Article

PCR primers for trinucleotide and tetranucleotide microsatellites in greater amberjack, Seriola dumerili: PRIMER NOTE

Molecular Ecology Notes (Impact Factor: 2.38). 12/2006; 6(4):1162-1164. DOI: 10.1111/j.1471-8286.2006.01474.x
Source: OAI

ABSTRACT Eighteen nuclear-encoded microsatellites from a genomic DNA library of greater amberjack, Seriola dumerili, were isolated and characterized. The microsatellites include 13 perfect (five tetranucleotide and eight trinucleotide) and five imperfect (three tetranucleotide, one trinucleotide and one combination dinucleotide/trinucleotide) repeat motifs. The number of alleles at the 18 microsatellites among a sample of 29 fish ranged from two to 20; gene diversity (expected heterozygosity) ranged from 0.068 to 0.950, whereas observed heterozygosity ranged from 0.069 to 0.966. Following Bonferroni correction, genotypes at all 18 microsatellites fit expectations of Hardy-Weinberg equilibrium, and all pairwise comparisons of microsatellites did not deviate significantly from genotypic equilibrium. Greater amberjack support commercial and recreational fisheries along both the Atlantic and the Gulf coasts of the USA and represent a species with potential for worldwide aquaculture. The microsatellites developed will be useful for population genetic studies of 'wild' populations and breeding studies of domesticated populations.

0 Bookmarks
 · 
38 Views
  • [Show abstract] [Hide abstract]
    ABSTRACT: Expansion of finfish aquaculture will see increased incentive for translocation of stocks across geopolitical boundaries. The potential for genetic contamination of stocks arising from translocation and subsequent release or escape of translocated and/or genetically mixed stocks may be a significant risk to wild populations. Assessment of risk requires knowledge of the local population structure for the species across the range that translocation might occur and it is in this context we undertook a population genetic survey of stocks of Yellowtail Kingfish across temperate Australia and New Zealand. Seven polymorphic microsatellite loci were used to analyse a total of 272 individuals sampled from New Zealand, New South Wales, Victoria, South Australia and Western Australia. A subset of individuals from each sample locality was also assessed for variation at the mitochondrial gene ND4. Pairwise analysis of sample location and Bayesian analysis showed that Western Australia S. lalandi were genetically distinct from S. lalandi sampled from the other localities. No difference was found between New Zealand S. lalandi and eastern (New South Wales) or central (South Australia and Victoria) Australian fish. The mitochondrial analysis supported the microsatellite data with western samples possessing unique haplotypes compared with all other sites which shared haplotypes. With the expansion of the Yellowtail Kingfish aquaculture industry and likely translocation of stocks, there is a need for a review of translocation policies that consider genetic diversity as a factor in the development of Yellowtail Kingfish aquaculture in the region.
    Aquaculture 10/2011; 319(3):328-336. · 1.83 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Polymerase chain reaction primer pairs for a total of 25 nuclear-encoded microsatellites (loci) were developed from genomic DNA libraries of lane snapper ( Lutjanus synagris ), mutton snapper ( Lutjanus analis ), and yellowtail snapper ( Ocyurus chrysurus ). The micro- satellites include 24 perfect (21 dinucleotide and three trinucleotide) and one imperfect (combination tetranucleotide/tetranucleotide) repeat motifs. A total of 32 individuals of each species were assayed for allelic variation at all 25 microsatellites; reliable amplifica- tion products were generated for lane snapper (25 loci), mutton snapper (21 loci), and yel- lowtail snapper (24 loci). Significant deviations from Hardy-Weinberg expectations, following Bonferroni corrections, were found for one microsatellite in lane and yellowtail snappers, and for three microsatellites in mutton snapper. All pairwise comparisons of mic- rosatellites (all three species) did not deviate significantly from genotypic equilibrium.
    Molecular Ecology Notes 11/2007; 7(6):1084-1087. · 2.38 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Thirty-one microsatellite markers that were previously isolated from and characterized in greater amberjacks Seriola dumerili were assayed for cross-species amplification in four other members of the carangid genus Seriola: the lesser amberjack S. fasciata, yellowtail jack S. lalandi, almaco jack S. rivoliana, and banded rudderfish S. zonata. The number of markers that consistently amplified and were polymorphic ranged from 16 in yellowtail jacks to 25 in lesser amberjacks. The microsatellites characterized in this study will be useful for a variety of applications, including stock structure assessments of wild fish and parentage assignments of farmed fish.Received February 7, 2012; accepted April 16, 2012
    North American Journal of Aquaculture 10/2012; 74(4). · 0.71 Impact Factor

Full-text (3 Sources)

Download
33 Downloads
Available from
May 17, 2014