Silver nanoparticles inhibit VEGF induced cell proliferation and migration in bovine retinal endothelial cells
ABSTRACT Angiogenesis, the growth of new blood vessels from pre-existing vasculature is of physiological and pathological importance. We have investigated the anti-angiogenic potential of silver nanoparticles, produced by Bacillus licheniformis. Bovine retinal endothelial cells (BRECs) were treated with the different concentrations of silver nanoparticles for 24 h in the presence and absence of vascular endothelial growth factor (VEGF), where 500 nM (IC50) of silver nanoparticle concentration, was able to block the proliferation and migration of BRECs. The cells showed a clear enhancement in caspase-3 activity and formation of DNA ladders, evidence of induction of apoptosis. Here we report for the first time that silver nanoparticles inhibit cell survival via PI3K/Akt dependent pathway in Bovine retinal endothelial cells.
- SourceAvailable from: Sawsan Mohammed EL-sonbaty[Show abstract] [Hide abstract]
ABSTRACT: Silver nanoparticles (AgNPs) were biologically synthesized using aqueous extract of Agaricus bisporus fungi. Physicochemical analysis of silver nanoparticles revealed that they are of spherical shape ranged size of 8–20 nm, and their zeta potential equal −7.23 mV. Silver nanoparticles showed a dose-dependent cytotoxic effect on MCF-7 breast cancer cells with LD50 (50 μg/ml). Mice bearing Ehrlich solid tumor treated with AgNPs and exposed to gamma radiation significantly ameliorated superoxide dismutase and catalase activity and reduced glutathione with an increase in malondialdehyde and nitric oxide levels compared to tumor group. Gamma radiation with AgNPs induced apoptotic cell count in Ehrlich solid tumor cells from 68.3 (treated with AgNPs) to 98.1 % (treated with AgNPs with gamma radiation) via a mechanism involved caspase-3. Histological sections of tumor tissue of mice treated with AgNPs showed antiangiogenesis effect of AgNPs. The overall result indicates that AgNPs synergize with gamma radiation, promising a potential combined therapy of cancer.08/2013; 4(4-5). DOI:10.1007/s12645-013-0038-3
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ABSTRACT: Silver nanoparticles (AgNPs) have been used as an antimicrobial and disinfectant agents. However, there is limited information about antitumor potential. Therefore, this study focused on determining cytotoxic effects of AgNPs on MDA-MB-231 breast cancer cells and its mechanism of cell death. Herein, we developed a green method for synthesis of AgNPs using culture supernatant of Bacillus funiculus, and synthesized AgNPs were characterized by various analytical techniques such as UV-visible spectrophotometer, particle size analyzer, and transmission electron microscopy (TEM). The toxicity was evaluated using cell viability, metabolic activity, and oxidative stress. MDA-MB-231 breast cancer cells were treated with various concentrations of AgNPs (5 to 25 μ g/mL) for 24 h. We found that AgNPs inhibited the growth in a dose-dependent manner using MTT assay. AgNPs showed dose-dependent cytotoxicity against MDA-MB-231 cells through activation of the lactate dehydrogenase (LDH), caspase-3, reactive oxygen species (ROS) generation, eventually leading to induction of apoptosis which was further confirmed through resulting nuclear fragmentation. The present results showed that AgNPs might be a potential alternative agent for human breast cancer therapy.07/2013; 2013:535796. DOI:10.1155/2013/535796
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ABSTRACT: Abstract Silver nanoparticles (AgNPs) have many biological applications in biomedicine, biotechnology and other life sciences. Depending on the size, shape and the type of a carrier, AgNPs demonstrate different physical and chemical properties. AgNPs have strong antimicrobial, antiviral and antifungal activity, thus they are used extensively in a range of medical settings especially in wound dressings but also cosmetics.This study was undertaken to examine the potential toxic effects of 15 nm polyvinylpyrrolidone-coated AgNPs on Normal Human Primary Keratinocytes (NHEK). Cells were treated with different concentrations of AgNPs and then cell viability, metabolic activity and other biological and biochemical aspects of keratinocytes functioning were studied. We observed that AgNPs decrease keratinocyte viability, metabolism and also proliferatory and migratory potential of these cells. Moreover, longer exposure resulted in activation of caspase 3/7 and DNA damage. Our studies show for the first time, that in primary keratinocytes AgNPs may present possible danger, concerning activation of genotoxic and cytotoxic processes depending on concentration.Biological Chemistry 09/2012; 394(1). DOI:10.1515/hsz-2012-0202 · 2.69 Impact Factor