Taxonomic revision of Eurotium and transfer of species to Aspergillus

Botany, Faculty of Science, Charles University in Prague, Benátská 2, 128 01, Praha 2, Benátská 2, Prague 2, 12801, Czech Republic.
Mycologia (Impact Factor: 2.13). 07/2013; 105(4):912-937. DOI: 10.3852/12-151
Source: PubMed

ABSTRACT Aspergillus section Aspergillus contains economically important, xerophilic fungi that are widely distributed in nature and the human environ-ment and are known for their ability to grow on substrates with low water activity. The taxa were revised based on sequence data from four loci, PCR fingerprinting, micro-and macromorphology, and physiology. The number of taxa was reduced to 17 species, all of which can be distinguished with sequence data from either the caM or RPB2 locus. The original description of A. proliferans was supple-mented by a description of its teleomorph. This species seems to be relatively common and often has been confused with A. glaucus. In addition, green sporulating isolates of A. niveoglaucus isolated from food and several other substrates are indistinguish-able in phenotype from A. glaucus. A dichotomous key based on ascospore size and ornamentation and the ability to grow at specific combinations of temperature and water activity is provided for identification of species. In response to recent changes in the botanical code, we transferred the Eurotium species to Aspergillus and selected one name for each species.

  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: The molecular genetic methods (MGM) are used frequently in clinical microbiology to identify the agent of infection and also to its early direct detection in clinical specimens. In dermatomycology MGM supply or even substitute the classic time consuming and sophisticated diagnostic methods based on direct microscopy and cultivation. The high sensitivity and rapid agent detection available in few hours or days is an advantage of MGM that enables to start the early targeted therapy or to avoid an unnecessary antifungal treatment. However MGM designated to direct micromycetes detection does not guarantee their precise diagnostics which still requires the pathogen isolation before MGM are used to its identification. Article reviews the possibilities of MGM use in direct dermatophytes detection from clinical specimens and in species and strains identification.
    Ceskoslovenská dermatologie 08/2014; 89(4):175-186.
  • [Show abstract] [Hide abstract]
    ABSTRACT: A new species from Guangdong Province in south China, Polycephalomyces lianzhouensis, is described and illustrated here; this species was found to co-occur with Ophiocordyceps crinalis. The phylogenetic position of this species was investigated according to morphological features and phylogenetic inferences based on the nrSSU, nrLSU, tef1, rpb1, and ITS gene sequences. Results demonstrated that P. lianzhouensis was highly associated with P. ramosopulvinatus but could be distinguished from the latter using the sequences of rpb1, nrLSU, and ITS gene and the features of much smaller stromata and perithecia and longer part-spores.
    Mycological Progress 11/2014; 13(4-4):1089-1096. DOI:10.1007/s11557-014-0996-9 · 1.54 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: The aim of the study was to evaluate the spectrum of causative agents of the main clinical forms of dermatophytoses in the Czech Republic by molecular genetic methods (MGM). During two years (from July 2011 to June 2013), 3255 cultivation specimens were positive for dermatophytes. The highest number of specimens was isolated from tinea unguinum (55,5%), then tinea corporis (29,2%), tinea pedis (14,6%) and tinea capitis (0,7%). The identification of isolated species (n = 672) except for Trichophytom rubrum (n = 2563) was performed by MGM (PCR fingerprinting or sequencing of ITS segments of rDNA). For T. rubrum only morphologically non-clear isolates were identified (n = 189). In total, 14 species of dermatophytes were identified. The most important change noticed compared to previous results was an increased detection of Arthroderma benhamiae in the cases of tinea corporis (22,9%) and tinea capitis (29,2%). Geophilic species caused only 1,3% of all infections even if this group comprised the highest number of species with two newly described ones and also Microsporum persicolor and M. fulvum wich are usually overlooked in the morphological examination.
    Ceskoslovenská dermatologie 08/2014; 89(4):167-174.


Available from
Jul 11, 2014