The effect of domestication on a brown trout (Salmo trutta m fario) broodstock in Hungary

Aquaculture International (Impact Factor: 0.98). 02/2014; 22(1). DOI: 10.1007/s10499-013-9665-2


Molecular markers (PCR–RFLP and microsatellite) were used to investigate the genetic background of the only brown trout (Salmo trutta m fario) broodstock in Hungary which due to the hydrogeography of the country should theoretically belong to the Danubian lineage. PCR–RFLP (mitochondrial DNA control region and lactate dehydrogenase and somatolactin genes) as well as microsatellite (BFRO002, OMM1064, Ssa408uos, SsoSL417, SsoSL438) markers were used to distinguish between Danubian and Atlantic lineages of brown trout in the Lillafüred broodstock. Altogether 435 fish were tagged during the experimental period. Due to mortalities, fin clips were collected from 401 individuals. According to the genetic analysis of the mitochondrial DNA, the Danubian haplotype is present in only one individual (0.2 %) of the broodstock. Analysis of the nuclear markers revealed that alleles characteristic of both the Atlantic and the Danubian lineages are found in the population. However, Atlantic alleles dominate throughout the broodstock which is in Hardy–Weinberg equilibrium according to the investigated markers. Results indicate that the original broodstock that was introduced to the farm following its construction in 1933 was of the Atlantic lineage. Although later fish from a local stream were collected and added to the broodstock, the number of these was limited and they were almost exclusively males. Fish from this farm that are stocked by anglers can have a significant genetic impact on trout populations of natural streams.

Download full-text


Available from: Ákos Horváth, Jul 21, 2014
58 Reads
  • Source
    • "This has raised concerns that introductions of brown trout of Atlantic and Danube linage into the river catchment may be leading to the extinction of trout species endemic to the River Jadro but also to the Adriatic basin (Snoj et al. 2008; Mrdak et al. 2012). Supportive evidence for this can be found in the River Danube basin, that is introgression of alien At1 haplotype of sea trout (Mari c et al. 2006, 2010, 2012), which caused the loss of intraspecific variability after the introduction of non-native strains and a change in genetic composition of native brown trout stock of Danube lineage (Horv ath et al. 2014; Simonovi c et al. 2014). "
    [Show abstract] [Hide abstract]
    ABSTRACT: In this first application to Croatia and Slovenia, the Fish Invasiveness Screening Kit (FISK) version 2 was used to assess the invasiveness potential of 40 introduced and translocated freshwater fish species. Based on a priori classification of invasiveness, ROC analysis of FISK scores from two independent assessors resulted in a statistically significant calibration threshold of 11.75. This indicated that FISK was able to discriminate reliably between non-native species likely to pose a high risk of being invasive and those likely to pose a medium or low risk of invasiveness. Seven species were categorised as ‘medium risk’ and the other 33 as ‘high risk’, whereas no species were categorised as ‘low risk’. The two highest scoring species were European catfish and North African catfish. Mean scores for all species classified a priori as invasive were ranked as ‘high risk’ sensu lato and fell into the ‘moderately high risk’ sub-category. FISK proved to be a useful and viable tool for assessing the risks posed by non-native fishes in Croatia and Slovenia. For this reason, it can be adopted as a reliable tool for the prevention of new translocations or introductions of potentially invasive species in the risk assessment area, as well as to assist in decisions regarding future management (i.e. monitoring, control and eradication) and conservation strategies.
    Fisheries Management and Ecology 07/2015; · 1.76 Impact Factor