DNA-directed self-assembly of shape-controlled hydrogels.

1] Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, Massachusetts 02115, USA [2] Department of Medicine, Center for Biomedical Engineering, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA [3] Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.
Nature Communications (Impact Factor: 10.74). 09/2013; 4:2275. DOI: 10.1038/ncomms3275
Source: PubMed

ABSTRACT Using DNA as programmable, sequence-specific 'glues', shape-controlled hydrogel units are self-assembled into prescribed structures. Here we report that aggregates are produced using hydrogel cubes with edge lengths ranging from 30 μm to 1 mm, demonstrating assembly across scales. In a simple one-pot agitation reaction, 25 dimers are constructed in parallel from 50 distinct hydrogel cube species, demonstrating highly multiplexed assembly. Using hydrogel cuboids displaying face-specific DNA glues, diverse structures are achieved in aqueous and in interfacial agitation systems. These include dimers, extended chains and open network structures in an aqueous system, and dimers, chains of fixed length, T-junctions and square shapes in the interfacial system, demonstrating the versatility of the assembly system.

  • [Show abstract] [Hide abstract]
    ABSTRACT: We have created a selective macroscopic self-assembly process by using polymer gels modified with complementary DNA oligonucleotides or nucleobases. The hydrogels modified with complementary DNA oligonucleotides adhered to each other by simple contact. The organogels modified with complementary nucleobases selectively formed macroscopic assemblies by agitation in nonpolar organic solvents. The adhesion strength of each gel was estimated semi-quantitatively by stress–strain measurements. We achieved direct adhesion between macroscopic materials both in water and in organic media, based on complementary hydrogen bonds.
    Chemistry - A European Journal 12/2014; 21(7). DOI:10.1002/chem.201404674 · 5.70 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Stimuli-responsive (smart) hydrogels have attracted widespread attention as biomimetic systems due to their ability to respond to subtle changes in external and internal stimuli ranging from physical triggers such as temperature and electric field to chemical triggers like glucose and pH. Besides their intriguing behavior, the main interest in such smart hydrogels lies in their potential industrial and biomedical appli-cations. Some of these applications include injectable biomaterials, tunable surfaces for cell sheet engin-eering, sensors, and actuators. In this review, we discuss the fundamental principles underlying the stimuli-responsive behavior of hydrogels and how these properties have led to major technological inno-vations. We also review recent advancements in the field of hydrogels, including self-healing and stimuli-responsive degradation in hydrogels. We conclude by providing a perspective on the potential use of smart hydrogels as multifunctional, bioactuating systems for cell and tissue engineering.
    02/2014; 2(5):603-618. DOI:10.1039/c3bm60288e
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: The development of cell based Advanced Therapeutic Medicinal Products (ATMPs) for bone repair has been expected to revolutionize the health care system for the clinical treatment of bone defects. Despite this great promise, the clinical outcomes of the few cell based ATMPs that have been translated into clinical treatments have been far from impressive. In part, the clinical outcomes have been hampered because of the simplicity of the first wave of products. In response the field has set-out and amassed a plethora of complexities to alleviate the simplicity induced limitations. Many of these potential second wave products have remained “stuck” in the development pipeline. This is due to a number of reasons including the lack of a regulatory framework that has been evolving in the last years and the shortage of enabling technologies for industrial manufacturing to deal with these novel complexities. In this review, we reflect on the current ATMPs and give special attention to novel approaches that are able to provide complexity to ATMPs in a straightforward manner. Moreover, we discuss the potential tools able to produce or predict ‘Goldilocks’ ATMPs, which are neither too simple nor too complex.
    Advanced Drug Delivery Reviews 11/2014; DOI:10.1016/j.addr.2014.10.025 · 12.71 Impact Factor

Full-text (2 Sources)

Available from
May 21, 2014