Verniz vitral incolor 500®: a mounting medium alternative and economically viable

Acta Botanica Brasilica (Impact Factor: 0.55). 06/2006; 20(2):257-264. DOI: 10.1590/S0102-33062006000200002


There are different imported mounting mediuns commercialized at a relative high price for producing permanent slides of stained plant sections. These mounting mediuns become harder as they dry and preserve the good conditions of the plant material for indetermined time. The most used mouting mediuns to plant anatomy are: Canada balsam, Euparal®, Entellan® and Permount®. Aiming to reduce costs of producing permanent plant anatomy slides, alternative mediuns produced by brazilian industries, used mostly in artesanal work, were tested. Among the different synthetic mediuns tested, the verniz vitral incolor 500® showed properties compatible to be used as an efficient mounting medium, in substitution to the imported synthetic resins, at a lower cost and without alterations in the routine process. The verniz vitral incolor 500® allowed the production of permanent slides with plant organs sectioned hand free as well in rotatory microtome, after paraffin or historesin embedding, keeping unaltered the tissue and color charactheristics. The results showed that the tradicional resins can be replaced by the verniz vitral incolor 500®, not compromising the quality of the slides.

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Available from: Suzane Margaret Fank-de-Carvalho, Aug 13, 2014
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    • "Transversal cuttings of 10 µm or less and paradermal cuttings of 3 to 5 µm were obtained in microtome , adhered to laminas and stained either by aqueous saphranin and/or toluidine blue or fuchsin and Astra blue (Kraus and Arduin 1997). The excess staining was removed by the immersion of the laminas in tap water and, after drying at room temperature, they were mounted using acrylic resin Acrilex® (Fank-de-Carvalho and Graciano-Ribeiro 2005, Paiva et al. 2006). The length, greater width and weight of two leaves of 10 masculine and 10 feminine plants (totalling 40 leaves) were measured. "

    Acta Botanica Hungarica 03/2009; 51(1):115-127. DOI:10.1556/ABot.51.2009.1-2.14
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    • "The free-hand sections were cleared in 50% sodium hypochlorite solution (Kraus and Arduin, 1997), stained with 1% safranin-alcian blue (Luque et al., 1996), dehydrated in an ethanol series and butyl acetate, and mounted in synthetic resin (Paiva et al., 2006). Photomicrographs were taken using a Zeiss Axioskop microscope, and the images were captured with the Motion Image Plus 2.0 software. "
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    ABSTRACT: We examined the stem anatomy of Manihot esculenta Crantz and its hybrid with M. oligantha Pax. Cross-sections were stained with safranin-alcian blue. Manihot esculenta and its hybrid were found to have the same vascular structure in bicollateral bundles with internal phloem, which has been associated with drought resistance in other plant groups. If this association is established for cassava, it would facilitate the selection of lines that are more adapted to arid regions. This is the first report of internal phloem in this genus.
    Genetics and molecular research: GMR 01/2009; 8(3):1139-46. DOI:10.4238/vol8-3gmr629 · 0.78 Impact Factor
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    ABSTRACT: This study describes the stem anatomy of Manihot esculenta Crantz and its hybrid with M. oligantha Pax. This is the first report of the presence of internal phloem in this genus. Cross-sections were made, fixed, conserved and colored by safranin-alcian blue. M. esculenta and its hybrid showed the same vascular structure in bicollateral bundles with internal phloem, which may be related to drought resistance and enables selection of individuals that are more adapted to arid regions.
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