Streptomyces axinellae sp. nov., isolated from the Mediterranean sponge Axinella polypoides (Porifera).
ABSTRACT An actinomycete strain, isolated from the marine sponge Axinella polypoides collected from Banyuls-sur-Mer, France, was characterized using a polyphasic approach. Based on its chemotaxonomic and morphological characteristics, strain Pol001(T) belongs to the genus Streptomyces. The strain is characterized by ll-diaminopimelic acid in the cell wall, menaquinones MK-9(H(4), H(6), H(8)) and a DNA G+C content of 71.0 mol%. It forms a separate phyletic line based on phylogenetic analyses of the nearly complete 16S rRNA gene sequence. Strain Pol001(T) could be differentiated from other closely related Streptomyces species with validly published names by phenotypic and genotypic analysis. DNA-DNA hybridization between strain Pol001(T) and closely related reference strains further confirmed that strain Pol001(T) represents a novel taxon of the genus Streptomyces. Therefore, it is proposed that strain Pol001(T) represents a novel species in the genus Streptomyces, Streptomyces axinellae sp. nov.; the type strain is Pol001(T) (=DSM 41948(T) =CIP 109838(T)).
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ABSTRACT: Actinobacteria are ubiquitous in the marine environment, playing an important ecological role in the recycling of refractory biomaterials and producing novel natural products with pharmic applications. Actinobacteria have been detected or isolated from the marine creatures such as sponges, corals, mollusks, ascidians, seaweeds, and seagrass. Marine organism-associated actinobacterial 16S rRNA gene sequences, i.e., 3,003 sequences, deposited in the NCBI database clearly revealed enormous numbers of actinobacteria associated with marine organisms. For example, RDP classification of these sequences showed that 112 and 62 actinobacterial genera were associated with the sponges and corals, respectively. In most cases, it is expected that these actinobacteria protect the host against pathogens by producing bioactive compounds. Natural products investigation and functional gene screening of the actinobacteria associated with the marine organisms revealed that they can synthesize numerous natural products including polyketides, isoprenoids, phenazines, peptides, indolocarbazoles, sterols, and others. These compounds showed anticancer, antimicrobial, antiparasitic, neurological, antioxidant, and anti-HIV activities. Therefore, marine organism-associated actinobacteria represent an important resource for marine drugs. It is an upcoming field of research to search for novel actinobacteria and pharmaceutical natural products from actinobacteria associated with the marine organisms. In this review, we attempt to summarize the present knowledge on the diversity and natural products production of actinobacteria associated with the marine organisms, based on the publications from 1991 to 2013.Applied microbiology and biotechnology. 07/2014;
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ABSTRACT: Two actinomycete strains, KK1-2T and CPB4-7, were isolated from marine sediments collected in Khai Island, Chumphon province, Thailand. Their taxonomic positions were determined using polyphasic approach. The morphological, cultural and chemotaxonomic characteristics of these isolates were consistent with the classification of the genus Streptomyces. They contained LL-diaminopimelic acid in their cell wall peptidoglycan; the whole-cell sugars were ribose and glucose. The predominant menaquinones were MK9-(H6) and MK9-(H8). The polar lipids were phosphatidylethanolamine (PE), phosphatidylinositol (PI), diphosphatidylglycerol (DPG), phosphatidylglycerol (PG) and phosphatidylinositol mannosides (PIMs). The predominant cellular fatty acids were anteiso-C15:0, iso-C16:0 and iso-C15:0. On the basis of 16S rRNA gene sequence similarity studies, these isolates were closely related to Streptomyces xinghaiensis JCM 16958T (98.2%), Streptomyces rimosus subsp. paromomycinus JCM 4541T (98.1%), Streptomyces sclerotialus JCM 4828T (98.1%) and Streptomyces flocculus JCM 4476T (98.0%). The G+C content of the genomic DNA of strain KK1-2T and CPB4-7 were 73.3 and 74.2 mol%, respectively. They could be clearly distinguished from these type strains by a low DNA-DNA relatedness and phenotypic differences. On the basis of these results, these strains represent a novel species of the genus Streptomyces, for which the name Streptomyces chumphonensis sp. nov. (type strain KK1-2T = JCM 18522T = TISTR 2016T = PCU 330T) is proposed.INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 05/2014; · 2.11 Impact Factor
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ABSTRACT: Covering: up to December 2012Actinomycetes are known for their unprecedented ability to produce novel lead compounds of clinical and pharmaceutical importance. This review focuses on the diversity, abundance and methodological approaches targeting marine sponge-associated actinomycetes. Additionally, novel qPCR data on actinomycete abundances in different sponge species and other environmental sources are presented. The natural products literature is covered, and we are here reporting on their chemical structures, their biological activities, as well as the source organisms from which they were isolated.Natural Product Reports 02/2014; · 10.18 Impact Factor