PCR primers for trinucleotide and tetranucleotide microsatellites in greater amberjack, Seriola dumerili: PRIMER NOTE
ABSTRACT Eighteen nuclear-encoded microsatellites from a genomic DNA library of greater amberjack, Seriola dumerili, were isolated and characterized. The microsatellites include 13 perfect (five tetranucleotide and eight trinucleotide) and five imperfect (three tetranucleotide, one trinucleotide and one combination dinucleotide/trinucleotide) repeat motifs. The number of alleles at the 18 microsatellites among a sample of 29 fish ranged from two to 20; gene diversity (expected heterozygosity) ranged from 0.068 to 0.950, whereas observed heterozygosity ranged from 0.069 to 0.966. Following Bonferroni correction, genotypes at all 18 microsatellites fit expectations of Hardy-Weinberg equilibrium, and all pairwise comparisons of microsatellites did not deviate significantly from genotypic equilibrium. Greater amberjack support commercial and recreational fisheries along both the Atlantic and the Gulf coasts of the USA and represent a species with potential for worldwide aquaculture. The microsatellites developed will be useful for population genetic studies of 'wild' populations and breeding studies of domesticated populations.
Full-textDOI: · Available from: John C Patton, Sep 28, 2015
- SourceAvailable from: Larry G. Allen
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- "Fifteen microsatellite loci developed for Seriola were used in this study: Sdu gA3D, Sdu CA4j, Sdu gA2D (Porta et al. 2009); Sdu 46, Sdu 32, Sdu 31, Sdu 10, Sdu 4 (Renshaw et al. 2006, 2007); Sequ 320, Sequ 230, Sequ 77, Sequ 58, Sequ 56, Sequ 38 (Ohara et al. 2003, 2005); and Sdn 03 (Nugroho and Taniguchi 1999). Microsatellites were combined into multiplex PCR sets based on compatible annealing temperatures and fragment sizes (Table S1). "
ABSTRACT: Recent study suggest the globally distributed yellowtail amberjack, Seriola lalandi sensu lato, is a complex of three closely related species. Together, these and three other species of Seriola comprise an important component of global aquaculture production with an esti- mated annual value of $1.3 billion. As yellowtail aqua- culture grows, the impact of unintentional releases on wild populations has become an increasingly important issue, particularly in light of international trade of hatchery seed. To create a genetic baseline, we examined spatial genetic structure in 260 specimens collected from seven locations over a wide geographical range using 15 nuclear microsatellites and mitochondrial control region sequences. Overall genetic differentiation among locations, as revealed by microsatellite data, was highly significant (FST = 0.085, DEST = 0.382, P \ 0.001), and pairwise estimates of divergence derived from mitochondrial and microsatellite data support the presence of four significantly differentiated populations corresponding to the N.E. Pacific, N.W. Pacific, S. Pacific, and South Atlantic. Based on the genetic differentiation detected in this study, and recently published sequence data, these populations more accurately reflect the presence of at least three cryptic species of Seriola. Especially strong genetic differentiation between hemispheres indicates that the equatorial region is a significant dispersal barrier for yellowtail. This study represents the broadest geographic investigation of genetic population structure conducted, to date, for specimens of the S. lalandi complex.Conservation Genetics 07/2015; DOI:10.1007/s10592-015-0755-8 · 2.19 Impact Factor
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- "The fluorescently labeled primer is in bold text, with the appropriate label signified by the number of plus signs (+ = 6-FAM; ++ = HEX; +++ = NED). Asterisks indicate information that was taken from earlier descriptions of these microsatellites (Renshaw et al. 2006 for Sdu1–Sdu27; Renshaw et al. 2007 for Sdu29–Sdu46); all information included for greater amberjacks was published previously (Renshaw et al. 2006, 2007). " N/A " indicates that the marker failed to amplify consistently. "
ABSTRACT: Thirty-one microsatellite markers that were previously isolated from and characterized in greater amberjacks Seriola dumerili were assayed for cross-species amplification in four other members of the carangid genus Seriola: the lesser amberjack S. fasciata, yellowtail jack S. lalandi, almaco jack S. rivoliana, and banded rudderfish S. zonata. The number of markers that consistently amplified and were polymorphic ranged from 16 in yellowtail jacks to 25 in lesser amberjacks. The microsatellites characterized in this study will be useful for a variety of applications, including stock structure assessments of wild fish and parentage assignments of farmed fish.Received February 7, 2012; accepted April 16, 2012North American Journal of Aquaculture 10/2012; 74(4). DOI:10.1080/15222055.2012.686959 · 0.68 Impact Factor
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ABSTRACT: Polymerase chain reaction primer pairs for a total of 25 nuclear-encoded microsatellites (loci) were developed from genomic DNA libraries of lane snapper ( Lutjanus synagris ), mutton snapper ( Lutjanus analis ), and yellowtail snapper ( Ocyurus chrysurus ). The micro- satellites include 24 perfect (21 dinucleotide and three trinucleotide) and one imperfect (combination tetranucleotide/tetranucleotide) repeat motifs. A total of 32 individuals of each species were assayed for allelic variation at all 25 microsatellites; reliable amplifica- tion products were generated for lane snapper (25 loci), mutton snapper (21 loci), and yel- lowtail snapper (24 loci). Significant deviations from Hardy-Weinberg expectations, following Bonferroni corrections, were found for one microsatellite in lane and yellowtail snappers, and for three microsatellites in mutton snapper. All pairwise comparisons of mic- rosatellites (all three species) did not deviate significantly from genotypic equilibrium.Molecular Ecology Notes 11/2007; 7(6):1084-1087. DOI:10.1111/j.1471-8286.2007.01785.x · 2.38 Impact Factor