Maribacter antarcticus sp. nov., a psychrophilic bacterium isolated from a culture of the Antarctic green alga Pyramimonas gelidicola.

School of Earth and Environmental Sciences and Research Institute of Oceanography, Seoul National University, San 56-1, Shillim-dong, Kwanak-gu, Seoul 151-742, Republic of Korea.
International journal of systematic and evolutionary microbiology (Impact Factor: 2.11). 07/2009; 59(Pt 6):1455-9. DOI: 10.1099/ijs.0.006056-0
Source: PubMed

ABSTRACT A psychrophilic, Gram-negative, dark orange-pigmented bacterium, designated CL-AP4(T), was isolated from a culture of the green alga Pyramimonas gelidicola obtained from the Southern Ocean. Strain CL-AP4(T) grew optimally at 10 degrees C, in the presence of 3-4 % sea salts and at pH 8. 16S rRNA gene sequence analysis revealed that strain CL-AP4(T) belonged to the family Flavobacteriaceae, with Maribacter arcticus KOPRI 20941(T) as its closest relative (97.2 % similarity). A number of chemotaxonomic characteristics supported affiliation of strain CL-AP4(T) with the genus Maribacter, i.e. iso-C(15 : 0) (17.2 %), iso-C(15 : 1) (16.8 %) and iso-C(17 : 0) 3-OH (14.9 %) were the dominant fatty acids, MK-6 was the major menaquinone and the DNA G+C content was 37.1 mol%. DNA-DNA relatedness between CL-AP4(T) and M. arcticus KOPRI 20941(T) was only 10 %, suggesting that they are genomically distinct species. In addition, strain CL-AP4(T) differed phenotypically from M. arcticus in its optimum growth temperature, its ability to hydrolyse starch, Tween 40 and Tween 80, and production of certain enzymes. On the basis of the results of the polyphasic analysis, strain CL-AP4(T) was classified in the genus Maribacter as belonging to a novel species, for which the name Maribacter antarcticus sp. nov. is proposed; the type strain is CL-AP4(T) (=KCCM 90069(T)=JCM 15445(T)).

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    ABSTRACT: A Gram-staining-negative, strictly aerobic bacterial strain motile by gliding, designated GY20T, was isolated from a tidal flat at Gwangyang bay, South Korea. Cells were moderately halophilic, catalase- and oxidase-positive rods. Growth of strain GY20T was observed at 10-30 °C (optimum, 25 °C), at pH 6.5-11.0 (optimum, pH 7.0-8.0) and in the presence of 1-5 % (w/v) NaCl (optimum, 2-3 %). Only MK-6 was detected as the isoprenoid quinone and iso-C15:0, iso-C15:1 G, summed feature 3 (comprising C16:1 ω7c and/or C16:1 ω6c), summed feature 9 (comprising iso-C17:1 ω9c and/or C16:0 10-methyl), iso-C17:0 3-OH and iso-C15:0 3-OH as major fatty acids. Strain GY20T contained phosphatidylethanolamine, one unidentified glycolipid and one unidentified lipid as major polar lipids. The G+C content of the genomic DNA was 39.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain GY20T formed a tight phyletic lineage with members of the genus Maribacter. Strain GY20T was most closely related to Maribacter dokdonensis DSW-8T with sequence similarity of 96.2 %. On the basis of phenotypic, chemotaxonomic and molecular properties, strain GY20T was shown to represent a novel species within the genus Maribacter, for which the name Maribacter aestuarii sp. nov. is proposed. The type strain is GY20T (=KACC 16440T =JCM 18631T). Emended description of the genus Maribacter is also proposed.
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    ABSTRACT: A Gram-stain-negative, non-spore-forming, strictly aerobic, orange pigmented bacterial strain, motile by gliding, designated CAU 1044T, was isolated from a green seaweed and its taxonomic position was investigated using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CAU 1044T formed a distinct lineage within the genus Maribacter and was most closely related to Maribacter antarcticus JCM 15445T and Maribacter arcticus KOPRI 20941T (96.3 and 95.7% similarity, respectively). Strain CAU 1044T contained MK-6 as the only isoprenoid quinone and iso-C15:0, summed feature 3 (comprising C16:1ω7c and/or C16:1ω6c and/or iso C15:0), iso-C17:0 3-OH, and iso-C15:1 G as the major fatty acids. The cell wall peptidoglycan of strain CAU 1044T contained meso-diaminopimelic acid and the major whole-cell sugars were glucose and ribose. The polar lipids were composed of phosphatidylethanolamine, one unidentified phospholipid, six unidentified aminolipids, and four unidentified lipids. The DNA G+C content was 40.2 mol%. On the basis of phenotypic data and phylogenetic inference, strain CAU 1044T should be classified as a novel species in the genus Maribacter for which the name Maribacter chungangensis sp. nov. is proposed. The type strain is CAU 1044T (KCTC 23735T, CCUG 61948T). Emended descriptions of the genus Maribacter and M. arcticus KCTC 22053T are also proposed.