Improvements of cellular stress response on resveratrol in liposomes

Department of Pharmacy, University of Ljubljana, Askerceva7, Ljubljana 1000, Slovenia.
European journal of pharmaceutics and biopharmaceutics: official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V (Impact Factor: 3.38). 07/2009; 73(2):253-9. DOI: 10.1016/j.ejpb.2009.06.006
Source: PubMed

ABSTRACT Resveratrol (RSV) has proven potential in prophylaxis and treatment of various disorders mediated by free radicals and oxidative stress. RSV solubility, stability, and cytotoxicity must be regulated for satisfactory bioavailability. Here, RSV was loaded into liposomes, characterized by PCS and TEM and evaluated on HEK293 cell line by metabolic activity assay, electron paramagnetic resonance, and fluorescence microscopy. RSV at 10 microM induced changes in cell metabolic activity and significantly improved antioxidative capacity. At 100 microM it showed concentration-dependent cytotoxicity. Oligolamellar liposomes with mean diameter 84 nm, polydispersity index 0.2, and zeta potential -40 mV showed high entrapment of RSV and rapid cellular internalization. Cell stress caused by UV-B irradiation diminished cell metabolic activity by 50%. RSV loaded into them showed no cytotoxicity at 100 microM and stimulated cellular metabolic and antioxidant activity levels to eliminate the harmful effect of the stress. Localization of RSV within liposomal bilayer is crucial for stimulation of cell-defense system, prevention of RSV cytotoxicity, and its long-term stability. In summary, evidence of different metabolic activity using free RSV and LIP-RSV is presented indicating that liposome-mediated uptake of RSV is more effective for improvement of the cell-stress response.

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Available from: Marjeta Sentjurc, Sep 28, 2015
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    • "Polyphenolic compounds are widely distributed in medicinal plants, vegetables, fruit and have received considerable attention during the past years as they possess many beneficial effects on human health (Caddeo et al., 2008; Sinico et al., 2008; Kristl et al., 2009; Caddeo et al., 2013). In particular, quercetin (QUE; 3,3 0 ,4 0 ,5,7-pentahydroxyflavone) has been demonstrated to exert strong antioxidant and anti-inflammatory activities against osteoporosis, certain forms of cancer, pulmonary and cardiovascular diseases and aging (Boots et al., 2008). "
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    Journal of Microencapsulation 06/2014; 31(7):1-6. DOI:10.3109/02652048.2014.913726 · 1.59 Impact Factor
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    01/2014; 2014:1-6. DOI:10.1155/2014/248635
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