A recombinant-based ELISA evaluating the efficacy of netobimin and albendazole in ruminants with naturally acquired fascioliasis

Animal Pathology Department, Epidemiology, Zoonoses and Parasitic Diseases, Veterinary Faculty, Santiago de Compostela University, 27002 Lugo, Spain.
The Veterinary Journal (Impact Factor: 1.76). 11/2009; 182(1):73-8. DOI: 10.1016/j.tvjl.2008.05.015
Source: PubMed


The therapeutic efficacy of albendazole and netobimin in ruminants with naturally occurring fascioliasis was investigated using a recombinant-based ELISA. The variation in the IgG response against a 2.9-kDa recombinant protein (FhrAPS), termed efficacy index (EI) 1, and the egg-output changes, termed EI 2, were used to evaluate drug efficacy. The values of EI 1 ranged between 0% and 50% in sheep, and between 0% and 30% in cattle after treatment with albendazole and netobimin. Similar EI 2 values were observed in sheep receiving albendazole or netobimin, but the highest values were found in cattle treated with netobimin. The significant reduction in the IgG response to FhrAPS found in this study shows promise in terms of developing alternative methods for evaluating the efficacy of chemotherapy against Fasciola hepatica in grazing ruminants.

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    • "En la estimación de la prevalencia basada en la detección de anticuerpos debemos tener en cuenta que los hospedadores son tratados con fármacos antiparasitarios . Aunque los valores de anticuerpos se reducen significativamente en los rumiantes que reciben albendazol o netobimin, no se llegan a registrar valores correspondientes a animales no infectados (Arias y col 2009). En ambos países, el ganado vacuno recibe dos dosis de tratamiento fasciolicida, en primavera y en otoño. "

    Archivos de Medicina Veterinaria 01/2015; 47(2):201-208. DOI:10.4067/S0301-732X2015000200011 · 0.31 Impact Factor
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    • "These results are in coincidence with that reported previously (Phiri et al., 2006; Yabe et al., 2008; Paz-Silva et al., 2010). Thirdly, the demonstration of elevated levels of antibodies for long periods in healthy animals, even after the administration of successful chemotherapy (Espino et al., 1998; Paz-Silva et al., 2003; Arias et al., 2007) does not allow discriminating between current and past infections, and ELISA-positive results should be associated to exposure to the trematodes and/or their antigens (Arias et al., 2009). Despite the difference in bovine management, some analogies can be underlined in the two regions. "
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    ABSTRACT: The analysis of infection by Paramphistomidae trematodes was conducted in two agricultural regions with different knowledge on this parasitosis. Faecal and blood samples were collected from 374 cattle in Salto (NW Uruguay) where there is a lack of information about paramphistomosis. A total of 429 cattle from Galicia (NW Spain), an area with previous records of infection by gastric flukes, were sampled. Diagnostics of trematodosis was developed by using a copromicroscopic probe and an ELISA with excretory/secretory antigens collected from adult Calicophoron daubneyi (Paramphistomidae) specimens. Results were evaluated according intrinsic and extrinsic factors. In the Uruguay, the percentage of cattle passing Paramphistomidae-eggs by faeces was 7% (95% Confidence Interval 5, 10). A significantly higher prevalence of paramphistomosis in the Hereford×Angus cattle (OR=3.5) was recorded, as observed for the oldest ruminants (>3.5years). An overall seroprevalence of 29% (25, 34) was obtained by ELISA, with the highest values in the Friesians (OR=3), the youngest bovines (<2.5years) and dairy cattle (Friesians). Twenty-six percent (22, 30) of the cattle from Spain passed eggs by faeces, and cattle aged 2.5-7years reached significant highest prevalences. By means of the ELISA, a percentage of 55 cattle (50, 59) had antibodies against the gastric fluke, and the highest seroprevalence was observed among the bovines under 6years. It is concluded that paramphistomosis is on the increase in cattle from NW Spain, partly due to the absence of an effective treatment against the trematode. There is a need for reducing the risk of infection by Paramhistomidae spp. in cattle from Uruguay, especially by improving their management to avoid exposure to the gastric trematode. Further studies are in progress for identifying the species of Paramphistomidae affecting ruminants in Uruguay.
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    ABSTRACT: A cross-sectional study was conducted to assess the seroprevalence of fascioliasis by immunoenzymatic probes in an endemic area (northwestern Spain). Blood samples were collected from 1,034 cattle (crossbred, Rubia Gallega, and Friesian breeds), and the diagnosis of fascioliasis was carried out by determining both the occurrence of antigenemia and the presence of specific IgG antibodies against a Fasciola hepatica recombinant protein (FhrAPS). The IgG seroprevalence was 65% (95% CI, 62-68) by the FhrAPS-ELISA, and 32% (29-35) exhibited antigenemia; the lowest percentages occurred in the Friesians, and the highest percentages were found in the crossbreds. These results confirm an elevated seroprevalence of fascioliasis that is unexpected considering that most of the cattle livestock (Friesian and Rubia Gallega) receive fasciolicide treatment. The lack of adequate measures on the environment and erratic chemotherapy seem to be responsible for the fact that control of fascioliasis has not improved in the last 10 yr in the area of study.
    Journal of Parasitology 11/2009; 96(3):626-31. DOI:10.1645/GE-2333.1 · 1.23 Impact Factor
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