Preparation and Porous Property of C 14 Monolithic Column for Capillary Electrochromatography

State Key Laboratory of Elemento-Organic Chemistry, Nankai University, T’ien-ching-shih, Tianjin Shi, China
Chromatographia (Impact Factor: 1.41). 01/2005; 61(1):55-60. DOI: 10.1365/s10337-004-0464-9


Capillary electrochromatography (CEC) has been performed with a series of C 14, methlyacrylamide based monolithic columns. These monoliths with different porosities were prepared by in-situ copolymerization in fused-silica capillaries. The porous properties of monoliths were further observed using scanning electron micrographs (SEM) and measured using a mercury porosimeter. The effect of various alcohols as porogens on porous structural properties and chromatographic behaviors were also investigated. The effects of organic additive, pH value and ionic strength in mobile phase on electroosmosis flow (EOF) and separation were further discussed. Meanwhile, the baseline separation of 6 neutral compounds can be well obtained. In addition, the monolithic column demonstrates the high column efficiency and satisfactory reproducibility.

Download full-text


Available from: Chao Yan, Jul 09, 2015
  • [Show abstract] [Hide abstract]
    ABSTRACT: Recent advances in the CE and CEC separation, detection, and sample preparation methodologies applied to the determination of a variety of compounds having current or potential environmental relevance have been overviewed. The reviewed literature has illustrated the wide range of CE applications, indicating the continuing interest in CE and CEC in the environmental field.
    Electrophoresis 01/2006; 27(1):304-22. DOI:10.1002/elps.200500547 · 3.03 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: The column technologies play a crucial role in the development of new methods and technologies for the separation of biological samples containing hundreds to thousands compounds. This review focuses on the development of monolithic technology in micro-column formats for biological analysis, especially in capillary liquid chromatography, capillary electrochromatography and microfluidic devices in the past 5 years (2002-2007) since our last review in 2002 on monoliths for HPLC and CEC. The fabrication and functionalization of monoliths were summarized and discussed, with the aim of presenting how monolithic technology has been playing as an attractive tool for improving the power of existing chromatographic separation processes. This review consists of two parts: (i) the recent development in fabrication of monolithic stationary phases from direct synthesis to post-functionalization of the polymer- and silica-based monoliths tailoring the physical/chemical properties of porous monoliths; (ii) the application of monolithic stationary phases for one- and multi-dimensional capillary liquid chromatography, fast separation in capillary electro-driven chromatography, and microfluidic devices.
    Journal of Chromatography A 04/2008; 1184(1-2):369-92. DOI:10.1016/j.chroma.2007.09.022 · 4.17 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: The use of high internal phase emulsion polymers (polyHIPEs) for CEC applications has remained relatively unexplored. A few reports exist in the literature for the preparation of similar structures. In this study, polyHIPEs having high porosity, and interconnected open-cell structure, were introduced and evaluated as stationary phase for CEC. The polyHIPE monolithic columns were prepared by the in situ polymerization of isodecylacrylate (IDA) and divinylbenzene (DVB) in the continuous phase of a high internal phase emulsion (HIPE). Due to its well-defined polyHIPE structure with interconnected micron size spherical voids, the columns synthesized with different initiator concentrations were successfully used for the separation of alkylbenzenes. Furthermore, the columns indicated a strong electroosmotic flow (EOF) without any additional EOF generating monomer probably due to the presence of ionizable sulfate groups coming from the water-soluble initiator used in the preparation of polyHIPE matrix. The best chromatographic performance in the separation of alkylbenzenes was achieved by using 70% ACN in the mobile phase with high column efficiency (up to 200,000 plates/m).
    Journal of Chromatography A 03/2010; 1217(10):1654-9. DOI:10.1016/j.chroma.2010.01.020 · 4.17 Impact Factor
Show more