Yoshimoto, T., Yasuda, K., Tanaka, H., Nakahira, M., Imai, Y., Fujimori, Y. et al. Basophils contribute to T(H)2-IgE responses in vivo via IL-4 production and presentation of peptide-MHC class II complexes to CD4+ T cells. Nat. Immunol. 10, 706-712

Department of Immunology and Medical Zoology, Hyogo College of Medicine, Nishinomiya, Hyogo, Japan.
Nature Immunology (Impact Factor: 20). 08/2009; 10(7):706-12. DOI: 10.1038/ni.1737
Source: PubMed

ABSTRACT Basophils express major histocompatibility complex class II, CD80 and CD86 and produce interleukin 4 (IL-4) in various conditions. Here we show that when incubated with IL-3 and antigen or complexes of antigen and immunoglobulin E (IgE), basophils internalized, processed and presented antigen as complexes of peptide and major histocompatibility complex class II and produced IL-4. Intravenous administration of ovalbumin-pulsed basophils into naive mice 'preferentially' induced the development of naive ovalbumin-specific CD4+ T cells into T helper type 2 (T(H)2) cells. Mice immunized in this way, when challenged by intravenous administration of ovalbumin, promptly produced ovalbumin-specific IgG1 and IgE. Finally, intravenous administration of IgE complexes rapidly induced T(H)2 cells only in the presence of endogenous basophils, which suggests that basophils are potent antigen-presenting cells that 'preferentially' augment T(H)2-IgE responses by capturing IgE complex.

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Available from: Yoshihiro Fujimori, Mar 11, 2014
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    • "Basophils are IgE-activated granulocytes that, unlike tissue-resident mast cells, circulate in the blood. They play a critical role in the IgE-mediated development of chronic allergic reactions and inflammation [97] [98], and they can also promote polarization towards Th2 responses by IgE-independent antigen presentation in mice [99] [100]. Basophils are recruited to a tick-feeding site and accumulate in the host skin during second and consequent (but not primary) tick infestation, where they act as important tick rejection factors [101] [102]. "
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    ABSTRACT: Next generation sequencing and proteomics have helped to comprehensively characterize gene expression in tick salivary glands at both the transcriptome and the proteome level. Functional data are, however, lacking. Given that tick salivary secretions are critical to the success of the tick transmission lifecycle and, as a consequence, for host colonization by the pathogens they spread, we thoroughly review here the literature on the known interactions between tick saliva (or tick salivary gland extracts) and the innate and adaptive vertebrate immune system. The information is intended to serve as a reference for functional characterization of the numerous genes and proteins expressed in tick salivary glands with an ultimate goal to develop novel vector and pathogen control strategies. We overview all the known interactions of tick saliva with the vertebrate immune system. The provided information is important, given the recent developments in high-throughput transcriptomic and proteomic analysis of gene expression in tick salivary glands, since it may serve as a guideline for the functional characterization of the numerous newly-discovered genes expressed in tick salivary glands. Copyright © 2015. Published by Elsevier B.V.
    Journal of proteomics 07/2015; 128. DOI:10.1016/j.jprot.2015.07.005
    • "Likewise, GM-CSF amplifies IL-3-induced differentiation of basophils from bone marrow cells [30], suggesting a possible role as a growth factor for basophil precursors. Furthermore, basophils have been shown to express MHC-II and co-stimulatory molecules and act as potent antigen presenting cells (APCs) to stimulate TH2 type responses both in vitro [31] and in vivo [32]. It is apparent that GM-CSF has a more profound role in the modulation of immune responses beyond its role as a CSF, something that has remained under appreciated. "
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    ABSTRACT: GM-CSF was originally identified as a colony stimulating factor (CSF) because of its ability to induce granulocyte and macrophage populations from precursor cells. Multiple studies have demonstrated that GM-CSF is also an immune-modulatory cytokine, capable of affecting not only the phenotype of myeloid lineage cells, but also T-cell activation through various myeloid intermediaries. This property has been implicated in the sustenance of several autoimmune diseases like arthritis and multiple sclerosis. In contrast, several studies using animal models have shown that GM-CSF is also capable of suppressing many autoimmune diseases such as Crohn's disease, Type-1 diabetes, Myasthenia gravis and experimental autoimmune thyroiditis. Knockout mouse studies have suggested that the role of GM-CSF in maintaining granulocyte and macrophage populations in the physiological steady state is largely redundant. Instead, its immune-modulatory role plays a significant role in the development or resolution of autoimmune diseases. This is mediated either through the differentiation of precursor cells into specialized non-steady state granulocytes, macrophages and dendritic cells, or through the modulation of the phenotype of mature myeloid cells. Thus, outside of myelopoiesis, GM-CSF has a profound role in regulating the immune response and maintaining immunological tolerance. Copyright © 2015 Elsevier Ltd. All rights reserved.
    Cytokine 06/2015; 75(2). DOI:10.1016/j.cyto.2015.05.030
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    • "Moreover, we have shown that, in the gut mucosa of patients with inflammatory bowel disease, MCs co-localize with B cells at sites of inflammation (Merluzzi et al., 2010). Similar considerations can be made for basophils which were shown to express the lymph nodehoming marker CD62L (Yoshimoto et al., 2009). Interestingly, it has been reported that basophils of Lyn -/− mice, which develop a systemic lupus erythematosus-like disease, upregulate CD62L expression and home to the lymph nodes and spleen (Charles et al., 2010). "
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    ABSTRACT: It has been proven that both resting and activated mast cells (MCs) and basophils are able to induce a significant increase in proliferation and survival of naïve and activated B cells, and their differentiation into antibody-producing cells. The immunological context in which this regulation occurs is of particular interest and the idea that these innate cells induce antibody class switching and production is increasingly gaining ground. This direct role of MCs and basophils in acquired immunity requires cell to cell contact as well as soluble factors and exosomes. Here, we review our current understanding of the interaction between B cells and MCs or basophils as well as the evidence supporting B lymphocyte-MC/basophil crosstalk in pathological settings. Furthermore, we underline the obscure aspects of this interaction that could serve as important starting points for future research in the field of MC and basophil biology in the peculiar context of the connection between innate and adaptive immunity.
    Molecular Immunology 10/2014; 63(1). DOI:10.1016/j.molimm.2014.02.016
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