Antibiotic Resistance of Gram Negatives isolates from loggerhead sea turtles (Caretta caretta) in the central Mediterranean Sea.

Dipartimento di Sanità Pubblica Veterinaria, Università degli Studi di Messina, Polo Universitario SS Annunziata, 98167 Messina, Italy.
Marine pollution bulletin (Impact Factor: 2.63). 06/2009; 58(9):1363-6. DOI: 10.1016/j.marpolbul.2009.04.020
Source: PubMed

ABSTRACT Previous studies on fish and marine mammals support the hypothesis that marine species harbor antibiotic resistance and therefore may serve as reservoirs for antibiotic-resistance genetic determinants. The aim of this study was to assess the resistance to antimicrobial agents of Gram negative strains isolated from loggerhead sea turtles (Carettacaretta). Oral and cloacal swabs from 19 live-stranded loggerhead sea turtles, with hooks fixed into the gut, were analyzed. The antimicrobial resistance of the isolates to 31 antibiotics was assessed using the disk-diffusion method. Conventional biochemical tests identified Citrobacter spp., Proteus spp., Enterobacter spp., Escherichia spp., Providencia spp., Morganella spp., Pantoea spp., Pseudomonas spp. and Shewanella spp. Highest prevalences of resistance was detected to carbenicillin (100%), cephalothin (92.6%), oxytetracycline (81.3%) and amoxicillin (77.8%). The isolates showing resistance to the widest range of antibiotics were identified as Citrobacterfreundii, Proteusvulgaris, Providenciarettgeri and Pseudomonasaeruginosa. In this study, antibiotic resistant bacteria reflect marine contamination by polluted effluents and C.caretta is considered a bioindicator which can be used as a monitor for pollution.

1 Bookmark
  • [Show abstract] [Hide abstract]
    ABSTRACT: Anthropogenic debris in the environment affects many species that accidentally ingest it. The aim of this study is to evaluate the quantity and composition of marine litter ingested by loggerheads in Sardinia, thus supplying for the lack of data in the existing literature for this area. Seventeen of the 121 (14.04%) monitored turtles presented debris in their digestive tracts. Litter in faecal pellet of alive individuals (n=91) and in gastro-intestinal contents of dead ones (n=30) was categorized, counted and weighed. User plastic was the main category of ingested debris with a frequency of occurrence of 13.22% of the total sample, while sheet (12.39%) and fragments (9.09%) were the most relevant sub-categories. This study highlights for the first time the incidence of litter in alive turtles in Sardinia. This contribution improves the knowledge about marine litter interaction on Caretta caretta as bio-indicator. Results will be useful for the Marine Strategy implementation.
    Marine environmental research 01/2013; · 2.34 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Studies on marine mammals can inform our understanding of the environmental health of the ocean. To evaluate the potential for changes in antimicrobial resistance, we analyzed a database spanning 2004-2010 that consisted of bacterial isolate identity and antimicrobial sensitivity for stranded pinnipeds in the Northwest Atlantic. Samples (n = 170) from treated animals yielded 310 bacterial isolates representing 24 taxa. We evaluated changes in antimicrobial class resistance from 2004 to 2010 for eight taxa. Escherichia coli displayed a significant increase in resistance to several antimicrobial classes. Other taxa displayed significant increases in resistance to aminoglycosides, and/or fluoroquinolones. In addition, we observed a significant increase in multiple antimicrobial resistance in cultures from untreated animals. These results demonstrate an increase in resistance among common bacterial pathogens of marine mammals over a time span of 6 years.
    EcoHealth 05/2013; · 2.20 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Bleeding in acute promyelocytic leukemia (APL) is mainly associated with disseminated intravascular coagulation and hyperfibrinolysis. Annexin II (Ann II) is a co-receptor for plasminogen and tissue plasminogen activator (tPA). This study demonstrates that abnormally high levels of Ann II expression in APL cells increase the production of plasmin. Hyperfibrinolysis could be corrected by all-trans retinoic acid (ATRA) or ATRA plus arsenic trioxide therapy in patients with APL. SiRNA targetting Ann II caused a decrease in tPA-dependent plasmin generation, while Ann II cDNA transfectant stimulated plasmin production. These results suggest that Ann II play an important role in hyperfibrinolysis in APL.
    Leukemia research 12/2010; 35(7):879-84. · 2.36 Impact Factor

Full-text (3 Sources)

Available from
May 16, 2014