Precolumn derivatization of reducing carbohydrates with 4-(3Methyl5-oxo-2-pyrazolin-1-yl) benzoic acid. Study of reaction, high-performance liquid chromatographic separation and quantitative performance of method

Chromatographia (Impact Factor: 1.41). 08/2002; 56(3):153-160. DOI: 10.1007/BF02493204


Summary A simple method for quantitative determination of carbohydrates by reversed-phase, high-performance liquid chromatography
after pre-column derivatization and UV detection has been developed. Reducing sugars are condensed with 4-(3-methyl-5-oxo-2-pyrazolin-l-yl)
benzoic acid (PMPA) to yield UV adducts absorbing at 271 nm, which are resolved under typical reversed-phase conditions. After
derivatization, excess PMPA is easily removed from the reaction mixture by precipitation with mineral acids at pH

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