered orally to SD rats (consisted of 10 rats/sex/group) at 0 (control), 0.01, 0.14, and 2 g/kg body weight (BW) for 13 weeks. General observation,mortality, and food and water intake were monitored throughout the experimental period. Hematological and biochemical parameters, relative organ weights, and histopathological changes were evaluated.The study showed that leaf extract when administered for 13 weeks did not cause any mortality and abnormalities of behavior or changes in body weight as well as food and water intake.There were no significant differences observed in hematology parameters between treatment and control groups; however significant differences were seen in biochemistry values, for example, LDH, creatinine, total protein, and albumin. However, these changes were not associated with histopathological changes. In conclusion, the results suggested that daily oral administration of rats with C. papaya leaf extract for 13 weeks at a dose up to fourteen times the levels employed in traditional medicine practice did not cause any significant toxic effect.
    Evidence-based Complementary and Alternative Medicine 10/2014; 2014(2014):10 pages. DOI:10.1155/2014/741470 · 1.88 Impact Factor
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    • "The studies of the antioxidant nutrients in C. papaya have led to the identification of the main compounds that differ in the different organs. Whole fruit extract contains ferulic, p-coumaric, and caffeic acid, carotenoids, and vitamin C that collectively protect human cells from oxidative stresses [4] and promote wound healing and skin repair [8, 9]; leaves extract contains folic acid, vitamins B12, A, and C, alkaloids, saponins, glycosides, tannins, and flavonoids [10] with anticancer activity [11, 12] and protection against the alcohol-induced oxidative damage to the gastric mucosa [13]; seeds extract contains different phenolic compounds, vanillic acid, and vitamin C with antioxidant [14–17] and anticancer [5] activities. Thus, C. papaya extracts may act as a synergistic therapeutic dietary supplement in patients with oxidative stress related diseases or could be added to formulations to promote wound healing. "
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    ABSTRACT: Carica papaya is widely used in folk medicine as herbal remedy to prevent, protect against, and cure several diseases. These curative properties are based on the presence in different parts of the plant of phytochemical nutrients with antioxidant effect. Seeds are the less exploited part; thus this study is aimed at assessing the antioxidant activities of the C. papaya seeds water extract against hydrogen peroxide (H2O2) oxidative stress in human skin Detroit 550 fibroblasts. C. papaya seeds water extract is not toxic and acts as a potent free radical scavenger, providing protection to Detroit 550 fibroblasts that underwent H2O2 oxidative stress. Data show that (i) the maximum protective effect is achieved by the simultaneous administration of the extract with 1 mM H2O2; (ii) the extract in presence of an oxidative stress does not increase catalase activity and prevents the release of cytochrome C and the inner mitochondrial transmembrane potential (Δψ m ) loss; (iii) the extract is more efficient than vitamin C to hamper the oxidative damage; (iv) the purified subfractions of the seeds water extract exert the same antioxidant effect of whole extract. In conclusion, C. papaya seeds water extract is potentially useful for protection against oxidative stress.
    Evidence-based Complementary and Alternative Medicine 03/2014; 2014:281508. DOI:10.1155/2014/281508 · 1.88 Impact Factor
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    • "traditional claims have been investigated scientifically using animal model and the efficacy have been proven (Indran et al., 2008; Imaga et al., 2009). Recent studies showed that CP leaf extract has been found to have potential anti sickling (inhibition of sickle cell formation) (Imaga et al., 2009) and has protective effect against gastric ulcer in rats (Indran et al., 2008). CP flowers are known to have antibacterial activities (Zakaria et al., 2006). "
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    ABSTRACT: Carica papaya (CP) leaves are popularly used as food and have many traditional claims for herbal medicine. The leaf extract have been proven scientifically for their efficacy as wound healing and has protective effects against gastric damage in rats. However, toxicity study of the CP leaf extract is still lacking. The present study is to investigate the acute toxicity of CP leaf extract on Sprague Dawley rats at a dose of 2000 mg/kg body weight (BW). Sighting study was conducted in a stepwise procedure using the fixed doses of 5, 50, 300 and 2000 mg/kg BW. No signs of toxicity and no deaths were observed. Based on the sighting study, we selected a dose of 2000 mg/kg BW and were observed for 14 days. The study includes control and treatment group, each consisting of 5 female rats. The single oral dose of the CP leaf extract did not produce mortality or significant changes in the body weight, food and water consumption. The relative weights of the internal organs were normal. However, hemoglobin (HGB), hematocrit (HCT), red blood cell (RBC) and total protein were significantly increase indicating dehydration. Apart from triglyceride, other biochemistry parameters demonstrated no significant changes as compared to the control.
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