Article

Isolation and characterization of surfactin produced by Bacillus polyfermenticus KJS-2.

Department of Smart Food and Drugs, Inje University, Gimhae, 621-749, Korea.
Archives of Pharmacal Research (impact factor: 1.59). 06/2009; 32(5):711-5. DOI:10.1007/s12272-009-1509-2 pp.711-5
Source: PubMed

ABSTRACT Bacillus polyfermenticus KJS-2 (BP-KJS-2) was used to produce a lipopeptide-type surfactin. To accomplish this, a surfactin-producing BP-KJS-2 was fermented by soybeans. The surfactin was then purified by a procedure including ethanol treatment and preparative chromatography. Next, the biochemical structure of the purified surfactin was analyzed by electrospray ionization mass spectrometry (ESI-MS) and high-resolution ESI Q-Tof mass spectrometry (Q-Tof MS). In addition, the masses of the four peaks were determined to be 1007, 1021, 1035, and 1049 m/z revealing that the compound was mixture with quasi-molecular ions. Taken together, these findings indicated that the lipopeptide had a cyclic structure and amino acid composition of Gln-Leu-Leu-Leu-Val-Asp-Leu-Leu, and that the major lipopeptide product of BP-KJS-2 is the surfactin isoform. In addition, this lipopeptide showed strong antimicrobial activity against bacteria at the level of 0.05 mg/mL.

0 0
 · 
0 Bookmarks
 · 
38 Views
  • Source
    Article: Surfactin–A Review on Biosynthesis, Fermentation, Purification and Applications
    Nikhil S Shaligram, Rekha S Singhal
    [show abstract] [hide abstract]
    ABSTRACT: Surfactin, a bacterial cyclic lipopeptide, is produced by various strains of Bacillus subti-lis and is primarily recognized as one of the most effective biosurfactants. It has the ability to reduce surface tension of water from 72 to 27 mN/m at a concentration as low as 0.005 %. The structure of surfactin consists of seven amino acids bonded to the carboxyl and hydrox-yl groups of a 14-carbon fatty acid. Surfactin possesses a number of biological activities such as the ability to lyse erythrocytes, inhibit clot formation, lyse bacterial spheroplasts and protoplasts, and inhibit cyclic 3 ' ,5-monophosphate diesterase. The high cost of produc-tion and low yields have limited its use in various commercial applications. Both sub-merged and solid-state fermentation have been investigated with the mutational approach to improve the productivity. In this review, current state of knowledge on biosynthesis of surfactin, its fermentative production, purification, analytical methods and biomedical appli-cations is presented.
Data provided are for informational purposes only. Although carefully collected, accuracy cannot be guaranteed. The impact factor represents a rough estimation of the journal's impact factor and does not reflect the actual current impact factor. Publisher conditions are provided by RoMEO. Differing provisions from the publisher's actual policy or licence agreement may be applicable.

Keywords

amino acid composition
 
Bacillus polyfermenticus KJS-2
 
biochemical structure
 
BP-KJS-2
 
cyclic structure
 
electrospray ionization mass spectrometry
 
ethanol treatment
 
four peaks
 
high-resolution ESI Q-Tof mass spectrometry
 
lipopeptide
 
lipopeptide-type surfactin
 
major lipopeptide product
 
preparative chromatography
 
purified surfactin
 
Q-Tof MS
 
quasi-molecular ions
 
strong antimicrobial activity
 
surfactin
 
surfactin isoform
 
surfactin-producing BP-KJS-2