Verniz vitral incolor 500®: a mounting medium alternative and economically viable

Acta Botanica Brasilica (Impact Factor: 0.37). 06/2006; 20(2):257-264. DOI: 10.1590/S0102-33062006000200002

ABSTRACT There are different imported mounting mediuns commercialized at a relative high price for producing permanent slides of stained plant sections. These mounting mediuns become harder as they dry and preserve the good conditions of the plant material for indetermined time. The most used mouting mediuns to plant anatomy are: Canada balsam, Euparal®, Entellan® and Permount®. Aiming to reduce costs of producing permanent plant anatomy slides, alternative mediuns produced by brazilian industries, used mostly in artesanal work, were tested. Among the different synthetic mediuns tested, the verniz vitral incolor 500® showed properties compatible to be used as an efficient mounting medium, in substitution to the imported synthetic resins, at a lower cost and without alterations in the routine process. The verniz vitral incolor 500® allowed the production of permanent slides with plant organs sectioned hand free as well in rotatory microtome, after paraffin or historesin embedding, keeping unaltered the tissue and color charactheristics. The results showed that the tradicional resins can be replaced by the verniz vitral incolor 500®, not compromising the quality of the slides.

  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: The "aroeira" (Myracrodruon urundeuva Allemão) is a tropical tree with limited geographic distribution in South America, being found in drier formations such as the Cerrado and Caatinga. Empirically it is used with antiseptic, antiinflammatory, antiulcer, antidiarrhoeal and others. In this study we used mature leaves and expanded from the third and fourth nodes. Studies venation and morphology, anatomy and histochemistry were performed by the usual laboratory plant anatomy or the usual techniques of plant anatomy. For histochemical study of the fresh cuts various reagents and specific stains were used. The blade is elliptical leaflets with acute apex, oblique base, obtuse angle, entire margin and slightly wavy. Shows the pattern of venation feather- veined, pinnate type based generally asymmetrical and oblique. The indumentum is sericeous with trichomes deciduous. The consistency of the lamina is papyracea. The cuticle of leaflets, is thinner on the lower epidermis than on the upper epidermis, palisade parenchyma with a cell layer and spongy parenchyma with three cell layers with idioblasts containing crystals of CaCO3, tector trichomes simple multicellular with two or more cells are observed on both sides of the leaflets. The histochemical analysis revealed the presence of starch granules, crystals of calcium oxalate, fatty compounds, resins, phenolics and alkaloids compounds. The structural data obtained in this study may assist in ecophysiological characterization of the species and provide evidence for the identification of herbal medicines produced from that plant organ
    Boletín Latinoamericano y del Caribe de Plantas Medicinales y Aromáticas. 01/2011; 10.
  • [Show abstract] [Hide abstract]
    ABSTRACT: The developmental anatomy of the stems of Marcetia taxifolia (A. St.-Hil.) DC. fits the patterns described for other Melastomataceae. The galling effect of the Lepidoptera caused discrete structural alterations and conspicuous histochemical profiles. Epidermis and cortical parenchyma were hyperplasic with hypertrophied cells. The vascular system showed smaller changes. Tracheal elements did not change in width, refuting the constriction hypothesis, i.e., no improvement in water supply occurs in this gall system. Fiber lignification increased, providing additional support for the gall structure. A true nutritive tissue was redifferentiated from pith cells and accumulated two groups of metabolites. The first, consisting of starch, reducing sugars, and polyphenols, was detected in the outer cell layers, and the second, consisting of lipids and terpenoids, was detected in the inner ones. Histochemical analysis revealed that the distribution of these compounds formed gradients, with the first group being more concentrated outwards, and the second being more concentrated inwards. These gradients differ from those described for other insect galls and seem to be specific for lepidopteran galls. This is the first description of such a gradient in Lepidoptera-induced galls and shows that the current view that these galls are simple and nonnutritive should be changed. Résumé : Le développement anatomique des tiges du Marcetia taxifolia (A. St.-Hil.) DC. correspond au patron décrit pour d'autres Melastomataceae. Les galles causées par les Lepidoptera induisent des altérations structurales discrètes et des profiles his-tochimiques évidents. On observe une hyperplasie de l'épiderme et du parenchyme cortical ainsi que des cellules hypertro-phiées. Il y a de moindres changements au système vasculaire. La largeur des éléments trachéaux ne change pas, réfutant ainsi l'hypothèse de la constriction, c.-à -d. aucune amélioration de l'approvisionnement en eau ne survient au niveau du système de la galle. La lignification des fibres augmente, ajoutant au support de la structure de la galle. Un véritable tissu nutritif se redifférencie à partir des cellules de la moelle et accumule deux groupes de métabolites. Le premier comporte de l'amidon, des sucres réducteurs et des polyphénols, se situant dans les couches cellulaires externes et le second comporte des lipides et des terpénoïdes, que l'on retrouve dans les couches internes. L'analyse histochimique révèle que la distribution de ces composés forme des gradients, le premier groupe étant plus concentré vers l'extérieur et le second plus concentré vers l'intérieur. Ces gradients diffèrent de ceux déjà décrits pour d'autres galles d'insectes et semblent spécifiques aux galles de lépidoptères. Il s'agit de la première description d'un tel gradient chez les galles des Lepidoptera, ce qui montre que le concept actuel voulant que ces galles soient simples et non nutritionnelles doit être modifié. [Traduit par la Rédaction] Mots-clés : hypertrophie cellulaire, redifférenciation cellulaire, hyperplasie, gradients métaboliques, tissu nutritif.
    Botany 11/2013; 91(11):752-760. · 1.23 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: A revision of Pusillanthus Kuijt for Brazil is presented. The recently described monotypic genus occurs in Venezuela, Guyana, and Brazil. In Brazil, it is distributed only within the Caatinga domain, in the states of Alagoas, Bahia and Paraíba. The new combination Pusillanthus pubescens (Rizzini) Caires is proposed and the species Phthirusa caatingae Rizzini, Pusillanthus trichodes (Rizzini) Kuijt, and Struthanthus pubescens var. bahiensis Rizzini are treated as its taxonomic synonyms. An illustrative plate and a distribution map for the genus in Brazil are presented, as well as information about leaf architecture, and stem, leaf, and pollen grain surfaces (based on scanning electron microscopy).
    Acta Botanica Brasilica 09/2012; 26(3):668-674. · 0.37 Impact Factor

Full-text (3 Sources)

Available from
Aug 13, 2014