"Cordyceps species, including cicadicolous fungi such as
O. sobolifera, are regarded as medicinal mushrooms in oriental society [2, 8-11]. In this context, many researchers have begun to study cultivation characteristics of Cordyceps and allied species [5, 12-23]. "
[Show abstract][Hide abstract] ABSTRACT: We investigated the effect of nutritional and environmental factors on Ophiocordyceps longissima mycelial growth. The longest colony diameter was observed on Schizophyllum (mushroom) genetics complete medium plus yeast extract, Schizophyllum (mushroom) genetics minimal medium, and Sabouraud dextrose agar (SDA); however, malt-extract yeast-extract agar, SDA plus yeast extract, yeast-extract malt-extract peptone dextrose agar, SDA, oatmeal agar, and potato dextrose agar showed higher mycelia density. A temperature of 25℃ was optimum and 7.0 was the optimum pH for mycelial growth. Colony diameter was similar under light and dark conditions. Maltose and yeast extract showed the highest mycelial growth among carbon and nitrogen sources respectively. The effect of mineral salts was less obvious; however, K(3)PO(4) showed slightly better growth than that of the other mineral salts tested. Among all nutrition sources tested, complex organic nitrogen sources such as yeast extract, peptone, and tryptone were best for mycelial growth of O. longissima. Ophiocordyceps longissima composite medium, formulated by adding maltose (2% w/v), yeast extract (1% w/v), and K(3)PO(4) (0.05% w/v) resulted in slightly longer colony diameter. In vitro mycelial O. longissima growth was sustainable and the production of fruiting bodies could be used for commercial purposes in the future.
"Cordyceps, the entomopathogenic fungi, have long been used as food/herbal medicines in Eastern Asia (Kiho and Ukai, 1995). Some Cordyceps spp., for example , C. cicadicola, C. liangshanensis, C. hawkesii, C. militaris, C. ophioglossoides, C. shanxiensis and C. sobolifera (Shiu, 1997) have been demonstrated to produce natural products with various biological activities (Huang et al., 2003) that can be used as medicines or functional food ingredients. "
[Show abstract][Hide abstract] ABSTRACT: A method for distinguishing isolates of the fungi belonging to genus Cordyceps, used as
food/medicine in Eastern Asia, was developed. A PCR-single stranded conformation
polymorphism-based profile was applied and established on the ITS2 region of rDNA
for molecular typing in 48 different isolates of Cordyceps species. Compared with the
DNA sequencing data and secondary structure prediction of single strand DNA, the
method used was sensitive and could distinguish between two closely related species in
the genus Cordyceps. This method could be very useful for confirming the identity of a
specific strain of Cordyceps for researchers and food companies that produce the fermented
Cordyceps products as a quality control method.
"Some Cordyceps sp. have long been used to promote longevity, relieve exhaustion and treat numerous illnesses by acting as a hemostatic, a mycolytic, an anti-asthmatic and a hypoglycemic agents in Chinese traditional medicines   . They could produce many kinds of bioactive compounds such as cordycepin, ophiocordin and some polysaccharides      . Cordycepin (3 -deoxyadenosine) is a nucleoside analogue, which has a broad spectrum of biological activity. "
[Show abstract][Hide abstract] ABSTRACT: The methods for increasing the production of cordycepin (3′-deoxyadenosine), which is a kind of nucleoside analogue, were investigated by a surface culture of Cordyceps militaris NBRC 9787 using liquid medium. As a result of adding various compounds such as purine biosynthesis-related compounds, coenzymes and surfactant to the basal medium for the purpose of enhancing the cordycepin production, glycine, l-aspartic acid, l-glutamine, adenine and adenosine were shown to be effective additives. The most preferable condition of the additives was the combination of 1 g/l of adenine and 16 g/l of glycine, and the maximum cordycepin production reached 2.5 g/l, which corresponds to 4.1 times that in the basal medium, and this value was higher compared to other reports. As a more efficient method, the repeated batch operation was shown to be practicable, and the productivity by the repeated batch operation in the optimal medium reached 0.19 g/(l d), which corresponds to 5.5 times that by the batch operation in the basal medium. The nucleic acid-related compounds produced by the surface culture were also analyzed. The results indicated that about 97% of the cordycepin synthesized by C. militaris was excreted into the culture medium, and the production of cordycepin and guanine might be linked to each other.
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