Imaging Nascent RNA Dynamics in Dictyostelium
MRC Laboratory for Molecular Cell Biology, Department of Cell and Developmental Biology, University College London, London, UK.Methods in molecular biology (Clifton, N.J.) (Impact Factor: 1.29). 08/2013; 1042:101-13. DOI: 10.1007/978-1-62703-526-2_8
Dictyostelium cells have great utility for live imaging of single gene transcriptional dynamics. The cells allow efficient molecular genetics, for targeting of RNA reporters and fluorescent proteins to individual, defined loci. Dictyostelium cells share many signalling, chromatin and nuclear characteristics of larger eukaryotes, yet the cells have a relatively simple scattered differentiation programme, allowing imaging of transcriptional events in the context of stochastic developmental choices. This review will detail the methods and considerations for imaging nascent RNA dynamics at single genes in living Dictyostelium cells.
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