Article

Molecular characterization of a human cation-Cl- cotransporter (SLC12A8A, CCC9A) that promotes polyamine and amino acid transport.

Department of Medicine, Nephrology Research Group of the CHUQ-L'Hôtel-Dieu de Québec Institution, Laval University, Québec G1R2J6, Canada.
Journal of Cellular Physiology (impact factor: 3.87). 06/2009; 220(3):680-9. DOI:10.1002/jcp.21814
Source: PubMed

ABSTRACT Cation-Cl- cotransporters (CCCs) belong to a large family of proteins that includes 9 isoforms, two of which have still not been ascribed a transport function (CCC8 and CCC9) while the others are all known to promote Cl(-)-coupled Na+ and/or K+ movement at the cell surface. The CCCs are also included in a larger family termed amino acid-polyamine-organocation carriers (APCs). In contrast to the CCCs, however, polyamine (PA) transporters have thus far been isolated from unicellular species exclusively and do not all belong to the APC family. In this work, we have found that a splice variant of CCC9 (CCC9a) promotes PA-amino acid transport at the surface of HEK-293 cells. We have also found that the influx of PAs in CCC9a-expressing cells is inhibited by pentamidine as well as furosemide, and that it increases further in the presence of specific amino acids but not of Na+, K+, or Cl-. Hence, a group of substrates that are directly transported by CCC9 and the molecular identity of a PA transport system in animal cells may have been uncovered for the first time. These findings are of special interest given that intracellular PAs play a key role in cell proliferation.

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Keywords

amino acid-polyamine-organocation carriers
 
animal cells
 
Cation-Cl- cotransporters
 
CCC9a
 
CCC9a-expressing cells
 
cell proliferation
 
cell surface
 
Cl(-)-coupled Na+
 
Cl-
 
HEK-293 cells
 
includes 9 isoforms
 
intracellular PAs
 
large family
 
larger family
 
molecular identity
 
PA transport system
 
specific amino acids
 
splice variant
 
transport function
 
unicellular species
 

Nikolas D Daigle