Isolation of Candida dubliniensis from denture wearers

Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Bauru, SP, Brazil.
Journal of Medical Microbiology (Impact Factor: 2.25). 08/2009; 58(Pt 7):959-62. DOI: 10.1099/jmm.0.008391-0
Source: PubMed


Candida albicans is considered the most important Candida species able to cause oral infections in denture wearers. In recent years, Candida dubliniensis has emerged as a pathogenic yeast in humans. The close phenotypic similarities of C. albicans and C. dubliniensis have led to the misidentification of these species. In this work, our aim was to verify through PCR the presence of C. dubliniensis in palate and maxillary denture samples from 112 denture wearers presenting with or without denture-related stomatitis (DRS). C. dubliniensis was isolated at low rates from both palate (5.3 % and 10.7 %) and maxillary denture (5.3 % and 8.9 %) samples from wearers regardless of the presence of the disease. However, when C. dubliniensis was detected in individuals with DRS, it was always associated with C. albicans. In addition, our results showed that C. albicans was the most commonly identified candidal species in maxillary denture and hard palate samples from DRS patients (78.5 % and 89.2 %, respectively) as well as from controls (31.2 % and 28.5 %, respectively). In conclusion, C. dubliniensis was detected in the oral environment of denture wearers. The association of C. dubliniensis with C. albicans occurred in approximately 10 % of the DRS cases.

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    • "Whilst some phenotypic tests generated ambiguous results, 'gold-standard' DNA analysis by PCR amplification methods based on differences in the sequences of HWP1 and topoisomerase II genes, and sequence analysis of the D1/D2 domain of the large subunit rRNA gene, permitted the definitive identification of the nine isolates as Candida dubliniensis. Other authors have previously reported the specificity and suitability of these methods to identify clinical isolates of C. dubliniensis (Bosco- Borgeat et al., 2011; Gasparoto et al., 2009; Khlif et al., 2009; Marcos-Arias et al., 2009; Sahand et al., 2009). "
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    ABSTRACT: Candida dubliniensis is a pathogenic yeast of the genus Candida closely related to Candida albicans. The phenotypic similarity of these two species often leads to misidentification of C. dubliniensis isolates in clinical samples. DNA-based methods continue to be the most effective means of discriminating accurately between the two species. Here we report on the identification of nine unusual Candida isolates that showed ambiguous identification patterns on the basis of their phenotypic and immunological traits. The isolates were categorized into two groups. Group I isolates were unable to produce germ tubes and chlamydospores and to agglutinate commercial latex particles coated with a monoclonal antibody highly specific for C. dubliniensis. Group II isolates grew as pink and white colonies on CHROMagarTM Candida and ChromIDTM Candida respectively. Carbohydrate assimilation profiles obtained with API ID 32C® together with PCR amplification with specific primers and DNA sequencing allowed a reliable identification of the nine unusual clinical isolates as Candida dubliniensis. Copyright © 2014, the Society for General Microbiology.
    Journal of Medical Microbiology 12/2014; 64(Pt_2). DOI:10.1099/jmm.0.078832-0 · 2.25 Impact Factor
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    • "DS is an inflammatory lesion of the palatal mucosa under complete or partial removable dentures and affects up to 65% of denture wearers. There are various factors that influence the onset and severity of DS: denture trauma, continuous denture wearing, salivary flow, denture cleanliness , denture base material, denture age, cellular immunity, smoking, dietary factors, pH of the denture plaque and oral microbiota (Coco et al., 2008; Gasparoto et al., 2009). "
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    ABSTRACT: The aim of this study was to isolate, quantify, identify, and compare opportunistic microorganisms (Candida and Staphylococcus genera and Enterobacteriaceae/Pseudomonadaceae families) from prosthesis-fitting surfaces, the hard palate, and mouth rinses of individuals wearing removable maxillary prosthesis with (50) and without (50) lesions of denture stomatitis (DS). The strains were collected and identified using phenotypic, biochemical and molecular tests. The counts of microorganisms were significantly higher in the group of individuals with DS (P < 0.05). C. albicans was the most frequently isolated yeast species in both groups, following by C. tropicalis and C. glabrata. Six isolates were identified as C. dubliniensis. S. aureus and S. epidermidis were the most frequent Staphylococcus species in both groups. Klebsiella pneumoniae was the predominant species in both groups. The association between Candida spp. and bacteria isolated in this study with DS suggests that these microorganisms may play important roles in the establishment and persistence of this disease.
    Diagnostic microbiology and infectious disease 06/2013; 76(4). DOI:10.1016/j.diagmicrobio.2013.05.001 · 2.46 Impact Factor
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    ABSTRACT: Patients using obturator prostheses often present denture-induced stomatitis. In order to detect the presence of oral Candida albicans in patients with oronasal communications and to evaluate the effectiveness of a topical antifungal treatment, cytological smears obtained from the buccal and palatal mucosa of 10 adult patients, and from the nasal acrylic surface of their obturator prostheses were examined. A therapeutic protocol comprising the use of oral nystatin (Mycostatin) and prosthesis disinfection with sodium hypochlorite was prescribed for all patients. Seven patients were positive for C. albicans in the mucosa, with 1 negative result for the prosthetic surface in this group of patients. Post-treatment evaluation revealed the absence of C. albicans on prosthesis surface and on the oral mucosa of all patients. The severity of the candidal infection was significantly higher in the palatal mucosa than in the buccal mucosa, but similar in the palatal mucosa and prosthesis surface, indicating that the mucosa underlying the prosthesis is more susceptible to infection. The therapeutic protocol was effective in all cases, which emphasizes the need for denture disinfection in order to avoid reinfection of the mucosa.
    Brazilian dental journal 01/2009; 20(4):336-40. DOI:10.1590/S0103-64402009000400013
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