Manufacturing and wetting low-cost microfluidic cell separation devices

Biomicrofluidics (Impact Factor: 3.77). 09/2013; 7:056501. DOI: 10.1063/1.4821315

ABSTRACT Deterministic lateral displacement (DLD) is a microfluidic size-based particle separation or filter technology with applications in cell separation and enrichment. Currently, there are no cost-effective manufacturing methods for this promising microfluidic technology. In this fabrication paper, however, we develop a simple, yet robust protocol for thermoplastic DLD devices using regulatory-approved materials and biocompatible methods. The final standalone device allowed for volumetric flow rates of 660 ll min À1 while reducing the manufacturing time to <1 h. Optical profilometry and image analysis were employed to assess manufacturing accuracy and precision; the average replicated post height was 0.48% less than the average post height on the master mold and the average replicated array pitch was 1.1% less than the original design with replicated posts heights of 62.

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    ABSTRACT: Micropost arrays serve as a plaform for the next generation of diagnostic devices. These arrays are found in microfluidic devices for peripheral blood-based diagnostics and metastatic cancer management. The function and performance of these devices is determined by the underlying micro-scale fluid mechanics. Typically, these devices operate in the creeping regime (Re << 1) where the viscous forces of the fluids dominate. Recent advances in manufacturing allow for higher Reynolds number flows (Re >> 1) where the inertial forces dominate. In this work, we use computational simulations to show there is a transitional region (1 < Re < 20) in between the laminar and creeping regimes for two different micropost array geometries. Numerical analysis is employed to investigate jet formation both within the array and at the array exit. The peak-to-peak amplitude of the streamwise normalized velocity profile is used to quantify jet formation within the array; the streamwise velocity profile at the end of the array exit is used to determine jet length at the exit of the array. Above the transitional region (Re > 20) significant jets form downstream of the posts, amplitude scales exponentially and jet length scales with Re according to power law.
    05/2014; 553:367-372. DOI:10.4028/
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    ABSTRACT: [This corrects the article on p. 056501 in vol. 7.].
    Biomicrofluidics 09/2013; 7(5):59901. DOI:10.1063/1.4827599 · 3.77 Impact Factor


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May 22, 2014