Manufacturing and wetting low-cost microfluidic cell separation devices

Biomicrofluidics (Impact Factor: 3.36). 09/2013; 7:056501. DOI: 10.1063/1.4821315


Deterministic lateral displacement (DLD) is a microfluidic size-based particle separation or filter technology with applications in cell separation and enrichment. Currently, there are no cost-effective manufacturing methods for this promising microfluidic technology. In this fabrication paper, however, we develop a simple, yet robust protocol for thermoplastic DLD devices using regulatory-approved materials and biocompatible methods. The final standalone device allowed for volumetric flow rates of 660 ll min À1 while reducing the manufacturing time to <1 h. Optical profilometry and image analysis were employed to assess manufacturing accuracy and precision; the average replicated post height was 0.48% less than the average post height on the master mold and the average replicated array pitch was 1.1% less than the original design with replicated posts heights of 62.

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Available from: Ryan S Pawell, Oct 04, 2015
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    ABSTRACT: [This corrects the article on p. 056501 in vol. 7.].
    Biomicrofluidics 09/2013; 7(5):59901. DOI:10.1063/1.4827599 · 3.36 Impact Factor
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