Article

Human eosinophil cationic protein enhances stress fiber formation in Balb/c 3T3 fibroblasts and differentiation of rat neonatal cardiomyocytes.

Department of Medical and Bioengineering Science, Graduate School of Natural Science and Technology, Okayama University, Okayama, Japan.
Growth factors (Chur, Switzerland) (impact factor: 2.47). 07/2009; 27(4):228-36. DOI:10.1080/08977190902987149 pp.228-36
Source: PubMed

ABSTRACT We found that eosinophil cationic protein (ECP) stimulated the growth of mouse Balb/c 3T3 fibroblasts. ECP-treated 3T3 cells were more flattened and exhibited enhanced stress fiber formation. The enhancement of cytoskeleton after addition of recombinant ECP appeared stable and was able to inhibit disassembly of actin filaments that was induced by fibroblast growth factor-2. The ROCK inhibitor, Y-27632, abrogated this enhancement on stress fiber formation that was induced by ECP indicating the involvement of Rho/ROCK signaling pathway. The effect of ECP was assessed on the differentiation of primary cardiomyocytes derived from rat neonatal heart since the development of actin filaments is significantly related with organization of stress fibers. As the result, both beating rate and the expression of cardiac muscle specific markers such as atrial natriuretic factor were enhanced in the presence of ECP. Thus ECP may also function as a cardiomyocyte differentiation factor.

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Keywords

able
 
actin filaments
 
atrial natriuretic factor
 
cardiac muscle specific markers
 
cardiomyocyte differentiation factor
 
ECP-treated 3T3 cells
 
eosinophil cationic protein
 
fibroblast growth factor-2
 
mouse Balb/c 3T3 fibroblasts
 
primary cardiomyocytes
 
rat neonatal heart
 
Rho/ROCK signaling pathway
 
stable
 
stress fiber formation
 
stress fibers