Maribacter antarcticus sp. nov., a psychrophilic bacterium isolated from a culture of the Antarctic green alga Pyramimonas gelidicola

School of Earth and Environmental Sciences and Research Institute of Oceanography, Seoul National University, San 56-1, Shillim-dong, Kwanak-gu, Seoul 151-742, Republic of Korea.
International Journal of Systematic and Evolutionary Microbiology (Impact Factor: 2.51). 07/2009; 59(Pt 6):1455-9. DOI: 10.1099/ijs.0.006056-0
Source: PubMed


A psychrophilic, Gram-negative, dark orange-pigmented bacterium, designated CL-AP4(T), was isolated from a culture of the green alga Pyramimonas gelidicola obtained from the Southern Ocean. Strain CL-AP4(T) grew optimally at 10 degrees C, in the presence of 3-4 % sea salts and at pH 8. 16S rRNA gene sequence analysis revealed that strain CL-AP4(T) belonged to the family Flavobacteriaceae, with Maribacter arcticus KOPRI 20941(T) as its closest relative (97.2 % similarity). A number of chemotaxonomic characteristics supported affiliation of strain CL-AP4(T) with the genus Maribacter, i.e. iso-C(15 : 0) (17.2 %), iso-C(15 : 1) (16.8 %) and iso-C(17 : 0) 3-OH (14.9 %) were the dominant fatty acids, MK-6 was the major menaquinone and the DNA G+C content was 37.1 mol%. DNA-DNA relatedness between CL-AP4(T) and M. arcticus KOPRI 20941(T) was only 10 %, suggesting that they are genomically distinct species. In addition, strain CL-AP4(T) differed phenotypically from M. arcticus in its optimum growth temperature, its ability to hydrolyse starch, Tween 40 and Tween 80, and production of certain enzymes. On the basis of the results of the polyphasic analysis, strain CL-AP4(T) was classified in the genus Maribacter as belonging to a novel species, for which the name Maribacter antarcticus sp. nov. is proposed; the type strain is CL-AP4(T) (=KCCM 90069(T)=JCM 15445(T)).

6 Reads
    • ")] and algae [Maribacter ulvicola (Nedashkovskaya et al., 2004), Maribacter polysiphoniae (Nedashkovskaya et al., 2007), Maribacter antarcticus (Zhang et al., 2009), Maribacter stanieri (Nedashkovskaya et al., 2010), Maribacter chungangensis (Weerawongwiwat et al., 2013)]. "
    [Show abstract] [Hide abstract]
    ABSTRACT: Two Gram-staining-negative, rod-shaped, orange-coloured, catalase positive, oxidase positive, non-motile bacteria designated W13M1AT and W15M10T were isolated from the marine sponges Suberites carnosus and Leucosolenia sp. respectively, sampled from Lough Hyne, Co. Cork, Ireland. Analysis of the 16S rRNA gene sequences of these isolates revealed that they are members of the genus Maribacter, in the Flavobacteriaceae family of the phylum Bacteroidetes. The type strain most closely related to strain W13M1AT is M. forsetii DSM 18668T with gene sequence similarity of 96.5%. The closest related type strain to strain W15M10T is M. orientalis DSM 16471T with gene sequence similarity of 98.3%. Phylogenetic inference and phenotypic data combined indicate that strains W13M1AT and W15M10T represent novel species in the genus Maribacter and the names Maribacter vaceletii and Maribacter spongiicola are proposed with W13M1AT (= NCIMB 14724T = DSM 25230T) and W15M10T (= NCIMB 14725T = DSM 25233T), respectively as the type strains.
    International Journal of Systematic and Evolutionary Microbiology 04/2015; 65(7). DOI:10.1099/ijs.0.000224 · 2.51 Impact Factor
  • Source
    • "Members of the genus Maribacter have been isolated from diverse marine habitats such as green and red algae, seawater and marine sediment (Nedashkovskaya & Kim, 2011). At the time of writing, the genus Maribacter contains 11 species with validly published names: Maribacter aquivivus, M. orientalis, M. sedimenticola, M. ulvicola (Nedashkovskaya et al., 2004), M. dokdonensis (Yoon et al., 2005), M. polysiphoniae (Nedashkovskaya et al., 2007), M. forsetii (Barbeyron et al., 2008), M. arcticus (Cho et al., 2008), M. antarcticus (Zhang et al., 2009), M. stanieri (Nedashkovskaya et al., 2010) and M. chungangensis (Weerawongwiwat et al., 2013). Sea tidal flats, known as getbol in Korea, are broad, low-gradient, coastal muddy marshes, and play important roles in the nutrient recycling and restoration of contaminated coastal ecosystems (Winberg et al., 2007; Math et al., 2012). "
    [Show abstract] [Hide abstract]
    ABSTRACT: A Gram-staining-negative, strictly aerobic bacterial strain motile by gliding, designated GY20T, was isolated from a tidal flat at Gwangyang bay, South Korea. Cells were moderately halophilic, catalase- and oxidase-positive rods. Growth of strain GY20T was observed at 10-30 °C (optimum, 25 °C), at pH 6.5-11.0 (optimum, pH 7.0-8.0) and in the presence of 1-5 % (w/v) NaCl (optimum, 2-3 %). Only MK-6 was detected as the isoprenoid quinone and iso-C15:0, iso-C15:1 G, summed feature 3 (comprising C16:1 ω7c and/or C16:1 ω6c), summed feature 9 (comprising iso-C17:1 ω9c and/or C16:0 10-methyl), iso-C17:0 3-OH and iso-C15:0 3-OH as major fatty acids. Strain GY20T contained phosphatidylethanolamine, one unidentified glycolipid and one unidentified lipid as major polar lipids. The G+C content of the genomic DNA was 39.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain GY20T formed a tight phyletic lineage with members of the genus Maribacter. Strain GY20T was most closely related to Maribacter dokdonensis DSW-8T with sequence similarity of 96.2 %. On the basis of phenotypic, chemotaxonomic and molecular properties, strain GY20T was shown to represent a novel species within the genus Maribacter, for which the name Maribacter aestuarii sp. nov. is proposed. The type strain is GY20T (=KACC 16440T =JCM 18631T). Emended description of the genus Maribacter is also proposed.
    International Journal of Systematic and Evolutionary Microbiology 03/2013; 63(Pt 9). DOI:10.1099/ijs.0.050054-0 · 2.51 Impact Factor
  • Source
    • "nov. Bacteroidetes Antarctic sea ice Bowman et al., 2003 Brumimicrobium glaciale Bacteroidetes Antarctic sea ice Bowman et al., 2003 Maribacter arcticus Bacteroidetes Arctic marine sediment Cho et al., 2008 Maribacter antarcticus Bacteroidetes Antarctica, Southern ocean Zhang et al., 2009 Octadecabacter arcticus gen. nov. "

Show more

Similar Publications