Taxonomic revision of Eurotium and transfer of species to Aspergillus

Botany, Faculty of Science, Charles University in Prague, Benátská 2, 128 01, Praha 2, Benátská 2, Prague 2, 12801, Czech Republic.
Mycologia (Impact Factor: 2.47). 07/2013; 105(4):912-937. DOI: 10.3852/12-151
Source: PubMed


Aspergillus section Aspergillus contains economically important, xerophilic fungi that are widely distributed in nature and the human environ-ment and are known for their ability to grow on substrates with low water activity. The taxa were revised based on sequence data from four loci, PCR fingerprinting, micro-and macromorphology, and physiology. The number of taxa was reduced to 17 species, all of which can be distinguished with sequence data from either the caM or RPB2 locus. The original description of A. proliferans was supple-mented by a description of its teleomorph. This species seems to be relatively common and often has been confused with A. glaucus. In addition, green sporulating isolates of A. niveoglaucus isolated from food and several other substrates are indistinguish-able in phenotype from A. glaucus. A dichotomous key based on ascospore size and ornamentation and the ability to grow at specific combinations of temperature and water activity is provided for identification of species. In response to recent changes in the botanical code, we transferred the Eurotium species to Aspergillus and selected one name for each species.

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Available from: Vit Hubka, Jul 11, 2014
    • "Morphological and cultural observations were performed for isolates whose DNA sequences were uninformative. Particularly, the keys developed by Frisvad and Samson (2004) were used for Penicillium isolates, whereas identication of isolates belonging to genus Aspergillus was performed according to the keys developed by Hubka et al. (2013). "
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    • "Genomic DNA was extracted from 4-weeks-old cultures using the ArchivePure DNA yeast and Gram2 ? kit (5PRIME Inc., Gaithersburg, MD, U.S.A.) with modifications described by Hubka et al. (2013). The ITS region of rDNA was amplified with primers ITS1 and ITS4 (White et al. 1990); nuc28S with primers NL1 (5 0 -GCATATCAATAAGCGGAG- GAAAAG) and LR6 (Vilgalys and Hester 1990); nuc18S with primers with primers NS1 (White et al. 1990) and NS24 (Gargas and Taylor 1992); and partial b-tubulin gene with primers Ben2f (Hubka and Kolařík 2012) and Bt2b (Glass and Donaldson 1995). "
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    • "Eurotium species are xerophiles and are commercially important because many species are associated with spoilage, not only of food commodities but also of art and artefacts, paper, leather goods, textiles, timber, even lens of cameras, microscopes and binoculars. Eurotium is widely accepted among user groups, such that reintroduction of Aspergillus would cause widespread confusion, especially because many names would have to changed, as shown in Hubka et al. (2013). "
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