Article

[Molecular cloning and characterization of Ras-homologues from different developing stages of Echinococcus granulosus].

Key Laboratory of Xinjiang Hydatid Institute, The First Teaching Hospital of Xinjiang Medical University, Urumqi 830054, China.
Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases 03/2009; 27(1):91-3. pp.91-3
Source: PubMed

ABSTRACT The Ras GTPase gene from protoscolex and adult worm of Echinococcus granulosus in Xinjiang were cloned by RT-PCR and named as Eg Ras-pro (GenBank No. EU560397) and Eg Ras-adult (GenBank No. EU560398). Sequence analysis showed that each gene had 552 bp, coding 184 aa with an pI of 6.54. Bioinformatics analysis revealed that Eg Ras-pro and Eg Ras-adult had 98.4% and 98.9% homology to Echinococcus multilocularis Ras and 53.9%-78.8% homology to other species. Phylogenetic analysis showed that Eg Ras-pro and Eg Ras-adult clustered with EmRas and SmRas. The data indicated that EgRas GTPase has been expressed from protoscolex and adult worm of Echinococcus granulosus, and both are highly conservative.

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Keywords

6.54. Bioinformatics analysis
 
adult worm
 
Echinococcus granulosus
 
Echinococcus multilocularis Ras
 
EgRas GTPase
 
EmRas
 
GenBank
 
Phylogenetic analysis
 
pI
 
Ras GTPase gene
 
Ras-adult
 
Ras-pro
 
Sequence analysis
 
SmRas