[Show abstract][Hide abstract] ABSTRACT: A method for distinguishing isolates of the fungi belonging to genus Cordyceps, used as
food/medicine in Eastern Asia, was developed. A PCR-single stranded conformation
polymorphism-based profile was applied and established on the ITS2 region of rDNA
for molecular typing in 48 different isolates of Cordyceps species. Compared with the
DNA sequencing data and secondary structure prediction of single strand DNA, the
method used was sensitive and could distinguish between two closely related species in
the genus Cordyceps. This method could be very useful for confirming the identity of a
specific strain of Cordyceps for researchers and food companies that produce the fermented
Cordyceps products as a quality control method.
[Show abstract][Hide abstract] ABSTRACT: Cultivation characteristics of fruit-body (synnema) formation ofIsaria japonica were examined using liquid and solid media in order to produce fruit-bodies on a large scale. Mycelia grew well at 18–28°C
on PDA medium with an initial pH of 7.0. The formation of fruit-bodies ofI. japonica was induced by lowering temperature to below 20°C in PD liquid medium. In sawdust-rice bran basal medium mixed with pupal
powder prepared from silkworms (Bombyx mori), the fresh weight of fruit-bodies increased with increasing content of pupal powder. The highest yields of fruit-bodies
were obtained in carbon-rich barley grain medium supplemented with pupal powder. The fruit-bodies grown under CO2 concentrations of 1,000 μl/L had coral-like, many-branched synnemata with numerous conidiospores, whereas those formed under
high concentrations (9,000 μl/L) of CO2 had unbranched and longer synnemata. High concentrations of CO2 remarkably inhibited conidiospore formation on synnemata. Continuous high-intensity illumination at 2.93 W·m−2 inhibited the elongation of synnemata, and low-intensity illumination at 0.088 W·m−2 slightly inhibited the branching of synnemata. Fruit-bodies were produced on the pupa metamorphosed from living larvae ofAgrotis fucosa placed on the surface of a culture ofI. japonica incubated in sawdust-rice bran medium.
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