Spectrophotometric determination of Chlorpheniramine Maleate and Phenylpropanolamine Hydrochloride by “two wavelengths method”

Journal of Pharmacy Research (Impact Factor: 2.89). 05/2013; 7(5):404–408. DOI: 10.1016/j.jopr.2013.04.043


Early methods for simultaneous determination of Chlorpheniramine Maleate (CPM) and Phenylpropanolamine Hydrochloride (PPM) in combined pharmaceutical dosage forms are expensive and time consuming methods, so this study has been performed for simultaneous determination by using UV spectrophotometer to save excessive cost and time.MethodA rapid and simple method for simultaneous determination of Chlorpheniramine Maleate (CPM) and Phenylpropanolamine Hydrochloride (PPM) by “Two Wavelengths Method” using UV spectrophotometer has been developed in combined pharmaceutical dosage forms. “The absorbance difference between two points on the mixture spectra is directly proportional to the concentration of the component of interest independent of interfering component”. For selection of two wavelengths for estimation of PPM at 257 nm it showed remarkable absorbance (λmax of PPM) which was noted and another point where it showed equal absorbance to that of 257 nm was reviewed over the curve and was found out as 263.6 nm. For selection of two wavelengths for estimation of CPM, at 261.6 nm (λmax of CPM) it showed remarkable absorbance. Another point where it showed equal absorbance to that of 261.6 nm was reviewed over the curve and was found out as 253.2 nm.Results and discussionIn the present research work an attempt has been made to develop simple method of analysis for combination of Phenylpropanolamine Hydrochloride and Chlorpheniramine Maleate as literature review revealed that no other simple reported method except HPLC, which requires sophisticated instrument and HPLC grade solvents. This method presented above utilizes the absorbance of ultraviolet radiations by PPM and CPM, distilled water was the solvent employed for this method. This method is advantageous as requires less memory capacity for storage of calibration data as well as less time consuming as compare to multicomponent analysis by other instruments.Conclusion
The most striking features of “Two Wavelengths Method” are its simplicity, sensitivity and rapidity. It is also an easier and economical method than HPLC separation technique and does not require the use of any expensive or toxic reagent. These advantages make it especially suitable for routine quality control.

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