The relationship between muscle α-tocopherol concentration and meat oxidation in light lambs fed vitamin E supplements prior to slaughter

Journal of the Science of Food and Agriculture (Impact Factor: 1.88). 01/2015; DOI: 10.1002/jsfa.6688

ABSTRACT Background
The use of concentrates supplemented with α-tocopherol in animals is an effective method to reduce the oxidative processes that occurs in meat products. The high cost of α-tocopherol requires accurate feeding, and it is necessary to define the minimum period of α-tocopherol concentrate supplementation that will ensure an acceptable meat quality. Indoor concentrate-fed light lambs (n = 35) were supplemented with 500 mg of dl-α-tocopheryl acetate kg−1 (VE) concentrate for a period of between 4 and 28 days before slaughtering at 22–24 kg BW. Control lambs (n = 12) were not supplemented with α-tocopherol.ResultsThe α-tocopherol content in both plasma and muscles tissues increased significantly with the length of supplementation (P < 0.001). The TBARS concentration in meat decreased exponentially when the muscle α-tocopherol concentration was increased to 0.61-0.90 mg α-tocopherol kg−1 fresh meat (P < 0.05). After 7 days of display, the formation of metmyoglobin (MMb) decreased significantly as the α-tocopherol content increased to 0.31-0.60 mg α-tocopherol kg−1 of meat (P < 0.05).Conclusions
Therefore, a range of 0.61-0.90 mg of α-tocopherol kg−1 fresh meat protected fresh lamb meat from lipid oxidation and MMb formation. This level can be achieved by supplementation with 500 mg kg−1 of VE concentrate for a period of 7 to 14 days before slaughter.

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    ABSTRACT: a2 Department of Food Science and Technology, University of California, Davis, CA, USA
    Proceedings of The Nutrition Society 08/1994; 53(2):289-95. DOI:10.1079/PNS19940034 · 4.94 Impact Factor
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    ABSTRACT: Thirty wether lambs were randomly assigned to three treatments consisting of a control (C) and two vitamin E-supplemented treatments (VE), one fed 500 IU of vitamin E.lamb-1.d-1 (E500) and the other fed 1,000 IU of vitamin E.lamb-1.d-1 (E1000). After a 56-d feeding period, lambs were slaughtered and carcass traits were evaluated. Wholesale legs and loins were vacuum-packaged, stored at 4 degrees C for 7, 14, 21, or 28 d, fabricated into retail cuts, and packaged and displayed to simulate retail industry conditions. The E1000 lambs gained less (P < .05) (kg/d; total gain) and had lower (P < .05) carcass weights than the E500 lambs. Alpha-tocopherol levels in the longissimus lumborum were higher (P < .05) (5.79 vs 3.50 micrograms/g of tissue) for VE than for C; however, there was no difference in alpha-tocopherol level in longissimus lumborum between E500 and E1000. Leg retail cuts experienced greater (P < .05) lipid oxidation and received lower (P < .05) lean color scores than did loin retail cuts. Less (P < .05) lipid oxidation occurred from 1 to 7 d of display in VE retail cuts than in C retail cuts. Longer storage periods before retail display resulted in greater (P < .05) lipid oxidation at both 1 and 7 d of display and a higher (P < .05) rate of lipid oxidation during the display period. Supplementing vitamin E had the greatest effect in reducing lipid oxidation when cuts were stored for longer periods before retail display.(ABSTRACT TRUNCATED AT 250 WORDS)
    Journal of Animal Science 03/1995; 73(2):399-405. · 1.92 Impact Factor
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    ABSTRACT: To determine the relationship between plasma and tissue alpha-tocopherol concentrations during vitamin E depletion, weaned lambs were placed on a vitamin E-deficient diet for 0, 1, 2, 4, 8 and 12 weeks. alpha-Tocopherol was measured in plasma, erythrocytes, liver, adrenal, adipose tissue, three different skeletal muscles and heart muscle. The alpha-tocopherol concentration in plasma fell at the same rate as the alpha-tocopherol concentration in skeletal muscles, heart muscle, adrenal and adipose tissue. The alpha-tocopherol concentration in liver and erythrocytes fell at a faster rate than that of plasma and all muscle tissues. There were significant correlations between alpha-tocopherol concentration in plasma and alpha-tocopherol concentrations in all the tissues measured. Different skeletal muscles had significantly different concentrations of alpha-tocopherol which may relate to their differing susceptibility to nutritional myopathy. The increase in malondialdehyde in oxidatively-stressed muscle tissue and the correlation with alpha-tocopherol concentration in most muscle tissues indicated that the muscles had reduced antioxidant capacity in vitro as a result of vitamin E depletion. It was concluded that during vitamin E depletion in sheep alpha-tocopherol concentration in plasma was a good index of vitamin E status under the experimental conditions employed.
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