Preparation and Porous Property of C 14 Monolithic Column for Capillary Electrochromatography

Laboratory of Electrochemistry and Analytical Chemistry UMR CNRS 7575-ENSCP 11 Rue Pierre et Marie Curie 75231 Paris Cedex 05 France
Chromatographia (Impact Factor: 1.44). 01/2005; 61(1):55-60. DOI: 10.1365/s10337-004-0464-9

ABSTRACT Capillary electrochromatography (CEC) has been performed with a series of C 14, methlyacrylamide based monolithic columns. These monoliths with different porosities were prepared by in-situ copolymerization in fused-silica capillaries. The porous properties of monoliths were further observed using scanning electron micrographs (SEM) and measured using a mercury porosimeter. The effect of various alcohols as porogens on porous structural properties and chromatographic behaviors were also investigated. The effects of organic additive, pH value and ionic strength in mobile phase on electroosmosis flow (EOF) and separation were further discussed. Meanwhile, the baseline separation of 6 neutral compounds can be well obtained. In addition, the monolithic column demonstrates the high column efficiency and satisfactory reproducibility.