Chromosome-Specific Single-Locus FISH Probes Allow Anchorage of an 1800-Marker Integrated Radiation-Hybrid/Linkage Map of the Domestic Dog Genome to All Chromosomes

Genetics Section, Animal Health Trust, Lanwades Park, Kentford, Newmarket, Suffolk CB8 7UU, UK.
Genome Research (Impact Factor: 14.63). 10/2001; 11(10). DOI: 10.1101/gr.189401
Source: PubMed Central


We present here the first fully integrated, comprehensive map of the canine genome, incorporating detailed cytogenetic, radiation hybrid (RH), and meiotic information. We have mapped a collection of 266 chromosome-specific cosmid clones, each containing a microsatellite marker, to all 38 canine autosomes by fluorescence in situ hybridization (FISH). A 1500-marker RH map, comprising 1078 microsatellites, 320 dog gene markers, and 102 chromosome-specific markers, has been constructed using the RHDF5000-2 whole-genome radiation hybrid panel. Meiotic linkage analysis was performed, with at least one microsatellite marker from each dog autosome on a panel of reference families, allowing one meiotic linkage group to be anchored to all 38 dog autosomes. We present a karyotype in which each chromosome is identified by one meiotic linkage group and one or more RH groups. This updated integrated map, containing a total of 1800 markers, covers >90% of the dog genome. Positional selection of anchor clones enabled us, for the first time, to orientate nearly all of the integrated groups on each chromosome and to evaluate the extent of individual chromosome coverage in the integrated genome map. Finally, the inclusion of 320 dog genes into this integrated map enhances existing comparative mapping data between human and dog, and the 1000 mapped microsatellite markers constitute an invaluable tool with which to perform genome scanning studies on pedigrees of interest.

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    • "We amplified a panel of 14 microsatellite markers (13 autosomal and one Y chromosome marker). These were FH2001, FH2010, FH2017, FH2054, FH2088, FH2096, FH2422 (Breen et al. [26]), FH3313, FH3725 (Guyon et al. [27]), PEZ06, PEZ08, PEZ15, PEZ19 (Halverson J. in Neff et al. [28]), and the Y-chromosome marker MS41B (Sundquist et al. [29]). We genotyped n = 477 faecal samples. "
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    BMC Ecology 06/2014; 14(11). DOI:10.1186/1472-6785-14-11 · 2.36 Impact Factor
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    Journal of applied genetics 05/2014; 55(4). DOI:10.1007/s13353-014-0217-x · 1.48 Impact Factor
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    • "The PCR conditions are provided in Supplementary Information. For coyotes, nine microsatellite loci (FH2001, FH2054, FH2088, FH2137, FH2611, FH2670, FH3725, C09.173 and Cxx.119 – Breen et al. 2001; Guyon et al. 2003; Holmes et al. 1994) based on the methods of Stenglein et al. (2010) and two sex-determining loci (DBX6 and DBY7 – Seddon 2005) were combined in one canid PCR multiplex. For PCR conditions, see Supplemental Information. "
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