Development and Maintenance of a Biospecimen Repository for Clinical Samples Derived from Pulmonary Patients
ABSTRACT The Pulmonary Biospecimen Repository (PBR) at the University of Alabama at Birmingham (UAB) was launched in 2009. The purpose of the UAB PBR is to provide investigators within the pulmonary community at UAB and elsewhere with clinical samples derived from multiple lung diseases, including transplant recipients, idiopathic pulmonary fibrosis, chronic obstructive pulmonary disease, cystic fibrosis, and asthma. Cell and fluid samples isolated from bronchoalveolar lavage (BAL), plasma, and serum are collected and stored; samples are assessed routinely for viability. Each sample is linked directly with the respective patient information via the Pulmonary Translational Research and Clinical Database, a Health Insurance Portability and Accountability Act compliant database that includes detailed information allowing for the study of specific patient cohorts. To access samples, investigators must complete a request form, which is reviewed by the UAB PBR Steering Committee. To date, more than 800 patients have provided approximately 7,000 BAL, serum and plasma fluid, and cell samples. Over the past 4 years, nearly 800 of these samples have been distributed to investigators at UAB and elsewhere. Future plans for the UAB PBR include expanding sample collection to additional pulmonary diseases, such as mycobacterial infections, increasing the number of sample users and obtaining external funding to ensure its continued sustainability.
SourceAvailable from: Jeffrey C Horowitz[Show abstract] [Hide abstract]
ABSTRACT: The median survival of patients with idiopathic pulmonary fibrosis continues to be approximately 3 years from the time of diagnosis, underscoring the lack of effective medical therapies for this disease. In the United States alone, approximately 40,000 patients die of this disease annually. In November 2012, the National Heart, Lung and Blood Institute held a workshop aimed at coordinating research efforts and accelerating the development of idiopathic pulmonary fibrosis therapies. Basic, translational and clinical researchers gathered with representatives from the National Heart Lung and Blood Institute, patient advocacy groups, pharmaceutical companies and the Food and Drug Administration to review the current state of idiopathic pulmonary fibrosis research and identify priority areas, opportunities for collaborations and directions for future research. The workshop was organized into groups that were tasked with assessing and making recommendations to promote progress in one of the following six critical areas of research: 1) biology of alveolar epithelial injury and aberrant repair, 2) role of extracellular matrix, 3) preclinical modeling, 4) the role of inflammation and immunity, 5) genetic, epigenetic and environmental determinants, 6) translation of discoveries into diagnostics and therapeutics. The workshop recommendations provide a basis for directing future research and strategic planning by scientific, professional and patient communities and the National Heart Lung and Blood Institute.American Journal of Respiratory and Critical Care Medicine 10/2013; DOI:10.1164/rccm.201306-1141WS · 11.04 Impact Factor
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ABSTRACT: The purpose of this analysis was to examine potential associations between absence, extended (repeated) absence, tardiness, and repeated tardiness from school and doctor-diagnosed asthma. A cross-sectional analysis was conducted using data collected in fall 2007 on 914 4th and 5th grade school children from seven randomly selected participating schools in DeKalb County, GA. ANOVA was used to compare attendance metrics for race, gender, and asthma status groups. Nonparametric Kruskal-Wallis statistical tests were also done because distributions of attendance metrics were skewed. We tallied daily absence data during the study period and examined absence by day of week. We also compared the variation of the weekly rate of absence and tardiness for students with asthma versus students without asthma over the study period. The mean days of absence in 86 students with asthma was 2.73 days compared with 1.89 days for 828 children without asthma (p = .004). There was no significant difference in mean days of tardiness by asthma status. The difference in the number of instances of 2+ consecutive days of absence (extended absence) by asthma status was not significant. Students with asthma were more likely to be absent on Mondays (p = .005), Tuesdays (p = .001), and Fridays (p = .02) than students without asthma. The weekly rate of tardiness for students with asthma trended with the general student study population over the study period, whereas the weekly rate of absence did not. Asthma was associated with increased 1-day absences but not longer absences or tardiness.Journal of Asthma 02/2011; 48(3):228-34. DOI:10.3109/02770903.2011.555038 · 1.83 Impact Factor
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ABSTRACT: The contribution of nitric oxide (NO) to the pathophysiology of asthma remains incompletely defined despite its established pro- and anti-inflammatory effects. Induction of the inducible nitric oxide synthase (iNOS), arginase, and superoxide pathways is correlated with increased airway hyperresponsiveness in asthmatic subjects. To determine the contributions of these pathways in proximal and distal airways, we compared bronchial wash (BW) to traditional bronchoalveolar lavage (BAL) for measurements of reactive nitrogen/oxygen species, arginase activation, and cytokine/chemokine levels in asthmatic and normal subjects. Levels of NO were preferentially elevated in the BAL, demonstrating higher level NOS activation in the distal airway compartment of asthmatic subjects. In contrast, DHE(+) cells, which have the potential to generate reactive oxygen species, were increased in both proximal and distal airway compartments of asthmatics compared to controls. Different patterns of cytokines and chemokines were observed, with a predominance of epithelial cell-associated mediators in the BW compared to macrophage/monocyte-derived mediators in the BAL of asthmatic subjects. Our study demonstrates differential production of reactive species and soluble mediators within the distal airways compared to the proximal airways in asthma. These results indicate that cellular mechanisms are activated in the distal airways of asthmatics and must be considered in the development of therapeutic strategies for this chronic inflammatory disorder.Free Radical Biology and Medicine 03/2011; 50(11):1679-88. DOI:10.1016/j.freeradbiomed.2011.03.015 · 5.27 Impact Factor