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Tochukaso (Semitake and others), Cordyceps species

Food Reviews International (Impact Factor: 2.54). 02/1995; 11(1):231-234. DOI: 10.1080/87559129509541038
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    • "Cordyceps, the entomopathogenic fungi, have long been used as food/herbal medicines in Eastern Asia (Kiho and Ukai, 1995). Some Cordyceps spp., for example , C. cicadicola, C. liangshanensis, C. hawkesii, C. militaris, C. ophioglossoides, C. shanxiensis and C. sobolifera (Shiu, 1997) have been demonstrated to produce natural products with various biological activities (Huang et al., 2003) that can be used as medicines or functional food ingredients. "
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    ABSTRACT: A method for distinguishing isolates of the fungi belonging to genus Cordyceps, used as food/medicine in Eastern Asia, was developed. A PCR-single stranded conformation polymorphism-based profile was applied and established on the ITS2 region of rDNA for molecular typing in 48 different isolates of Cordyceps species. Compared with the DNA sequencing data and secondary structure prediction of single strand DNA, the method used was sensitive and could distinguish between two closely related species in the genus Cordyceps. This method could be very useful for confirming the identity of a specific strain of Cordyceps for researchers and food companies that produce the fermented Cordyceps products as a quality control method.
    Food Biotechnology 11/2008; 22(4):311-325. DOI:10.1080/08905430802458354 · 0.64 Impact Factor
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    • "They affect metabolism, the immune and endocrine systems, and reportedly enhance the body's resistance to inflammation and cancer (Wang, 1995). Chemical studies on C. sinensis have shown the presence of various polysaccharides, nucleosides, cyclodepsipeptides, alkaloids, mannitol, vitamins, trace minerals and various enzymes (Li et al., 2003, 2004a, b; Huang et al., 2003; Cai et al., 2003; Halpern, 1999; Mizuno, 1999; Kiho et al., 1999; Zhu, et al., 1998a, b; Shen et al., 1998; Kiho and Ukai, 1995; Wang, 1995; Kuo et al., 1994; Zhi et al., 1991; Miyazaki et al., 1977; Tang, 1947). "
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    ABSTRACT: The compositions of natural fruiting bodies of Cordyceps sinensis (NFCS), mycelia from submerged culture (MSMC) and shake culture (MSKC) of RCEF0273, a strain of Hirsutella sinensis, anamorph of C. sinensis, were compared to evaluate the potentiality of MSMC. The contents of crude fat, crude protein, total and essential amino acids were in the following descending order: MSKC>MSMC>NFCS, respectively. Unsaturated fatty acids of MSMC accounted for 65.9% of total fatty acids, obviously lower than those of NFCS (86.9%) and MSKC (76.5%). Both As and Hg were lower than 0.20 μg/g in all samples tested. The contents of Ca, Se and Cu were higher in MSMC than in NFCS, while the contents of Fe, Zn and Sn, were lower in MSMC than in NFCS. Vitamin B6 was not detected in MSMC but in NFCS, while vitamin B1 was not found in NFCS but in freeze-dried MSMC. Niacin content of NFCS was 1.6 times more than that of MSMC. The total content of four nucleosides (adenosine, guanosine, uridnine and inosine) in MSMC (6.20 mg/g) was significantly higher than those of NFCS (1.80 mg/g) and MSKC (1.60 mg/g). The above results suggest that MSMC can probably be used as a substitute for NFCS.
    Journal of Food Composition and Analysis 12/2006; 19(8-19):800-805. DOI:10.1016/j.jfca.2006.04.007 · 2.26 Impact Factor
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    • "Some Cordyceps sp. have long been used to promote longevity, relieve exhaustion and treat numerous illnesses by acting as a hemostatic, a mycolytic, an anti-asthmatic and a hypoglycemic agents in Chinese traditional medicines [1] [2] [3]. They could produce many kinds of bioactive compounds such as cordycepin, ophiocordin and some polysaccharides [4] [5] [6] [7] [8] [9]. Cordycepin (3 -deoxyadenosine) is a nucleoside analogue, which has a broad spectrum of biological activity. "
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    ABSTRACT: The production conditions of cordycepin (3′-deoxyadenosine), which is a kind of nucleoside analogue, were investigated by a surface culture using Cordyceps militaris NBRC 9787. About 98% of the cordycepin synthesized by Cordyceps militaris was secreted into the culture medium, and other nucleic acid-related compounds except for cordycepin and guanine were only slightly observed in the medium. The preferable nitrogen source for the cordycepin production was the mixture of peptone and yeast extract, in which the ratio of the yeast extract was 75% or greater. The optimal carbon/nitrogen ratio (w/w) was 2/1 when glucose was used as the carbon source. The lower medium depth resulted in a higher productivity over the present experimental range from 1.8 to 5.3 cm. Under the optimal conditions, the maximum cordycepin concentration in the culture medium reached 640 mg/l, and the maximum cordycepin productivity was 32 mg/(l·day).
    Enzyme and Microbial Technology 08/2006; 39(4-39):641-646. DOI:10.1016/j.enzmictec.2005.11.010 · 2.97 Impact Factor
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