Article

HPLC-ESI(+)MS and MS/MS Profiling of some Herbarium-Preserved Amsinckia Species.

Journal of Agricultural and Food Chemistry (Impact Factor: 3.11). 03/2014; DOI: 10.1021/jf500425v
Source: PubMed

ABSTRACT Species of the Amsinckia genus (Boraginaceae) are known to produce potentially hepato-, pneumo- and/or geno-toxic dehydropyrrolizidine alkaloids. However, the taxonomic differentiation of Amsinckia species can be very subtle and there seems to be marked differences in toxicity towards grazing livestock. Methanol extracts of mass-limited leaf samples from herbarium specimens (collected from 1899 to 2013) of 10 Amsinckia species and one variety were analyzed using HPLC-esi(+)MS and MS/MS for the presence of potentially toxic dehydropyrrolizidine alkaloids and/or their N-oxides. Dehydropyrrolizidine alkaloids were detected in all specimens examined ranging from about one to 4000 g/g of plant. Usually occurring mainly as their N¬-oxides, the predominant alkaloids were the epimeric lycopsamine and intermedine. Also sometimes observed in higher concentrations were the 3'- and 7-acetyl derivatives of lycopsamine/intermedine and their N-oxides. Within a designated species, an inconsistent profile was often observed that may be due to natural variation, taxonomic misassignment or non-uniform degradation due to plant collection and storage differences.

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