Article

In vitro and in vivo evaluation of akermanite bioceramics for bone regeneration.

The Key Laboratory of Stem Cell Biology, Institute of Health Sciences, Chinese Academy of Sciences & Shanghai JiaoTong University School of Medicine, 225 South Chongqing Road, Shanghai 200025, People's Republic of China.
Biomaterials (impact factor: 7.4). 07/2009; 30(28):5041-8. DOI:10.1016/j.biomaterials.2009.05.077 pp.5041-8
Source: PubMed

ABSTRACT This study investigated the effects of a calcium magnesium silicate bioceramic (akermanite) for bone regeneration in vitro and in vivo, with beta-tricalcium phosphate (beta-TCP) as a control. In vitro, the human bone marrow-derived mesenchymal stromal cells (hBMSCs) were cultured in an osteogenic medium supplemented with a certain concentration of two bioceramics' extracts for 20 days. An MTT assay showed that akermanite extract promoted proliferation of hBMSC significantly more than did beta-TCP extract. The results of alkaline phosphatase (ALP) activity test and the expression of osteogenic marker genes such as ALP, osteopontin (OPN), osteocalcin (OCN) and bone sialoprotein (BSP) demonstrated that the osteogenic differentiation of hBMSC was enhanced more by akermanite extract than by beta-TCP extract. In vivo, a histomorphology analysis and histomorphometry of the two porous bioceramics implants in rabbit femur defect models indicated that both in early- and late-stage implantations, akermanite promoted more osteogenesis and biodegradation than did beta-TCP; and in late-stage implantations, the rate of new bone formation was faster in akermanite than in beta-TCP. These results suggest that akermanite might be a potential and attractive bioceramic for tissue engineering.

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    Article: Evaluation of the in vitro bioactivity of bioceramics
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Keywords

akermanite
 
alkaline phosphatase
 
ALP
 
attractive bioceramic
 
beta-tricalcium phosphate
 
bone sialoprotein
 
calcium magnesium silicate bioceramic
 
certain concentration
 
histomorphology analysis
 
human bone marrow-derived mesenchymal stromal cells
 
late-stage implantations
 
MTT assay
 
new bone formation
 
OPN
 
osteogenesis
 
osteogenic differentiation
 
osteogenic marker genes
 
osteogenic medium supplemented
 
tissue engineering
 
two porous bioceramics implants