Maribacter antarcticus sp. nov., a psychrophilic bacterium isolated from a culture of the Antarctic green alga Pyramimonas gelidicola

School of Earth and Environmental Sciences and Research Institute of Oceanography, Seoul National University, San 56-1, Shillim-dong, Kwanak-gu, Seoul 151-742, Republic of Korea.
International Journal of Systematic and Evolutionary Microbiology (Impact Factor: 2.51). 07/2009; 59(Pt 6):1455-9. DOI: 10.1099/ijs.0.006056-0
Source: PubMed

ABSTRACT A psychrophilic, Gram-negative, dark orange-pigmented bacterium, designated CL-AP4(T), was isolated from a culture of the green alga Pyramimonas gelidicola obtained from the Southern Ocean. Strain CL-AP4(T) grew optimally at 10 degrees C, in the presence of 3-4 % sea salts and at pH 8. 16S rRNA gene sequence analysis revealed that strain CL-AP4(T) belonged to the family Flavobacteriaceae, with Maribacter arcticus KOPRI 20941(T) as its closest relative (97.2 % similarity). A number of chemotaxonomic characteristics supported affiliation of strain CL-AP4(T) with the genus Maribacter, i.e. iso-C(15 : 0) (17.2 %), iso-C(15 : 1) (16.8 %) and iso-C(17 : 0) 3-OH (14.9 %) were the dominant fatty acids, MK-6 was the major menaquinone and the DNA G+C content was 37.1 mol%. DNA-DNA relatedness between CL-AP4(T) and M. arcticus KOPRI 20941(T) was only 10 %, suggesting that they are genomically distinct species. In addition, strain CL-AP4(T) differed phenotypically from M. arcticus in its optimum growth temperature, its ability to hydrolyse starch, Tween 40 and Tween 80, and production of certain enzymes. On the basis of the results of the polyphasic analysis, strain CL-AP4(T) was classified in the genus Maribacter as belonging to a novel species, for which the name Maribacter antarcticus sp. nov. is proposed; the type strain is CL-AP4(T) (=KCCM 90069(T)=JCM 15445(T)).

6 Reads
    • ")] and algae [Maribacter ulvicola (Nedashkovskaya et al., 2004), Maribacter polysiphoniae (Nedashkovskaya et al., 2007), Maribacter antarcticus (Zhang et al., 2009), Maribacter stanieri (Nedashkovskaya et al., 2010), Maribacter chungangensis (Weerawongwiwat et al., 2013)]. "
    [Show abstract] [Hide abstract]
    ABSTRACT: Two Gram-staining-negative, rod-shaped, orange-coloured, catalase positive, oxidase positive, non-motile bacteria designated W13M1AT and W15M10T were isolated from the marine sponges Suberites carnosus and Leucosolenia sp. respectively, sampled from Lough Hyne, Co. Cork, Ireland. Analysis of the 16S rRNA gene sequences of these isolates revealed that they are members of the genus Maribacter, in the Flavobacteriaceae family of the phylum Bacteroidetes. The type strain most closely related to strain W13M1AT is M. forsetii DSM 18668T with gene sequence similarity of 96.5%. The closest related type strain to strain W15M10T is M. orientalis DSM 16471T with gene sequence similarity of 98.3%. Phylogenetic inference and phenotypic data combined indicate that strains W13M1AT and W15M10T represent novel species in the genus Maribacter and the names Maribacter vaceletii and Maribacter spongiicola are proposed with W13M1AT (= NCIMB 14724T = DSM 25230T) and W15M10T (= NCIMB 14725T = DSM 25233T), respectively as the type strains.
    International Journal of Systematic and Evolutionary Microbiology 04/2015; 65(7). DOI:10.1099/ijs.0.000224 · 2.51 Impact Factor
  • Source
    • "nov. Bacteroidetes Antarctic sea ice Bowman et al., 2003 Brumimicrobium glaciale Bacteroidetes Antarctic sea ice Bowman et al., 2003 Maribacter arcticus Bacteroidetes Arctic marine sediment Cho et al., 2008 Maribacter antarcticus Bacteroidetes Antarctica, Southern ocean Zhang et al., 2009 Octadecabacter arcticus gen. nov. "
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Strain HTCC2170 was isolated from surface waters off the Oregon coast using dilution-to-extinction culturing. Here, we present the finished genome sequence of a marine bacterium, Maribacter sp. strain HTCC2170. Strain sp. HTCC2170 is predicted to be a facultatively aerobic chemoorganotroph that, based on genomic sequence analysis, is capable of macromolecule degradation and anaerobic respiration.
    Journal of bacteriology 10/2010; 193(1):303-4. DOI:10.1128/JB.01207-10 · 2.81 Impact Factor
Show more