Iannacone MR, Gheit T, Pfister H et al.Case-control study of genus-beta human papillomaviruses in plucked eyebrow hairs and cutaneous squamous cell carcinoma. Int J Cancer 134:2231-2244

Infections and Cancer Biology (ICB) Group, International Agency for Research on Cancer, 150 , Cours, Albert Thomas, 69372, Lyon CEDEX 08, France.
International Journal of Cancer (Impact Factor: 5.09). 05/2014; 134(9). DOI: 10.1002/ijc.28552
Source: PubMed


Cutaneous human papillomaviruses (HPV) have been reported in cutaneous squamous cell carcinoma (SCC). We conducted a clinic-based case-control study to investigate the association between genus-beta HPV DNA in eyebrow hairs (EBH) and SCC. EBH from 168 SCC cases and 290 controls were genotyped for genus-beta HPV DNA. SCC tumors from a subset of cases (n=142) were also genotyped. Viral load was determined in a subset of specimens positive for a single HPV type. Associations with SCC were estimated by odds ratios (OR) and 95% confidence intervals (CI) adjusted for age and sex using logistic regression. Statistical tests were two-sided. EBH DNA prevalence was greater in cases (87%) than controls (73%) (p<0.05), and the association with SCC increased with the number of HPV types present, (≥4 types vs. HPV-negative: OR=2.02, 95% CI=1.07-3.80; ptrend =0.02). Type-specific associations were observed between SCC and DNA in EBH for HPV23 (OR=1.90, 95% CI=1.10-3.30) and HPV38 (OR=1.84, 95% CI=1.04-3.24). Additionally, when compared to the controls, the DNA prevalence in EBH was significantly higher among cases for 11 of the 25 genus-beta types tested, when accounting for DNA for the same HPV type in the tumor (ORs=3.44 to 76.50). Compared to controls, the mean viral DNA load in EBH among the selected cases was greater for HPV5, HPV8, and HPV24, but lower for HPV38. SCC cases were more likely than controls to have HPV DNA+ EBH for single and multiple HPV types, providing additional support for the potential role of genus-beta HPV infections in SCC development. © 2013 Wiley Periodicals, Inc.

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Available from: Steffi Silling, Nov 19, 2014
    • "Most of the genotypes detected were of the b2 species (HPV9, HPV23, HPV37, HPV38 and HPV120), which comprises viruses with heterogeneous niches, including the oral cavity, eyebrow hairs, peri-anal tissues and skin (Bottalico et al., 2012; Schneider et al., 2013). HPV23 and HPV38 infections show a positive association with cutaneous squamous cell carcinoma (Iannacone et al., 2014). HPV8, the only member of the b1 genus detected in our samples, is a cutaneous HPV clearly involved in skin cancer development in epidermodysplasia verruciformis patients, and its early genes E2, E6 and E7 have been implicated in cell transformation in vitro (Schaper et al., 2005). "
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