Article

1H, 15N and 13C resonance assignments for the three LOTUS RNA binding domains of Tudor domain-containing protein TDRD7

Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, MN, 55905, USA.
Biomolecular NMR Assignments (Impact Factor: 0.82). 04/2012; 7(1). DOI: 10.1007/s12104-012-9382-1

ABSTRACT The LOTUS or OST-HTH domain is a recently discovered motif of about 80 amino acids and is found in several germline-specific proteins including the Tudor domain-containing proteins TDRD5 and TDRD7, which are important for germ cell development. The LOTUS domain is an RNA binding domain but its exact function is unknown. Here, we report the 1H, 13C and 15N resonance assignments for the three LOTUS domains present in mouse TDRD7. These assignments will allow three-dimensional structure determination of the LOTUS domains and mapping of their interaction with RNA, steps toward deciphering the function of TDRD7.

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