Article

Chemotactic Behavior of Pathogenic and Nonpathogenic Leptospira Species

Institut Pasteur, Unité de Biologie des Spirochètes, Paris, France.
Applied and Environmental Microbiology (Impact Factor: 3.95). 12/2012; 78(23). DOI: 10.1128/AEM.02288-12

ABSTRACT We have developed a capillary tube assay in combination with real-time PCR to quantitate the number of chemoattracted Leptospira cells. We identified Tween 80, glucose, sucrose, and pyruvate as attractants for Leptospira cells; amino acids and vitamin B12 were found to be nonchemotactic or weakly chemotactic. This assay has the general applicability to further our understanding of leptospiral chemotaxis.

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