A New Player in Environmentally Induced Oxidative Stress: Polychlorinated Biphenyl Congener, 3,3' dichlorobiphenyl (PCB11).

Free Radical and Radiation Biology Program, B180 Medical Laboratories, Department of Radiation Oncology, Holden Comprehensive Cancer Center, University of Iowa, Iowa City, Iowa 52242, USA.
Toxicological Sciences (Impact Factor: 3.85). 08/2013; 136(1). DOI: 10.1093/toxsci/kft186
Source: PubMed


Recent analysis of air samples from Chicago and Lake Michigan areas observed a ubiquitous airborne PCB congener, 3,3'-dichlorobiphenyl (PCB 11). Our analysis of serum samples also revealed the existence of hydroxylated metabolites of PCB11 in human blood. Because, PCBs and PCB metabolites have been suggested to induce oxidative stress, this study sought to determine whether environmental exposure to PCB11 and its 4-hydroxyl metabolite could induce alterations in steady-state levels of reactive oxygen species (ROS) and cytotoxicity in immortalized human prostate epithelial cells (RWPE-1). This study also examines if antioxidants could protect the cells from PCB11 induced cytotoxicity. Exponentially growing RWPE-1 cells were exposed to 3 μM PCB 11 and its metabolite, 3,3'-dichlorobiphenyl-4-ol (4-OH-PCB11) as well as an airborne PCB mixture resembling the Chicago ambient air congener profile, everyday for 5 days. Results showed that 4-OH-PCB11 could significantly induce cell growth suppression and decrease the viability and plating efficiency of RWPE-1 cells. 4-OH-PCB11 also significantly increased steady-state levels of intracellular O2(•) as well as hydroperoxides. Finally, treatment with the combination of polyethylene glycol conjugated CuZnSOD and catalase added 1 hour after 4-OH-PCB11 exposures, significantly protected RWPE-1 cells from PCB toxicity.The results strongly support the hypothesis that exposure to a hydroxylated metabolite of PCB11 can inhibit cell proliferation and cause cytotoxicity by increasing steady state levels of ROS. Furthermore, antioxidant treatments following PCBs exposure could significantly mitigate the PCB-induced cytotoxicity in exponentially growing human prostate epithelial cells.

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    • "Several studies have reported that some PCBs and their metabolites alter cellular glutathione homeostasis, and that NAC co-treatment can attenuate adverse effects in cells in culture and in vivo following PCB exposure (Lai et al. 2010; Slim et al. 2000; Srinivasan et al. 2001; Zhu et al. 2013). "
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    Environmental Science and Pollution Research 07/2015; DOI:10.1007/s11356-015-4987-4 · 2.83 Impact Factor
    • "Despite this knowledge, human uptake, metabolism, and toxic response to PCB 11 and other nonlegacy PCBs remains poorly understood. Initial laboratory studies with animals and plants have demonstrated the potential of PCB 11 to become bioavailable to humans through inhalation and to be accessible for biotransformation (Hu et al. 2010b, 2013, 2012, Hu et al. 2014, Zhu et al. 2013). In 2013, PCB 11 was reportedly detected in human serum for the fi rst time, thereby further emphasizing the need for a better understanding of the exposure , metabolism, and toxicities of nonlegacy PCBs (Marek et al. 2013b). "
    Science 09/1991; 253(5026). DOI:10.1126/science.253.5026.1334 · 33.61 Impact Factor
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