Synthetic dye decolourization, textile dye and paper industrial effluent treatment using white rot fungi Lentines edodes
Desalination and water treatment (Impact Factor: 1.17). 04/2009; 4(1-3):143-147. DOI: 10.5004/dwt.2009.369
The laccase producing fungi Lentines edodes was screened for laccase production using various indicator compounds like guaiacol, tannic acid and the polymeric dyes such as Remazol brilliant blue R and Poly R-478. The organism Lentines edodes that produce laccase was cultivated on basal medium. Potato dextrose agar medium (PDA) and malt extract agar medium (MEA) were used for the first subculture of chosen isolate and the plates were examined by light microscopy to check the absence of bacteria and the unique fungi isolation. For testing the crude enzyme laccase activity an agar plug (1 cm2) from a 7-day old Lentines edodes agar plate was transferred to 50 ml of potato dextrose broth in Erlenmeyer flasks. The cultures were maintained at 25°C for 7 days. Screening was performed in Petri dishes (60 mm diameter) with 15 ml of malt extract agar (MEA) medium and potato dextrose agar (PDA) medium from Hi media, Mumbai, India, containing indicator compounds such as 0.04% (w/v) of Remazol Brilliant Blue-R (RBBR) and Poly R-478, 0.01% (v/v) of guaiacol and tannic acid 0.05% (w/v). Guaiacol (Sigma) RBBR (Sigma) and Poly R- 478 (Sigma) were added to the media after autoclaving as sterile-filtered solutions. Tannic acid (Merck Chemicals Ltd., UK) was autoclaved separately before addition to the media. Guaiacol is a sensitive substrate that allows a rapid screening of fungal strains producing extracellular guaiacol oxidizing enzymes by means of a colour reaction. The white-rot fungus Trametes hirsuta that produces laccase, manganese peroxidase and lignin peroxidase was used as a positive control. The identity of laccase producing fungal species was confirmed by brown color development surrounding the fungal growth. Any colony produce yellow or which causes decolourization of Poly R-478 was considered as ligninolytic positive and isolated. Decolourization of Poly R 478, RBBR, guaiacol and tannic acid oxidizing strains were also studied on liquid cultures for lignin peroxidase, manganese peroxidase and laccase activities. For laccase activity, the isolated fungi was grown at 25°C for 12 days with rotary shaking (150 rpm) in 500-ml baffled Erlenmeyer flasks containing 50 ml of potato dextrose broth. The crude extract of Lentines edodes revealed promising results on decolourization of various dye stuffs, paper and textile effluents. About 74.1% of reactive yellow, 77.5% reactive blue and 75% RBBR dye stuffs were effectively decolorized on the third day. Similarly, more than 90% of textile and paper effluents were decolorized on first day itself.
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ABSTRACT: An isolated fungi Aspergillus niger was found to be an effective decolourizing agent for wastewaters containing ink of sepia under aerobic conditions. It was found that decolourization of sepia ink by A. niger biomass includes two important processes: biosorption and biodegradation. Results showed that the entire black colour was found to be strongly bioadsorbed to the settling spherical fungal biomass pellets of A. niger. An optimisation of decolourization conditions using A. niger was quite beneficial for colour removal. The study revealed that maximum biosorption using A. niger biomass was obtained after 24 h of culture in liquid synthetic media (LSM) containing glucose as carbon source (1 g/L), mineral elements, sepia ink (0.5 g/L) and pH between 4.0 and 5.0. The process of decolourization is concomitant with the growth phase of the fungus and has a necessary requirement for a biodegradable substrate such as glucose. The results showed the capacities of A. niger biomass to degrade 3 g/L sepia ink containing in LSM in 96 h in optimal conditions and colour removal reached 96%.Desalination and water treatment 08/2010; 20(1-3):144-153. DOI:10.5004/dwt.2010.1168 · 1.17 Impact Factor
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ABSTRACT: In this study, removal of two cationic dyes, basic blue 9 (BB9) and basic violet 3 (BV3) with Rosa canina galls (RCG), was investigated. The parameters that affect the biosorption process such as pH of the solution, amount of biosorbent and initial dye concentration were studied. The kinetic and thermodynamic parameters of biosorption were calculated with batch systems. The optimum pH for the adsorption system was 5.0 and 7.0 for BB9 and BV3, respectively. The adsorption process followed the Freundlich model and pseudo-second-order kinetics. The maximum adsorption capacities were 107.53 mg g and 312.50 mg g for BB9 and BV3, respectively. The thermodynamic study indicated that the adsorptions of these cationic dyes were spontaneous and endothermic. The results show that RCG has a potential as an effective low-cost biosorbent for removal of cationic dyes.Desalination and water treatment 04/2012; 43(1-3):63-75. DOI:10.1080/19443994.2012.672203 · 1.17 Impact Factor
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ABSTRACT: Laccases are versatile enzymes belonging to the group of oxidases. Laccases catalyzes variety of phenolic compounds, as well as, diamines and aromatic amines with concomitant reduction of molecular oxygen to water. Laccases have mostly been isolated and characterized from plants and fungi in contrast, little is known about bacterial laccases. Thermostability of this particular enzyme makes an attractive feature for their biotechnological application, especially due to its extensive and advanced applications. The applications includes effluents detoxification from the paper and pulp industries, textile industries, petrochemical industries, food, cosmetics, soil bioremediation and biodegradation of environmental phenolic pollutants. The removal of xenobiotic substances and production of polymeric products makes them a useful candidate for bioremediation purposes. In the last few decades, laccase received much attention due to its ability to oxidize both phenolic and non-phenolic compounds. The present review summarizes the distribution of bacterial laccases and their overview in industrial applications in different sectors. © 2014 Asian Network for Scientific Information.Biotechnology(Faisalabad) 05/2014; 13(5):196-205. DOI:10.3923/biotech.2014.196.205