Bacillus solisalsi sp. nov., a halotolerant, alkaliphilic bacterium isolated from soil around a salt lake.
ABSTRACT A novel Gram-positive, motile, rod-shaped bacterium isolated from a saline soil in China was characterized by a polyphasic taxonomic approach. The strain, designated YC1(T), was halotolerant [tolerating up to 15 % (w/v) NaCl] and alkaliphilic (growing at a broad pH range of 5-13). 16S rRNA gene sequence analysis revealed that the isolate belonged to the genus Bacillus, showing highest similarity to Bacillus macauensis JCM 13285(T) (98.0 %). However, DNA-DNA hybridization indicated low levels of genomic relatedness with B. macauensis JCM 13285(T) (8.5 %). The major isoprenoid quinone was MK-7 and the cellular fatty acid profile consisted of significant amounts of iso-C(15 : 0) (38.6 %) and anteiso-C(15 : 0) (35.9 %). The predominant polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The G+C content of the genomic DNA was 41.8 mol%. On the basis of the polyphasic evidence from this study, strain YC1(T) (=KCTC 13181(T)=CGMCC 1.6854(T)) should be classified as the type strain of a novel species of the genus Bacillus, for which the name Bacillus solisalsi sp. nov. is proposed.
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ABSTRACT: An ad hoc committee for the re-evaluation of the species definition in bacteriology met in Gent, Belgium, in February 2002. The committee made various recommendations regarding the species definition in the light of developments in methodologies available to systematists.International journal of systematic and evolutionary microbiology 06/2002; 52(Pt 3):1043-7. · 2.11 Impact Factor
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ABSTRACT: A halophilic bacterium, strain BH030017(T), showing optimum growth at 2-5 % (w/v) NaCl was isolated from solar-saltern sediment from the Tae-An area of Korea and was characterized taxonomically. The cells of strain BH030017(T) were Gram-positive, motile, short rods containing cell-wall peptidoglycan based on meso-diaminopimelic acid. The major cellular fatty acids were anteiso-C(15 : 0) and iso-C(15 : 0). The DNA G+C content was 36 mol% and the predominant lipoquinone was MK-7. The major cellular phospholipids were phosphatidylglycerol, diphosphatidylglycerol and phosphatidylethanolamine. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain BH030017(T) formed a cluster with Bacillus clarkii DSM 8720(T) and Bacillus agaradhaerens DSM 8721(T) within the family Bacillaceae. 16S rRNA gene sequence similarities with respect to closely related type strains were less than 95.1 %. On the basis of its phylogenetic, phenotypic and chemotaxonomic properties, strain BH030017(T) represents a novel species within the genus Bacillus, for which the name Bacillus taeanensis sp. nov. is proposed. The type strain is BH030017(T) (=KCTC 3918(T)=DSM 16466(T)).International journal of systematic and evolutionary microbiology 01/2007; 56(Pt 12):2903-8. · 2.11 Impact Factor
- European Journal of Biochemistry 12/1967; 2(4):448-53. · 3.58 Impact Factor