Gradient lithography of engineered proteins to fabricate 2D and 3D cell culture microenvironments

NSF Nanoscale Science and Engineering Center-NSEC, University of California, 3112 Etcheverry Hall, Berkeley, CA 94720-1740, USA.
Biomedical Microdevices (Impact Factor: 2.88). 07/2009; 11(5):1127-34. DOI: 10.1007/s10544-009-9329-1
Source: PubMed


Spatial patterning of proteins is a valuable technique for many biological applications and is the prevailing tool for defining microenvironments for cells in culture, a required procedure in developmental biology and tissue engineering research. However, it is still challenging to achieve protein patterns that closely mimic native microenvironments, such as gradient protein distributions with desirable mechanical properties. By combining projection dynamic mask lithography and protein engineering with non-canonical photosensitive amino acids, we demonstrate a simple, scalable strategy to fabricate any user-defined 2D or 3D stable gradient pattern with complex geometries from an artificial extracellular matrix (aECM) protein. We show that the elastic modulus and chemical nature of the gradient profile are biocompatible and allow useful applications in cell biological research.

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Available from: Xiang Zhang, Oct 07, 2015
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    • "Scaffolds formed from these materials have inherent bioactivity and could aid the reconstitution of natural cell-matrix interactions. Wang and colleagues demonstrated that scaffolds could be produced via STL from engineered peptides containing photosensitive non-canonical amino acids [100]. This approach has the additional advantage of allowing the interactions of the scaffolds with cells to be controlled and offers another route to the patterning of cell function in constructs. "
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