Spectrophotometric determination of Chlorpheniramine Maleate and Phenylpropanolamine Hydrochloride by “two wavelengths method”
Early methods for simultaneous determination of Chlorpheniramine Maleate (CPM) and Phenylpropanolamine Hydrochloride (PPM) in combined pharmaceutical dosage forms are expensive and time consuming methods, so this study has been performed for simultaneous determination by using UV spectrophotometer to save excessive cost and time.MethodA rapid and simple method for simultaneous determination of Chlorpheniramine Maleate (CPM) and Phenylpropanolamine Hydrochloride (PPM) by “Two Wavelengths Method” using UV spectrophotometer has been developed in combined pharmaceutical dosage forms. “The absorbance difference between two points on the mixture spectra is directly proportional to the concentration of the component of interest independent of interfering component”. For selection of two wavelengths for estimation of PPM at 257 nm it showed remarkable absorbance (λmax of PPM) which was noted and another point where it showed equal absorbance to that of 257 nm was reviewed over the curve and was found out as 263.6 nm. For selection of two wavelengths for estimation of CPM, at 261.6 nm (λmax of CPM) it showed remarkable absorbance. Another point where it showed equal absorbance to that of 261.6 nm was reviewed over the curve and was found out as 253.2 nm.Results and discussionIn the present research work an attempt has been made to develop simple method of analysis for combination of Phenylpropanolamine Hydrochloride and Chlorpheniramine Maleate as literature review revealed that no other simple reported method except HPLC, which requires sophisticated instrument and HPLC grade solvents. This method presented above utilizes the absorbance of ultraviolet radiations by PPM and CPM, distilled water was the solvent employed for this method. This method is advantageous as requires less memory capacity for storage of calibration data as well as less time consuming as compare to multicomponent analysis by other instruments.Conclusion
The most striking features of “Two Wavelengths Method” are its simplicity, sensitivity and rapidity. It is also an easier and economical method than HPLC separation technique and does not require the use of any expensive or toxic reagent. These advantages make it especially suitable for routine quality control.
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ABSTRACT: Acetaminophen, phenylephrine and chlorpheniramine are frequently associated in pharmaceutical formulations against the common cold. Their quantification presents several problems. A HPLC method for the simultaneous determination of these compounds in pharmaceutical formulations such as capsules and sachets, including the separation of impurities and excipients has been developed and validated. The selectivity of the method was also tested to be used if phenylpropanolamine hydrochloride were employed instead of phenylephrine. Final chromatographic conditions were a gradient elution, being solvent A: phosphate buffer 40 mM at pH 6.0 and solvent B: acetonitrile. At t=0, the mobile phase consisted of 92% A and 8% B and it changed with a linear gradient during 8 min to 75% A and 25% B. At min 8, it changed to 30% A and 70% B for 5 min and at t=15 min, it returns to the initial conditions (92% A and 8% B) during 1 min remaining at this composition until t=20 min. UV detection was performed at 215 nm for phenylephrine and chlorpheniramine, because at this wavelength sensitivity was higher than in other more characteristic wavelengths and it was necessary for the detection of minor compounds. For acetaminophen 280 nm was employed. Validation parameters permit to consider the method adequate.Journal of Pharmaceutical and Biomedical Analysis 08/2002; 29(4):701-14. DOI:10.1016/S0731-7085(02)00124-3 · 2.83 Impact Factor
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ABSTRACT: A sensitive enantioselective high-performance liquid chromatographic (HPLC) method has been developed for the simultaneous determination of plasma concentrations of (-)(R)- and (+)(S)-chlorpheniramine (CP) and their metabolites, desmethyl-chlorpheniramine (DCP), didesmethyl-chorpheniramine (DDCP) and chlorpheniramine N-oxide (CPNO). Enantioselective separations were achieved on a beta-cyclodextrin chiral stationary phase (CYCLOBOND I 2000) with a mobile phase consisting of diethylamine acetate (0.25%, pH 4.4):methanol:acetonitrile [85:7.5:7.5, (v/v/v)]and a flow-rate of 0.5 ml/min. For CP, the enantioselectivity (alpha) of the separation was 1.12 with a resolution factor (R(s)) of 1.17. The method was validated for CP by using mass spectroscopy detection (MSD). Concentrations of each enantiomer could be measured down to 125 pg/ml from a 1-ml plasma sample. Extracted calibration curves were linear from 0.13 to 50.00 ng/ml for each enantiomer. The method was applied to samples from two clinical studies.Journal of Pharmaceutical and Biomedical Analysis 02/2002; 27(3-4):479-88. DOI:10.1016/S0731-7085(01)00570-2 · 2.83 Impact Factor
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ABSTRACT: The objective of this article is to review the methodologies of determination of the most widely used analytes in cough and cold preparations by HPLC. This article studies the effect of all chromatographic parameters so as to provide a fast, reliable and cost effective methodology of testing. KEY WORDS-High Performance Liquid Chromatography (HPLC), Over the Counter (OTC).