Bacillus solisalsi sp. nov., a halotolerant, alkaliphilic bacterium isolated from soil around a salt lake.

Biotoxin Units of Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, Yunnan, PR China.
International journal of systematic and evolutionary microbiology (Impact Factor: 2.11). 07/2009; 59(Pt 6):1460-4. DOI: 10.1099/ijs.0.000653-0
Source: PubMed

ABSTRACT A novel Gram-positive, motile, rod-shaped bacterium isolated from a saline soil in China was characterized by a polyphasic taxonomic approach. The strain, designated YC1(T), was halotolerant [tolerating up to 15 % (w/v) NaCl] and alkaliphilic (growing at a broad pH range of 5-13). 16S rRNA gene sequence analysis revealed that the isolate belonged to the genus Bacillus, showing highest similarity to Bacillus macauensis JCM 13285(T) (98.0 %). However, DNA-DNA hybridization indicated low levels of genomic relatedness with B. macauensis JCM 13285(T) (8.5 %). The major isoprenoid quinone was MK-7 and the cellular fatty acid profile consisted of significant amounts of iso-C(15 : 0) (38.6 %) and anteiso-C(15 : 0) (35.9 %). The predominant polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The G+C content of the genomic DNA was 41.8 mol%. On the basis of the polyphasic evidence from this study, strain YC1(T) (=KCTC 13181(T)=CGMCC 1.6854(T)) should be classified as the type strain of a novel species of the genus Bacillus, for which the name Bacillus solisalsi sp. nov. is proposed.

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    ABSTRACT: A Gram-positive staining, aerobic endospore forming bacterium (Ca7T) was isolated from a bioreactor showing extensive phosphorus removal. Based on 16S rRNA gene sequence similarity comparisons, strain Ca7T was grouped into the genus Bacillus most closely related to Bacillus nanhaiensis JSM 082006T (100%), Bacillus barbaricus V2-BIII-A2T (99.2%), and Bacillus arsenicus Con a/3T (97.7%). Moderate 16S rRNA gene sequence similarities were found to the type strains of the species B. gelatini and B. rigui (96.4%), B. macauensis (95.1%), and B. solisalsi (96.1%). All these species were grouped into a monophyletic cluster and showed very low sequence similarities (< 94%) to the type species of the genus Bacillus, B. subtilis. The quinone system of strain Ca7T consist predominantly of menaquinone MK-7. The polar lipid profile exhibited the major compounds diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. In addition, minor compounds of an unidentified phospholipid and an aminophospholipid were detected. No glycolipids were found in strain Ca7T, which was consistent with the lipid profiles of B. nanhaiensis, B. barbaricus, B. arsenicus, B. rigui, B. solisalsi, B. macauensis, and B. gelatini, but in contrast to Bacillus subtilis the type species of the genus Bacillus. The diagnostic diamino acid of the peptidoglycan was meso-diaminopimelic acid and the polyamine pattern contained predominantly spermidine and spermine. The major fatty acids, which were C15:0 iso, C15:0 anteiso and C16:0, supported the grouping of strain Ca7T into the family Bacillaceae. The strain showed DNA-DNA similarities of 48% (reciprocal 47%) to B. nanhaiensis DSM 23009T, 31% (reciprocal 36%) to B. barbaricus VS III-A2T, and 29% (reciprocal 39%) to B. arsenicus DSM 15822T, respectively. These results clearly demonstrate that strain Ca7T is a representative of a novel species, which can be differentiated from its closest relative by physiological and biochemical tests. Because of the low sequence similarity of strain Ca7T to Bacillus subtilis, which was shared by B. nanhaiensis, B. barbaricus, B. arsenicus, B. gelatini, B. rigui, B. solisalsi and B. macauensis, and their unique lipid patterns, we propose that strain Ca7T represents a novel species in a novel genus for which the name Fictibacillus phosphorivorans gen. nov. sp. nov. is proposed The type strain is Ca7T (= CCM 8426T = LMG 27063T). In addition we propose the reclassification of B. nanhaiensis, B. barbaricus, B. arsenicus, B. rigui, B. macauensis and B. gelatini as Fictibacillus nanhaiensis comb. nov., Fictibacillus barbaricus comb. nov., Fictibacillus arsenicus comb. nov., Fictibacillus rigui comb. nov. Fictibacillus macauensis comb. nov., and Fictibacillus gelatini comb. nov., respectively.
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    Antonie van Leeuwenhoek 12/2013; · 2.07 Impact Factor